Down-regulation of juvenile hormone esterase and arylphorin production inHeliothis virescens larvae parasitized byMicroplitis croceipes

Dong, Kun; Zhang, Deqing; Dahlman, D. L.

doi:10.1002/(sici)1520-6327(1996)32:2<237::aid-arch7>3.0.co;2-v

Cited by 39 publications

(16 citation statements)

References 34 publications

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“…Among the identified immune related DGEs, six unigenes encoding hexamerin (one up-regulate and five down-regulated) were found. Similarly, the transcriptional responses of hexamerins to parasitization have been documented in several host-parasitoid systems with different regulation strategies [76]–[79]. Although distinct regulatory mechanisms at transcriptional level may exist in different host–parasitoid systems, studies so far indicate that the titer of hexamerins in the hemolymph increase in the hosts following parasitism [80]–[83].…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Immune Response of Tenebrio molitor Pupae to Parasitization by Scleroderma guani

et al. 2013

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BackgroundHost and parasitoid interaction is one of the most fascinating relationships of insects, which is currently receiving an increasing interest. Understanding the mechanisms evolved by the parasitoids to evade or suppress the host immune system is important for dissecting this interaction, while it was still poorly known. In order to gain insight into the immune response of Tenebrio molitor to parasitization by Scleroderma guani, the transcriptome of T. molitor pupae was sequenced with focus on immune-related gene, and the non-parasitized and parasitized T. molitor pupae were analyzed by digital gene expression (DGE) analysis with special emphasis on parasitoid-induced immune-related genes using Illumina sequencing.Methodology/Principal FindingsIn a single run, 264,698 raw reads were obtained. De novo assembly generated 71,514 unigenes with mean length of 424 bp. Of those unigenes, 37,373 (52.26%) showed similarity to the known proteins in the NCBI nr database. Via analysis of the transcriptome data in depth, 430 unigenes related to immunity were identified. DGE analysis revealed that parasitization by S. guani had considerable impacts on the transcriptome profile of T. molitor pupae, as indicated by the significant up- or down-regulation of 3,431 parasitism-responsive transcripts. The expression of a total of 74 unigenes involved in immune response of T. molitor was significantly altered after parasitization.Conclusions/Significanceobtained T. molitor transcriptome, in addition to establishing a fundamental resource for further research on functional genomics, has allowed the discovery of a large group of immune genes that might provide a meaningful framework to better understand the immune response in this species and other beetles. The DGE profiling data provides comprehensive T. molitor immune gene expression information at the transcriptional level following parasitization, and sheds valuable light on the molecular understanding of the host-parasitoid interaction.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic Immune Response of Tenebrio molitor Pupae to Parasitization by Scleroderma guani

et al. 2013

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“…A sequence encoding a dynein light chain was 5 fold more abundant in the fat body of hosts infected by HdIV and MdBV, while a beta-tubulin was 2.5 fold less abundant. Alterations in host storage protein levels are also known to occur following parasitism by other PDV-carrying wasps (Beckage and Kanost,1993;Dong et al,1996;Shelby and Webb, 1994). The main targets of HdIV and MdBV were apolipophorin and arylphorin which decreased up to 3.6 times in the fat body.…”

Section: Gene Namementioning

confidence: 99%

Lepidopteran transcriptome analysis following infection by phylogenetically unrelated polydnaviruses highlights differential and common responses

Provost

Jouan

Hilliou

et al. 2011

Insect Biochemistry and Molecular Biology

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“…Briefly, 8 µL droplets of sample mixtures, consisting of either 3 µL of control (buffer used in re-suspending TSP14) or TSP14 (3.6 µg) and 5 µL of 10% sucrose (dissolved in Grace's medium), were placed randomly inside a plastic Midgut labelling assays were modified from Dong et al (1996). Individual midguts from fourth and fifth instar H.…”

Section: Insect Bioassaysmentioning

confidence: 99%

Antibiosis‐type insect resistance in transgenic plants expressing a teratocyte secretory protein (TSP14) gene from a hymenopteran endoparasite (Microplitis croceipes)

Maiti¹,

Dey

Pattanaik³

et al. 2003

Plant Biotechnology Journal

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SummaryWe have isolated a teratocyte secretory protein (TSP14) gene product from a hymenopteran endoparasite that disrupts the growth of lepidopteran insect larvae. To evaluate the insecticidal activity of TSP14 for the protection of crops from insect damage, chimeric gene constructs of TSP14 were expressed in transgenic plants. The coding sequence of the TSP14 gene, with and without its native signal peptide, was placed between the modified peanut chlorotic streak virus (PClSV) full-length transcript (FLt) promoter with duplicated enhancer domains and the terminator sequence from the rbcSE9 gene. These chimeric genes, expressed in transgenic tobacco (Nicotiana tabacum cv. Samsun NN) were stably inherited in successive plant generations (R 0 , R 1 and R 2 progeny) as shown by molecular analysis.A Western blot analysis of plant extracts showed the presence of a polypeptide of the expected size that cross-reacted with TSP14-specific antibodies. Larvae of the tobacco budworm (Heliothis virescens) and tobacco hornworm (Manduca sexta) which were fed with several independent homozygous transgenic plant lines (R 2 progeny) exhibited mortality and reduced growth rates compared to those fed with plants transformed by a vector control. Our results demonstrate the potential for introduction of the TSP14 gene into plants in order to achieve protection against lepidopteran pests.

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Down-regulation of juvenile hormone esterase and arylphorin production inHeliothis virescens larvae parasitized byMicroplitis croceipes

Cited by 39 publications

References 34 publications

Transcriptomic Immune Response of Tenebrio molitor Pupae to Parasitization by Scleroderma guani

Transcriptomic Immune Response of Tenebrio molitor Pupae to Parasitization by Scleroderma guani

Lepidopteran transcriptome analysis following infection by phylogenetically unrelated polydnaviruses highlights differential and common responses

Antibiosis‐type insect resistance in transgenic plants expressing a teratocyte secretory protein (TSP14) gene from a hymenopteran endoparasite (Microplitis croceipes)

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