Down-regulation of biofilm-associated genes in mecA-positive methicillin-resistant S. aureus treated with M. communis extract and its antibacterial activity

Khaleghi, Moj; Khorrami, Sadegh

doi:10.1186/s13568-021-01247-z

Cited by 13 publications

(6 citation statements)

References 55 publications

(53 reference statements)

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“…Moreover, the extracted RNA was loaded on an agarose gel electrophoresis (1.5%) to confirm its quality. Thereafter, cDNA was synthesized utilizing Oligo‐dT primer and M‐MuLV reverse transcriptase enzyme (Fermentas), based on the manufacturer's instructions (Khaleghi & Khorrami, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Methyl jasmonate ameliorates pain‐induced learning and memory impairments through regulating the expression of genes involved in neuroinflammation

Mohammadinia,

Esmaeili‐Mahani,

Abbasnejad

et al. 2024

Brain and Behavior

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ObjectiveOrofacial pain with high prevalence is one of the substantial human health issues. The importance of this matter became more apparent when it was revealed that orofacial pain, directly and indirectly, affects cognition performances. Currently, researchers have focused on investigating pharmaceutics to alleviate pain and ameliorate its subsequent cognitive impairments.DesignIn this study, the rats were first treated with the central administration of methyl jasmonate (MeJA), which is an antioxidant and anti‐inflammatory bio‐compound. After 20 min, orofacial pain was induced in the rats by the injection of capsaicin in their dental pulp. Subsequently, the animals’ pain behaviors were analyzed, and the effects of pain and MeJA treatments on rats learning and memory were evaluated/compared using the Morris water maze (MWM) test. In addition, the expression of tumor necrosis factor‐α (TNF‐α), IL‐1β, BDNF, and COX‐2 genes in the rats’ hippocampus was evaluated using real‐time polymerase chain reaction.ResultsExperiencing orofacial pain resulted in a significant decline in the rats learning and memory. However, the central administration of 20 μg/rat of MeJA effectively mitigated these impairments. In the MWM, the performance of the MeJA‐treated rats showed a two‐ to threefold improvement compared to the nontreated ones. Moreover, in the hippocampus of pain‐induced rats, the expression of pro‐inflammatory factors TNF‐α, IL‐1β, and COX‐2 significantly increased, whereas the BDNF expression decreased. In contrast, MeJA downregulated the pro‐inflammatory factors and upregulated the BDNF by more than 50%.ConclusionsThese findings highlight the notable antinociceptive potential of MeJA and its ability to inhibit pain‐induced learning and memory dysfunction through its anti‐inflammatory effect.

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Section: Methodsmentioning

confidence: 99%

Methyl jasmonate ameliorates pain‐induced learning and memory impairments through regulating the expression of genes involved in neuroinflammation

Mohammadinia,

Esmaeili‐Mahani,

Abbasnejad

et al. 2024

Brain and Behavior

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“…The extract destroyed the pre-formed biofilm at sub-MIC concentration and affected the bacterial cells within the biofilm. The MRSA genes icaA, icaD, sarA, and bap, which are involved in biofilm formation and development, were significantly expressed less, which inhibited biofilm development (54).…”

Section: Antibiofilm Activitymentioning

confidence: 99%

Myrtus communis Leaves: Source of Bioactives, Traditional Use and their Biological Properties

Mir¹

2023

Preprint

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Myrtus communis L., commonly known as true myrtle, is a medicinal plant native to the Mediterranean area. Since ancient times inhabitants of this area have been using it for its cultural and medicinal properties. Due to the high content of essential oil in its flowers, leaves and fruits, M. communis is an important medicinal and aromatic species from Myrtaceae family. Because of the presence of vast diversity of biomolecules in its aerial parts, it exhibits several biological properties of antioxidant, antimicrobial and anticancer. There has been increasing scientific interest in the field to understand the pleotropic effects of its extracts or essential oils on various ailments and diseases. This purpose of this review is to summarizes the chemical composition, traditional uses, and biological activities of M. communis L. leaves documented in numerous recent studies.

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“…The extract destroyed the pre-formed biofilm at sub-MIC concentration and affected the bacterial cells within the biofilm. The inhibition of biofilm development was due to significant decrease in the expression of MRSA genes icaA, icaD, sarA, and bap, which are involved in biofilm formation and development (54).…”

Section: Antibiofilm Activitymentioning

confidence: 99%

Myrtus Communis Leaves; Source of Bioactives, Traditional Use and Their Biological Properties

Mir¹

2023

Preprint

View full text Add to dashboard Cite

Myrtus communis L., commonly known as true myrtle, is a medicinal plant native to the Mediterranean area. Since ancient times inhabitants of this area have been using it for its cultural and medicinal properties. Due to the high content of essential oil in its flowers, leaves and fruits, M. communis is an important medicinal and aromatic species from Myrtaceae family. Because of the presence of vast diversity of biomolecules in its aerial parts, it exhibits several biological properties of antioxidant, antimicrobial and anticancer. There has been increasing scientific interest in the field to understand the pleotropic effects of its extracts or essential oils on various ailments and diseases. This review summarizes the chemical composition, traditional uses, and biological activities of M. communis L. leaves documented in numerous recent studies.

show abstract

Down-regulation of biofilm-associated genes in mecA-positive methicillin-resistant S. aureus treated with M. communis extract and its antibacterial activity

Cited by 13 publications

References 55 publications

Methyl jasmonate ameliorates pain‐induced learning and memory impairments through regulating the expression of genes involved in neuroinflammation

Methyl jasmonate ameliorates pain‐induced learning and memory impairments through regulating the expression of genes involved in neuroinflammation

<em>Myrtus communis </em>Leaves: Source of Bioactives, Traditional Use and their Biological Properties

<strong><em></em></strong><em>Myrtus Communis </em>Leaves; Source of Bioactives, Traditional Use and Their Biological Properties

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