Dosage compensation of X-linked heat-shock puffs in Drophila pseudoobscura

Pierce, Dorothy A.; Lucchesi, John C.

doi:10.1007/bf00327265

Cited by 23 publications

(10 citation statements)

References 30 publications

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“…We first sought to confirm, by RNA probe protection, the earlier finding (22) by transcription autoradiography that the Hsp82 gene of D. pseudoobscura is dosage compensated. Fig.…”

Section: Resultsmentioning

confidence: 99%

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Dosage compensation of the Drosophila pseudoobscura Hsp82 gene and the Drosophila melanogaster Adh gene at ectopic sites in D. melanogaster.

Sass

Meselson

1991

Proc. Natl. Acad. Sci. U.S.A.

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Measurements were made of the amounts of larval RNA transcribed from the autosomal Adh gene of Drosophila melanogaster and the X chromosomal Hsp82 gene of Drosophilapseudoobscura carried on the same P-element transposon inserted at various sites in the D. melanogaster genome. Both genes were fully compensated at sites in euchromatic regions of the X chromosome but neither was compensated at a site in the centric 8-heterochromatin of the X chromosome. No compensation of the D. pseudoobscura Hsp82 gene was found at any of 10 autosomal insertion sites tested. The compensation behavior of the transposed genes was, therefore, not determined by closely linked sequences but instead was determined in each case by their new chromosomal environment.

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“…We first sought to confirm, by RNA probe protection, the earlier finding (22) by transcription autoradiography that the Hsp82 gene of D. pseudoobscura is dosage compensated. Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The Hsp82 gene is, therefore, autosomal in D. melanogaster and X chromosome-linked in D. pseudoobscura, where it has been shown by transcription autoradiography to be compensated (22).…”

Section: %mentioning

confidence: 99%

Dosage compensation of the Drosophila pseudoobscura Hsp82 gene and the Drosophila melanogaster Adh gene at ectopic sites in D. melanogaster.

Sass

Meselson

1991

Proc. Natl. Acad. Sci. U.S.A.

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“…For example, D. pseudobscura has a metacentrix X chromosome comprised of the X and 3L chromosomal elements of D. melanogaster. Enzyme (30) and heat shock (31) (32).…”

Section: Resultsmentioning

confidence: 99%

Autosomal dosage compensation Drosophila melanogaster strains trisomic for the left arm of chromosome 2.

Devlin¹,

Holm²,

Grigliatti³

1982

Proc. Natl. Acad. Sci. U.S.A.

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Drosophila melanogaster individuals trisomic for an entire chromosome arm can survive to late' stages of pupal development. We have examined the levels of five enzymes whose structural genes are located on the left arm ofchromosome 2 both in trisomy 2L and in diploid strains. In trisomies, three distally mapping loci showed compensated levels ofexpression close to that observed in the diploid strains. Analysis ofelectrophoretic variants of a compensated locus revealed that all three alleles are active in trisomies. Thai-two proximally located loci displayed dose-dependent levels of expression. Therefore, at the level of theindividual gene, autosomal compensation appears to bean all-or-nene phenomenon. Furthermore, the compensatory response may be regionally distributed along the chromosome arm. The presence of both autosomal and sex-linked dosage compensation prompts us to speculate that these phenomenon are similar homeostatic mechanisms that modulate gene expression both in euploid and aneuploid genomes.

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“…comm.) of the protocol of Pierce and Lucchesi (1980). Wandering-stage third-instar larvae were heatshocked for 20 min at 37°C.…”

Section: [3h]uridine Incorporationmentioning

confidence: 99%

A Drosophila rRNA gene located in euchromatin is active in transcription and nucleolus formation.

Karpen¹,

Schaefer²,

Laird³

1988

Genes & Development

159

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P-element transformants of a single rRNA gene (rDNA) were used to investigate the relationship between the organization of the nucleolus organizer (NO) and rDNA function in Drosophila melanogaster. In situ hybridization to rRNA in polytene nuclei of salivary glands demonstrated that an rRNA gene can be transcribed at a high rate when inserted into chromosomal sites other than the NO. Structures that resemble morphologically the endogenous nucleoli ('mininucleoli') were associated with four different euchromatic sites of rDNA insertion. Molecular analyses revealed that these mininucleoli contained both rRNA and an antigen specific to nucleoli. Phenotypes resulting from rDNA deficiencies were rescued partially by the presence of the transformed rDNA, indicating that the transcripts and mininucleoli associated with the rDNA insertion sites were functional. Thus, two conserved features of rDNA organization in eukaryotes, namely tandem repetition and heterochromatic localization, are not required for rRNA gene function. We conclude that 'nucleolar organizing activity' is an intrinsic property of the rDNA or its RNA products.

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Dosage compensation of X-linked heat-shock puffs in Drophila pseudoobscura

Cited by 23 publications

References 30 publications

Dosage compensation of the Drosophila pseudoobscura Hsp82 gene and the Drosophila melanogaster Adh gene at ectopic sites in D. melanogaster.

Dosage compensation of the Drosophila pseudoobscura Hsp82 gene and the Drosophila melanogaster Adh gene at ectopic sites in D. melanogaster.

Autosomal dosage compensation Drosophila melanogaster strains trisomic for the left arm of chromosome 2.

A Drosophila rRNA gene located in euchromatin is active in transcription and nucleolus formation.

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