Double‐stranded RNA targeting calmodulin reveals a potential target for pest management of <scp><i>Nilaparvata lugens</i></scp>

Wang, Weixia; Wan, Pin-Jun; Li, Fengxiang; Zhu, Tongyu; Fu, Qiang

doi:10.1002/ps.4865

Cited by 30 publications

(32 citation statements)

References 48 publications

(52 reference statements)

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“…RNAi bioassay was performed by injection as previously reported ( Wang et al, 2018 ). Briefly, 200 ng (0.05 μL) and 400 ng (0.1 μL) dsRNA (concentration estimated 4.000 mg/mL) and the same amount of ds GFP (negative control) were injected into the newly emerged fourth- and fifth-instar nymphs (N4D1 and N5D1; Xu et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

The Roles of E93 and Kr-h1 in Metamorphosis of Nilaparvata lugens

Yuan

Nanda

et al. 2018

Front. Physiol.

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Metamorphosis is a crucial process in insect development. Ecdysone-induced protein 93 (E93) is a determinant that promotes adult metamorphosis in both hemimetabolous and holometabolous insects. Krüppel-homolog 1 (Kr-h1), an early juvenile hormone (JH)-inducible gene, participates in JH signaling pathway controlling insect metamorphosis. In the current study, an E93 cDNA (NlE93) and two Kr-h1 cDNA variants (NlKr-h1-a and NlKr-h1-b) were cloned from Nilaparvata lugens (Stål), one of the most destructive hemimetabolous insect pests on rice. Multiple sequence alignment showed that both NlE93 and NlKr-h1 share high identity with their orthologs from other insects. The expression patterns revealed that decreasing NlKr-h1 mRNA levels were correlated with increasing NlE93 mRNA levels and vice versa. Moreover, RNA interference (RNAi) assays showed that the knockdown of one of the two genes resulted in significantly upregulated expression of the other. Correspondingly, phenotypical observation of the RNAi insects revealed that depletion of NlE93 prevented nymph–adult transition (causing a supernumerary nymphal instar), while depletion of NlKr-h1 triggered precocious formation of incomplete adult features. The results suggest that Nlkr-h1 and NlE93 are mutual repressors, fitting into the MEKRE93 pathway. The balance between these two genes plays a critical role in the metamorphosis of N. lugens determining the proper timing for activating metamorphosis during the nymphal stage.

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Section: Methodsmentioning

confidence: 99%

The Roles of E93 and Kr-h1 in Metamorphosis of Nilaparvata lugens

Yuan

Nanda

et al. 2018

Front. Physiol.

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“…Therefore, the selection of the target to be silenced is a crucial element. Previously, silencing of genes involved in the metabolism of N. lugens , by injection or feeding of dsRNAs, has shown the potential of RNAi based pest control (Chen et al, ; Ge et al, ; Li et al, ; Wang, Wan, Lai, Zhu, & Fu, ).…”

Section: Introductionmentioning

confidence: 99%

The OST‐complex as target for RNAi‐based pest control in Nilaparvata lugens

Schutter

Chen

Shen

et al. 2019

Arch Insect Biochem Physiol

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RNAi-based pest control strategies are emerging as environment friendly and species-specific alternatives for the use of conventional pesticides. Because N-glycosylation is important for many biological processes, such as growth and development, the early steps of protein N-glycosylation are promising targets for an RNAi-based pest control strategy. Through injection of dsRNAs, the expression of the catalytic subunits of the oligosaccharyl transferase complex was efficiently silenced in nymphs of the notorious rice pest insect Nilaparvata lugens. Silencing of both STT3 isoforms resulted in a high mortality of the N. lugens nymphs.However, our data reveals the occurrence of a functional redundancy between the two isoforms when silencing only one of the isoforms. These observations confirm the potential to use the early genes in the N-glycosylation pathway as targets for an RNAi-based pest control strategy.In addition, the existence of a functional redundancy between the two STT3 isoforms presents a factor which one must take into account when designing RNAi-based approaches. K E Y W O R D S N-glycosylation, Nilaparvata lugens, oligosaccharyl transferase complex, pest control, RNAi Arch. Insect Biochem. Physiol. 2019;101:e21555.wileyonlinelibrary.com/journal/arch

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“…For example: In second-instar Euschistus heros , 27.4 ng dsRNA injection reduced the EhATPase A and Ehact-2 transcript levels by 90% and 59%, respectively, (Castellanos et al, 2018). In third-instar Nilaparvata lugens nymphs, the transcript levels of NlCaM and NlCaM1 at 4 days after injection of 50 ng dsRNA were reduced by 67.9% and 62.8%, respectively, (Wang et al, 2018). Finally, in Nephotettix cincticeps , the expression levels of NcSP75 had decreased to 0.2–0.5% of those of the control group at 2, 4, and 8 d after a 9 ng dsRNA-injection (Matsumoto and Hattori, 2018).…”

Section: Discussionmentioning

confidence: 99%

Induction of RNAi Core Machinery’s Gene Expression by Exogenous dsRNA and the Effects of Pre-exposure to dsRNA on the Gene Silencing Efficiency in the Pea Aphid (Acyrthosiphon pisum)

Jiang

et al. 2019

Front. Physiol.

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The pea aphid, Acyrthosiphon pisum, is an important agricultural pest and biological model organism, and RNA interference (RNAi) is an important tool for functional genomics and for insect pest management. However, the efficiency of RNAi in pea aphids is variable, limiting its application in aphids. In this study, we present optimized conditions for inducing and increasing the gene silencing efficiency of RNAi in pea aphids. The optimal gene silencing of the target Aphunchback gene was achieved by injecting 600 ng double-stranded (ds) RNA, and the highest mRNA depletion rate (74%) was detected at 36 h after injection. Moreover, the same gene silencing conditions were used to achieve transcript silencing for nine different genes in the pea aphid, although the silencing efficiencies for the different genes varied. Furthermore, the pre-exposure of aphids to dsRNA (600 ng dsGFP) led to significant hunchback silencing following a secondary exposure to 60 ng of dshunchback, a dose which did not lead to gene silencing when independently injected. The information presented here can be exploited to develop more efficient RNAi bioassays for pea aphids, both as gene functional study tools and an insect pest control strategy.

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Double‐stranded RNA targeting calmodulin reveals a potential target for pest management of Nilaparvata lugens

Abstract: NlCaM plays important roles mainly in nymph development and uptake of vitellogenin by ovaries in vitellogenesis in N. lugens. dsRNA derived from the less conserved 3'-UTR of NlCaM shows great potential for RNAi-based N. lugens management. © 2018 Society of Chemical Industry.

Cited by 30 publications

References 48 publications

The Roles of E93 and Kr-h1 in Metamorphosis of Nilaparvata lugens

The Roles of E93 and Kr-h1 in Metamorphosis of Nilaparvata lugens

The OST‐complex as target for RNAi‐based pest control in Nilaparvata lugens

Induction of RNAi Core Machinery’s Gene Expression by Exogenous dsRNA and the Effects of Pre-exposure to dsRNA on the Gene Silencing Efficiency in the Pea Aphid (Acyrthosiphon pisum)

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