ABSTRACTDouble-stranded RNA (dsRNA) recently has been shown to give rise to genetic interference in Caenorhabditis elegans and also is likely to be the basis for phenotypic cosuppression in plants in certain instances. While constructing a plasmid vector for transfection of trypanosome cells, we serendipitously discovered that in vivo expression of dsRNA of the ␣-tubulin mRNA 5 untranslated region (5 UTR) led to multinucleated cells with striking morphological alterations and a specific block of cytokinesis. …
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