Dopamine transporter immunohistochemistry in median eminence, amygdala, and other areas of the rat brain

Abstract

In an extension of our previous work, an antibody directed against a peptide from the N‐terminal region of DAT was used to localize specific dopamine transporter immunoreactivity (DAT‐IR) in several regions of rat brain. Apparent axons and varicosities were found in the zona incerta, external layer of the median eminence, various nuclei of the amygdala, the cortex‐amygdala transition zone, and in periglomerular regions in the olfactory bulb. Apparent stained neuronal perikarya and dendrites were observed in the arcuate nucleus and olfactory bulb. These regions are known to have dopaminergic neurons and innervations, although there was not a perfect correspondence between DAT‐IR and the known distribution of dopaminergic neurons. A possible explanation is that different dopamine containing cell groups express different levels of DAT mRNA and protein, as we have previously shown. Also in the tuberoinfundibular neurons, for example, DAT‐IR was preferentially localized to distal axons in the median eminence, suggesting intracellular compartmentation. (This article is a US Government work and, as such, is in the public domain in the United States of America.) © 1996 Wiley‐Liss, Inc.

The number of dopaminergic cells is increased in the olfactory bulb of monkeys chronically exposed to MPTP

“…The vast majority of the olfactory TH-ir cells co-expressed DDC and VMAT2 and none of the analyzed TH-ir cells expressed DAT, either in control animals or MPTP-monkeys (Table I). However, some cells of OB expressed DAT but not TH which is consistent with previous reports showing DAT expression in the rat OB (Revay et al, 1996) (Fig. 3).…”

Dopaminergic and cholinergic modulation of the amygdala is altered in female mice with oestrogen receptor β deprivation

“…1A and 2) immunoreactivity were comparable in ERβ −/− and ERβ +/+ mice, similar for the characteristics and distribution of DAT (Figs. 1B and 3), and they were consistent with previous results in the rodent amygdala 37,[68][69][70] . For example, as in the mouse and rat, the immunoreactivity of TH (Fig.…”

Neurochemical organization of the ventral striatum’s olfactory tubercle

“…The OT has robust DAT immunoreactivity, which is consistent with its midbrain DA fiber innervation (Ciliax et al, 1995;Freed et al, 1995;Revay, Vaughan, Grant, & Kuhar, 1996;Vastenhouw et al, 2007;Vaughan, Brown, McCoy, & Kuhar, 1996).…”

“…The DA transporter (DAT) is an integral membrane protein allowing for homeostasis of synaptic DA (Sonders, Zhu, Zahniser, Kavanaugh, & Amara, ; Torres, Gainetdinov, & Caron, ). The OT has robust DAT immunoreactivity, which is consistent with its midbrain DA fiber innervation (Ciliax et al, ; Freed et al, ; Revay, Vaughan, Grant, & Kuhar, ; Vastenhouw et al, ; Vaughan, Brown, McCoy, & Kuhar, ).…”