Dopamine-melanin nanoparticles scavenge reactive oxygen and nitrogen species and activate autophagy for osteoarthritis therapy

Zhong, Gang; Yang, Xueyuan; Jiang, Xianfang; Kumar, Anil; Long, Huiping; Xie, Jin; Zheng, Li; Zhao, Jinmin

doi:10.1039/c9nr03060c

Cited by 117 publications

(81 citation statements)

References 54 publications

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“…The phosphoinositide 3-kinase (PI3K) blocker 3-MA is routinely used as an inhibitor of autophagy 34 , and markedly abrogated the effects of PD on apoptosis and ROS generation. The expression levels of inflammation cytokines (IL-6, TNF- α, and MMP-13) and de-differentiation markers (Col I) were also significantly increased by treatment with 3-MA, this result is consistent with the findings of autophagy protection in existing researches 35–37 , one possible explanation is that induction of autophagy plays the key role in maintaining the phenotype of chondrocyte and inhibiting inflammatory response. In short, our findings reveal that PD likely relieves symptoms by activating autophagy, however, more studies are required to clarify the further mechanism.…”

Section: Discussionsupporting

confidence: 90%

RETRACTED ARTICLE: Chondro-protective effects of polydatin in osteoarthritis through its effect on restoring dysregulated autophagy via modulating MAPK, and PI3K/Akt signaling pathways

Luan

Zhang

et al. 2019

Sci Rep

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Osteoarthritis (OA) is a degenerative disease of the cartilage that is prevalent in the middle-aged and elderly demography. Polydatin (PD), a natural resveratrol glucoside, has shown significant anti-inflammatory and anti-arthritic potential in previous studies. This study was designed to evaluate the therapeutic properties of PD in vitro and in vivo, and elucidate their underlying mechanisms. The expression levels of all relevant factors were evaluated by qRT-PCR, western blotting, and immunohistochemistry (IHC) where suitable. Reactive oxygen species (ROS) and apoptosis were analyzed using the suitable probes and flow cytometry. The histological evidence of cartilage was assessed in rat models, moreover, the several serum cytokines levels and autophagy levels were evaluated. The result showed PD displayed significant chondro-protective effects, inferred in terms of reduced inflammation and cartilage degradation, apoptosis inhibition, and lower ROS production. The protective effects were attenuated by the autophagy inhibitor 3-MA, indicating a mediating role of autophagy in PD action. Mechanistically, PD exerted its effects by inhibiting the MAPK and PI3K/Akt signaling pathways which led to the down-regulation of mTOR. In conclusion, PD protects against cartilage degeneration by activating the autophagy flux in the chondrocytes via the MAPK and PI3K/Akt signaling pathways.

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Section: Discussionsupporting

confidence: 90%

RETRACTED ARTICLE: Chondro-protective effects of polydatin in osteoarthritis through its effect on restoring dysregulated autophagy via modulating MAPK, and PI3K/Akt signaling pathways

Luan

Zhang

et al. 2019

Sci Rep

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“…In comparison, in the treatment of rheumatoid arthritis (RA), fumagillin prodrug (Fum-PD) NPs can promote autophagic flux, followed by inhibition of the NF-κB signaling pathway-dependent inflammatory response [232]. Additionally, based on the significant effectiveness of anti-ROS therapy, dopamine melanin NPs were utilized to further decelerate cartilage degradation through autophagy activation for efficient treatment of osteoarthritis (OA) [233]. Subsequently, the targeted regulation of autophagy by using direct NMs or as drug carriers has introduce huge potential for clinical treatment of related diseases.…”

Section: Other Potential Therapiesmentioning

confidence: 99%

Nanomaterial-mediated autophagy: coexisting hazard and health benefits in biomedicine

Feng

Zhang

et al. 2020

Part Fibre Toxicol

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Background Widespread biomedical applications of nanomaterials (NMs) bring about increased human exposure risk due to their unique physicochemical properties. Autophagy, which is of great importance for regulating the physiological or pathological activities of the body, has been reported to play a key role in NM-driven biological effects both in vivo and in vitro. The coexisting hazard and health benefits of NM-mediated autophagy in biomedicine are nonnegligible and require our particular concerns. Main body We collected research on the toxic effects related to NM-mediated autophagy both in vivo and in vitro. Generally, NMs can be delivered into animal models through different administration routes, or internalized by cells through different uptake pathways, exerting varying degrees of damage in tissues, organs, cells, and organelles, eventually being deposited in or excreted from the body. In addition, other biological effects of NMs, such as oxidative stress, inflammation, necroptosis, pyroptosis, and ferroptosis, have been associated with autophagy and cooperate to regulate body activities. We therefore highlight that NM-mediated autophagy serves as a double-edged sword, which could be utilized in the treatment of certain diseases related to autophagy dysfunction, such as cancer, neurodegenerative disease, and cardiovascular disease. Challenges and suggestions for further investigations of NM-mediated autophagy are proposed with the purpose to improve their biosafety evaluation and facilitate their wide application. Databases such as PubMed and Web of Science were utilized to search for relevant literature, which included all published, Epub ahead of print, in-process, and non-indexed citations. Conclusion In this review, we focus on the dual effect of NM-mediated autophagy in the biomedical field. It has become a trend to use the benefits of NM-mediated autophagy to treat clinical diseases such as cancer and neurodegenerative diseases. Understanding the regulatory mechanism of NM-mediated autophagy in biomedicine is also helpful for reducing the toxic effects of NMs as much as possible.

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“…The production of ROS by dye 847-ZnO NPs was measured using NBT and DCFDA assay. The NBT assay is a colorimetric assay for ROS production in which water-soluble NBT salt is converted to water-insoluble, blue NBT-diformazan in the presence of ROS [65][66][67][68]. DCFDA is commonly used to detect ROS in cells by oxidizing the non-fluorescent molecule to fluorescent 2 ,7 -dichlorofluorescein (DCF); the amount of which is directly proportional to the ROS present [67][68][69].…”

Section: Production Of Rosmentioning

confidence: 99%

“…The NBT assay is a colorimetric assay for ROS production in which water-soluble NBT salt is converted to water-insoluble, blue NBT-diformazan in the presence of ROS [65][66][67][68]. DCFDA is commonly used to detect ROS in cells by oxidizing the non-fluorescent molecule to fluorescent 2 ,7 -dichlorofluorescein (DCF); the amount of which is directly proportional to the ROS present [67][68][69]. The light source used in this study to induce ROS production from the Dye 847-ZnO NPs was a NIR LED lamp (λ = 850 nm, Power density = 4.6 mW/cm 2 ).…”

Section: Production Of Rosmentioning

confidence: 99%

“…The release of ROS was partially negated by the presence of PEG in the NBT results. dichlorofluorescein (DCF); the amount of which is directly proportional to the ROS present [67][68][69]. The light source used in this study to induce ROS production from the Dye 847-ZnO NPs was a NIR LED lamp (λ = 850 nm, Power density = 4.6 mW/cm 2 ).…”

Section: Production Of Rosmentioning

confidence: 99%

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Near Infrared-Activated Dye-Linked ZnO Nanoparticles Release Reactive Oxygen Species for Potential Use in Photodynamic Therapy

Nagi

Skorenko²,

Bernier

et al. 2019

Materials

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Novel dye-linked zinc oxide nanoparticles (NPs) hold potential as photosensitizers for biomedical applications due to their excellent thermal- and photo-stability. The particles produced reactive oxygen species (ROS) upon irradiation with 850 nm near infrared (NIR) light in a concentration- and time-dependent manner. Upon irradiation, ROS detected in vitro in human umbilical vein endothelial cells (HUVEC) and human carcinoma MCF7 cells positively correlated with particle concentration and interestingly, ROS detected in MCF7 was higher than in HUVEC. Preferential cytotoxicity was also exhibited by the NPs as cell killing was higher in MCF7 than in HUVEC. In the absence of irradiation, dye-linked ZnO particles minimally affected the viability of cell (HUVEC) at low concentrations (<30 μg/mL), but viability significantly decreased at higher particle concentrations, suggesting a need for particle surface modification with poly (ethylene glycol) (PEG) for improved biocompatibility. The presence of PEG on particles after dialysis was indicated by an increase in size, an increase in zeta potential towards neutral, and spectroscopy results. Cell viability was improved in the absence of irradiation when cells were exposed to PEG-coated, dye-linked ZnO particles compared to non-surface modified particles. The present study shows that there is potential for biological application of dye-linked ZnO particles in photodynamic therapy.

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Dopamine-melanin nanoparticles scavenge reactive oxygen and nitrogen species and activate autophagy for osteoarthritis therapy

Abstract: Anti-oxidative agents hold great potential in osteoarthritis (OA) therapy.

Cited by 117 publications

References 54 publications

RETRACTED ARTICLE: Chondro-protective effects of polydatin in osteoarthritis through its effect on restoring dysregulated autophagy via modulating MAPK, and PI3K/Akt signaling pathways

RETRACTED ARTICLE: Chondro-protective effects of polydatin in osteoarthritis through its effect on restoring dysregulated autophagy via modulating MAPK, and PI3K/Akt signaling pathways

Nanomaterial-mediated autophagy: coexisting hazard and health benefits in biomedicine

Near Infrared-Activated Dye-Linked ZnO Nanoparticles Release Reactive Oxygen Species for Potential Use in Photodynamic Therapy

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