Down-regulation of lncRNA SNHG5 relieves sepsis-induced acute kidney injury by regulating the miR-374a-3p/TLR4/NF-κB pathway

Wei, Min; Wei, Jing; Shang, Futai; Zang, Kui; Zhang, Peng

doi:10.1093/jb/mvab008

Cited by 24 publications

(20 citation statements)

References 32 publications

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“…Beside miR-320, there are many other miRNAs that were strongly deregulated in the patient populations. Of the most upregulated miRNAs, miR-374a-3p and miR-15a-3p are involved in acute kidney and neuroinflammation, respectively, whereas mir-4721 was proposed as one of the miRNA molecular diagnostic markers for liver fibrosis in patients with chronic hepatitis B [ 36 , 37 , 38 ]. Of the most downregulated miRNAs, miR-4747-3p was found upregulated in Alzheimer’s Disease and hsa-miR-4429 was suggested as a potential diagnostic biomarker for biliary atresia [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

The MiR-320 Family Is Strongly Downregulated in Patients with COVID-19 Induced Severe Respiratory Failure

Duecker

Adam

Wirtz

et al. 2021

IJMS

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A high incidence of thromboembolic events associated with high mortality has been reported in severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infections with respiratory failure. The present study characterized post-transcriptional gene regulation by global microRNA (miRNA) expression in relation to activated coagulation and inflammation in 21 critically ill SARS-CoV-2 patients. The cohort consisted of patients with moderate respiratory failure (n = 11) and severe respiratory failure (n = 10) at an acute stage (day 0–3) and in the later course of the disease (>7 days). All patients needed supplemental oxygen and severe patients were defined by the requirement of positive pressure ventilation (intubation). Levels of D-dimers, activated partial thromboplastin time (aPTT), C-reactive protein (CRP), and interleukin (IL)-6 were significantly higher in patients with severe compared with moderate respiratory failure. Concurrently, next generation sequencing (NGS) analysis demonstrated increased dysregulation of miRNA expression with progression of disease severity connected to extreme downregulation of miR-320a, miR-320b and miR-320c. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis revealed involvement in the Hippo signaling pathway, the transforming growth factor (TGF)-β signaling pathway and in the regulation of adherens junctions. The expression of all miR-320 family members was significantly correlated with CRP, IL-6, and D-dimer levels. In conclusion, our analysis underlines the importance of thromboembolic processes in patients with respiratory failure and emphasizes miRNA-320s as potential biomarkers for severe progressive SARS-CoV-2 infection.

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Section: Discussionmentioning

confidence: 99%

The MiR-320 Family Is Strongly Downregulated in Patients with COVID-19 Induced Severe Respiratory Failure

Duecker

Adam

Wirtz

et al. 2021

IJMS

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“…However, with the silencing of SNHG5, all these adverse effects caused by high glucose can be improved accordingly. Indeed, downregulation of SNHG5 can inhibit the levels of various inflammatory factors in HK-2 cells, which has been proved by Wang et al [14]. Taken together, downregulation of SNHG5 can alleviate the damage caused by hyperglycemia to HK2 cells to some extent.…”

Section: Discussionmentioning

confidence: 67%

“…Similarly, the SNHG5 level of NCM460 cells cultured with high glucose and high lipid in vitro was significantly increased, and the upregulated SNHG5 inhibited cell proliferation and promoted cell apoptosis markedly [13]. Another study revealed that SNHG5 may play a regulatory role in sepsis induced acute kidney injury by regulating the miR-374a-3p/TLR4/NF-κB pathway [14]. To some extent, these studies have shown evidence of SNHG5 and diabetes and kidney damage, but up to now, there are still relatively few studies on the relationship between SNHG5 and DN.…”

Section: Introductionmentioning

confidence: 95%

Long Non-Coding RNA Small Nucleolar RNA Host Gene 5 (SNHG5) Regulates Renal Tubular Damage in Diabetic Nephropathy via Targeting MiR-26a-5p

et al. 2021

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The study explored the diagnostic value of SNHG5 in diabetic nephropathy (DN) and investigated the role and mechanism on DN via establishing the in vitro HK2 cell model. This study recruited 62 types 2 diabetes mellitus (T2DM) patients, 58 DN patients and 60 healthy controls (HC). The expressions of serum SNHG5 and miR-26a-5p were measured by RT-qPCR analysis. The diagnostic value of SNHG5 in DN was assessed by ROC curve. The in vitro cell model was built to estimate the effects of SNHG5 on cell viability, cell apoptosis, inflammation response and oxidative stress. Serum SNHG5 was increased in DN patients (relative expression: 2.04±0.34) and had the diagnostic value in DN. After HK2 cells were treated with high glucose, the cell viability decreased and apoptosis increased, and the production of inflammatory cytokines and ROS enhanced significantly. It was noticed that inhibition of SNHG5 could reverse the above phenomenon caused by high glucose. Besides, serum miR-26a-5p was diminished in DN patients, and luciferase reporter gene revealed that miR-26a-5p is direct target of SNHG5. These results indicated that inhibition of SNHG5 may mitigate HG-induced renal tubular damage via targeting miR-26a-5p, which providing a new insight into the mechanism of renal tubule damage in DN patients.

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“…Earlier studies showed that IGFALS might be a useful diagnostic and therapeutic target for HCC [37] and that SORBS2 can accurately predict prognosis of HCC patients [38]. IGFALS has also been identified as a tumor suppressor gene, which is silenced by methylation in HCC [39,40].…”

Section: Discussionmentioning

confidence: 99%

Prognosis-related molecular subtypes and immune features associated with hepatocellular carcinoma

Lin

Gao

et al. 2022

Preprint

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Bioinformatics tools were used to identify prognosis-related molecular subtypes and biomarkers of hepatocellular carcinoma (HCC). Differential expression analysis of four datasets (TCGA, GSE76427, GSE25097, and GSE14520) identified 3,330 genes differentially expressed in the same direction in all four datasets; those genes were involved in the cell cycle, FOXO signaling pathway, as well as complement and coagulation cascades. Based on non-negative matrix decomposition, two molecular subtypes of HCC with different prognosis were identified, with subtype C2 showing better overall survival than subtype C1. Cox regression and Kaplan-Meier analysis showed that 217 of the overlapping DEGs were closely associated with HCC prognosis. The subset of those genes showing an area under the curve > 0.80 was used to construct random survival forest and least absolute shrinkage and selection operator models, which identified seven feature genes (SORBS2, DHRS1, SLC16A2, RCL1, IGFALS, GNA14, and FANCI) that may be involved in HCC occurrence and prognosis. Based on the feature genes, risk score and recurrence models were constructed, while a univariate Cox model identified FANCI as a key gene involved mainly in the cell cycle, DNA replication, and mismatch repair. Further analysis showed that FANCI had two mutation sites and that its gene may undergo methylation. Single-sample gene set enrichment analysis showed that Th2 and T helper cells are significantly upregulated in HCC patients compared to controls. Our results identify FANCI as a potential prognostic biomarker for HCC.

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Down-regulation of lncRNA SNHG5 relieves sepsis-induced acute kidney injury by regulating the miR-374a-3p/TLR4/NF-κB pathway

Cited by 24 publications

References 32 publications

The MiR-320 Family Is Strongly Downregulated in Patients with COVID-19 Induced Severe Respiratory Failure

The MiR-320 Family Is Strongly Downregulated in Patients with COVID-19 Induced Severe Respiratory Failure

Long Non-Coding RNA Small Nucleolar RNA Host Gene 5 (SNHG5) Regulates Renal Tubular Damage in Diabetic Nephropathy via Targeting MiR-26a-5p

Prognosis-related molecular subtypes and immune features associated with hepatocellular carcinoma

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