Dose-dependent effect of resveratrol on proliferation and apoptosis in endothelial and tumor cell cultures

Abstract

Experimental data suggest that Resveratrol, a compound found in grapes and other fruits may influence cell proliferation and apoptosis. The aim of our experiments was to study the effect of Resveratrol on tumor cell cultures and an endothelial cell culture in order to examine the effect of various doses of this compound on active cell death and cell proliferation. Human tumor (HT-29, SW-620, HT-1080) and endothelial (HUV-EC-C) cells were treated with various doses of (0.1 to 100.0 µg/ml) Resveratrol in vitro. Cell number, apoptotic and mitotic index was measured 24, 48 and 72 h after treatment. Low doses (0.1-1.0 µg/ml) of Resveratrol enhance cell proliferation, higher doses (10.0-100.0 µg/ml) induce apoptosis and decrease mitotic activity, which is reflected in changes of cell number. Resveratrol influences dose dependently the proliferative and apoptotic activity of human tumor and endothelial cells. The possible role of formaldehyde in the mechanism of action of Resveratrol is discussed.

Resveratrol displays converse dose-related effects on 5-fluorouracil-evoked colon cancer cell apoptosis: The roles of caspase-6 and p53

“…Many authors have reported a protective role of lower dose RSV against certain cellular insults, including oxidative stress-induced apoptosis by ethanol and hydrogen peroxide, as well as drug-induced apoptosis in cancer cells. [13][14][15] In particular, RSV has been shown to be antagonistic to paclitaxel-induced apoptosis in breast cancer and neuroblastoma cells at lower doses between 1-100 μM. 16 These findings are concordant with our results, which demonstrated that lower dose RSV protected against 5-FU-evoked apoptosis in colon cancer cells.…”

Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression

“…Based on our observation it was revealed that although resveratrol treatment at the concentrations of 0.25 and 0.5 μM produce optimum effect the higher concentration of this (5 μM) has detrimental impact. This is in line with the earlier reports of dose-dependent effect exerted by resveratrol in cell proliferation [20,39] as well as mammalian embryonic development [21,24]. Supplementation of antioxidants in culture medium influences oocyte maturation and embryonic development.…”

Resveratrol: From Basic Science to the Clinic

“…Studies on HUVEC (human umbilical vein endothelial cells) demonstrated that the molecule at low concentrations induces the proliferation of this cell phenotype, while, at high doses, it causes HUVEC growth impairment and apoptosis. 93 The inhibition of angiogenesis was also confirmed in several other cell systems and in a three-dimensional model of tube formation. 88 Investigation on the mechanism(s) by which the hydroxystilbene inhibits VEGF-induced angiogenesis showed that resveratrol effectively abrogated VEGF-mediated tyrosine phosphorylation of vascular endothelial-cadherin and its partner, b-catenin.…”