Dose-response metabolomics and pathway sensitivity to map molecular cartography of bisphenol A exposure

Zhao, Haoduo; Liu, Min; Lv, Yunbo; Fang, Mingliang

doi:10.1016/j.envint.2021.106893

Cited by 22 publications

(7 citation statements)

References 55 publications

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“…This may suggest an enhanced lipid peroxidation as well as altered fatty acid β-oxidation impact on ATP anabolism and also lipid catabolism, which will be further discussed later. Collectively, LCMs induced global metabolome dysregulation in HK2 cells, which was featured in energy homeostasis, macromolecule metabolism dysregulation, oxidative stress response, and lipid peroxidation …”

Section: Resultsmentioning

confidence: 99%

“…metabolism dysregulation, oxidative stress response, and lipid peroxidation. 30 Transcriptomic Profiling. HiSeq RNA sequencing was adopted to screen the DEGs of HK2 cells induced by LCM 41 exposure.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…After rinsing with PBS twice rapidly, cells were collected with 1.6 mL of ice-cold methanol/acetonitrile/water (2:2:1, v/v/v) and harvested by a cell scraper. The metabolite extraction procedure was modified from our previous study. − A freeze–thaw step was conducted thrice by placing in liquid nitrogen for 2 min followed by sonication in an ice bath for 10 min in each cycle. After being placed at −40 °C for 1 h, the samples were vigorously vortexed and then centrifuged for 15 min at 13,000 rpm in 4 °C ambient temperature to precipitate proteins.…”

Section: Methodsmentioning

confidence: 99%

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Liquid Crystal Monomer: A Potential PPARγ Antagonist

Zhao

Naik

et al. 2023

Environ. Sci. Technol.

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Liquid crystal monomers (LCMs) are a large family of artificial ingredients that have been widely used in global liquid crystal display (LCD) industries. As a major constituent in LCDs as well as the end products of e-waste dismantling, LCMs are of growing research interest with regard to their environmental occurrences and biochemical consequences. Many studies have analyzed LCMs in multiple environmental matrices, yet limited research has investigated the toxic effects upon exposure to them. In this study, we combined in silico simulation and in vitro assay validation along with omics integration analysis to achieve a comprehensive toxicity elucidation as well as a systematic mechanism interpretation of LCMs for the first time. Briefly, the high-throughput virtual screen and reporter gene assay revealed that peroxisome proliferatoractivated receptor gamma (PPARγ) was significantly antagonized by certain LCMs. Besides, LCMs induced global metabolome and transcriptome dysregulation in HK2 cells. Notably, fatty acid β-oxidation was conspicuously dysregulated, which might be mediated through multiple pathways (IL-17, TNF, and NF-kB), whereas the activation of AMPK and ligand-dependent PPARγ antagonism may play particularly important parts. This study illustrated LCMs as a potential PPARγ antagonist and explored their toxicological mode of action on the trans-omics level, which provided an insightful overview in future chemical risk assessment.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Liquid Crystal Monomer: A Potential PPARγ Antagonist

Zhao

Naik

et al. 2023

Environ. Sci. Technol.

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“…BPA exposure altered nucleotide metabolism in the ERpositive cell line, MCF-7, evidenced by increases in the nucleotide precursors cytidine triphosphate (CTP) and uridine diphosphate (UDP) glucuronic acid. [74] Further supporting this result, a study that tested cocktails of exposures with a "leave one out" approach found that BPA was crucial for altering purine and pyrimidine metabolism in MCF-7 cells, evidenced by increases in UDP, uridine triphosphate, cytidine diphosphate, and CTP. [75] Nucleotides are essential for cellular viability and proliferation.…”

Section: Bisphenolsmentioning

confidence: 85%

“…In vitro BPA impacted nucleotide synthesis in MCF-7 cells [74] In vitro In a "leave one out" approach, BPA was shown to be necessary for inducing changes in purine and pyrimidine metabolism in MCF-7 [75] In vitro BPS dysregulated the citric acid cycle, purine metabolism, and lipid metabolism in MCF-10A cells. [76] In vitro TB-BPA and TC-BPA induced glycolytic intermediates and altered glutathione metabolism in MCF10A cells.…”

Section: Bisphenolsmentioning

confidence: 99%

Endocrine‐disrupting compounds and metabolomic reprogramming in breast cancer

Winz,

Zong,

Suh

2023

J Biochem & Molecular Tox

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Endocrine‐disrupting chemicals pose a growing threat to human health through their increasing presence in the environment and their potential interactions with the mammalian endocrine systems. Due to their structural similarity to hormones like estrogen, these chemicals can interfere with endocrine signaling, leading to many deleterious effects. Exposure to estrogenic endocrine‐disrupting compounds (EDC) is a suggested risk factor for the development of breast cancer, one of the most frequently diagnosed cancers in women. However, the mechanisms through which EDCs contribute to breast cancer development remain elusive. To rapidly proliferate, cancer cells undertake distinct metabolic programs to utilize existing nutrients in the tumor microenvironment and synthesize macromolecules de novo. EDCs are known to dysregulate cell signaling pathways related to cellular metabolism, which may be an important mechanism through which they exert their cancer‐promoting effects. These altered pathways can be studied via metabolomic analysis, a new advancement in ‐omics technologies that can interrogate molecular pathways that favor cancer development and progression. This review will summarize recent discoveries regarding EDCs and the metabolic reprogramming that they may induce to facilitate the development of breast cancer.

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