Dominant components of the <scp>T</scp>horoughbred metabolome characterised by <sup>1</sup><scp>H</scp>‐nuclear magnetic resonance spectroscopy: A metabolite atlas of common biofluids

Escalona, E. E.; Leng, Joy; Dona, Anthony C.; Merrifield, Claire A; Holmes, Elaine; Proudman, C. J.; Swann, Jonathan R.

doi:10.1111/evj.12333

Cited by 32 publications

(44 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the category of animal products, bovine-based studies had the most articles published (16 articles) relative to all other groups. Some of the more interesting applications of metabolomics found in our survey include the use of metabolomics for quality control of animal products [46; 47], evaluating nutritional value and impact of various feed sources on animal health and products [25], investigating disease biology by using animal models of human disease [48; 49], investigation of potential metabolite biomarkers of animal disease [22; 23], assessment of production traits [50; 51], reproductive performance [29], and general metabolome characterization [52; 53]. …”

Section: Resultsmentioning

confidence: 99%

“…Clark et al [89] reported 97 metabolites out of the 571 detected features in caprine serum using a combination of both GC-MS and LC-MS. In equine metabolomics, the highest number of metabolites identified was from a study conducted by Escalona and colleagues [53] with 102 metabolites identified via NMR analysis of plasma, urine and fecal water. A porcine metabolomics study by Metzler-Zebeli et al [90] reported 104 out of 132 detected serum metabolites using LC-MS.…”

Section: Resultsmentioning

confidence: 99%

“…Metabolomics is also well-positioned to provide some important advances in both livestock research and the livestock industry, especially as it relates to livestock health, breeding and production. A number of examples were highlighted in this review including metabolome discovery for normal metabolite composition and concentrations [52; 53], identification of biomarkers of transition diseases [21] as well as production traits in dairy [51] and beef cattle [20] with the goal of introducing prognostic strategies in animal health as well as increasing prediction accuracies. Our observations also showed that a wide variety of biofuids have received attention for metabolomics research such as metabolic profiling of milk, plasma, serum, and urine, minimizing animal welfare concerns.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Livestock metabolomics and the livestock metabolome: A systematic review

Goldansaz¹,

Guo²,

Sajed³

et al. 2017

PLoS ONE

259

247

View full text Add to dashboard Cite

Metabolomics uses advanced analytical chemistry techniques to comprehensively measure large numbers of small molecule metabolites in cells, tissues and biofluids. The ability to rapidly detect and quantify hundreds or even thousands of metabolites within a single sample is helping scientists paint a far more complete picture of system-wide metabolism and biology. Metabolomics is also allowing researchers to focus on measuring the end-products of complex, hard-to-decipher genetic, epigenetic and environmental interactions. As a result, metabolomics has become an increasingly popular “omics” approach to assist with the robust phenotypic characterization of humans, crop plants and model organisms. Indeed, metabolomics is now routinely used in biomedical, nutritional and crop research. It is also being increasingly used in livestock research and livestock monitoring. The purpose of this systematic review is to quantitatively and objectively summarize the current status of livestock metabolomics and to identify emerging trends, preferred technologies and important gaps in the field. In conducting this review we also critically assessed the applications of livestock metabolomics in key areas such as animal health assessment, disease diagnosis, bioproduct characterization and biomarker discovery for highly desirable economic traits (i.e., feed efficiency, growth potential and milk production). A secondary goal of this critical review was to compile data on the known composition of the livestock metabolome (for 5 of the most common livestock species namely cattle, sheep, goats, horses and pigs). These data have been made available through an open access, comprehensive livestock metabolome database (LMDB, available at http://www.lmdb.ca). The LMDB should enable livestock researchers and producers to conduct more targeted metabolomic studies and to identify where further metabolome coverage is needed.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Livestock metabolomics and the livestock metabolome: A systematic review

Goldansaz¹,

Guo²,

Sajed³

et al. 2017

PLoS ONE

259

247

View full text Add to dashboard Cite

show abstract

“…LEfSe (Segata et al, 2011) was used to identify differentially abundant OTUs. Bacterial metabolic profiles were characterized by 1 H NMR spectroscopy (Escalona et al, 2015), from urine, analysed by Principal Components Analysis. Fermentation of hay and oats, separately, were measured by in vitro gas production (Murray et al, 2006), data were analysed by repeated measures ANOVA.…”

Section: Objectivesmentioning

confidence: 99%

The Effect of Moxidectin Treatment on the Equine Hind Gut Microbiome, Metabonome and Feed Fermentation Kinetics in Horses with Very Low Parasite Burdens

2017

Equine Veterinary Education

View full text Add to dashboard Cite

. Background: Antimicrobial administration is a risk factor for gastrointestinal disease in horses. Dysbiosis, or changes in the gastrointestinal bacteria, may subsequently result in alterations of metabolic function.Objectives: The purpose of this study was to investigate the effects of metronidazole on the cecal and fecal microbiome and metabolome of horses.Methods: Metronidazole (15 mg/kg po q12 h) was administered to adult horses (n = 5) with indwelling cecal cannulas. Fecal and cecal microbiota and metabolite profiles were characterized by Illumina sequencing of 16 S rRNA genes and by an untargeted metabolomics approach using mass spectrometry platforms.Results: Metronidazole significantly decreased measures of alpha diversity in both fecal (Shannon, P = 0.021) and cecal samples (Chao1, P = 0.043; Observed OTUs, P = 0.001; Shannon, P = 0.002). Beta diversity, as measured by weighted Unifrac distances, showed significant decreases in both cecal and fecal samples (ANOSIM, P = 0.001). The bacterial taxa Christensenellaceae, Campylobacteriaceae, and Spirochaetaceae were significantly altered due to antibiotic administration. Of the 553 metabolites analyzed, 223 were named and 330 were unnamed. Analysis of the named fecal metabolites found 110 were significantly different after metronidazole administration (P < 0.05, q = 0.041). In contrast, only 2 cecal metabolites remained significant after adjustment for multiple comparisons (P < 0.05, q = 0.049) had been made. These metabolites were active in the biosynthesis of fatty acids, steroids, proteins, carbohydrates, sucrose, and starches.Conclusions: Metronidazole administration changed the cecal and fecal microbiome and metabolome in horses.Ethical animal research: Ethical approval obtained from the Animal Care and Use Committee at Texas A&M (AUP 2013-0123).

show abstract

“…Metabolomics is a powerful systems biological approach that simultaneously measures all metabolites present within biological fluids or tissues . Metabolite phenotypic profiling captures relationships of the cellular processes they represent.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic analysis of synovial fluid from Thoroughbred racehorses diagnosed with palmar osteochondral disease using magnetic resonance imaging

Graham

Anderson

Phelan

et al. 2019

Equine Veterinary Journal

View full text Add to dashboard Cite

Summary Background Palmar osteochondral disease (POD) is a common cause of lameness in competition horses. Magnetic resonance imaging (MRI) is the most sensitive diagnostic modality currently available, however it may not be financially or logistically practical for routine screening of POD. There is increasing interest in the use of metabolomics for diagnosis prior to progression to irreversible damage. Objectives To determine metabolite levels in synovial fluid (SF) of horses with a clinical diagnosis of POD based on diagnostic analgesia and MRI, with the hypothesis that metabolomic profiles differ between diseased and healthy joints. Study design Prospective clinical study. Methods Synovial fluid was collected from metacarpo/tarsophalangeal joints (MC/TPJ) of 29 horses (n = 51 joints), including 14 controls (n = 26) and 15 cases (n = 25), the latter with lameness localised to the MC/TPJ and MR changes consistent with POD (n = 23). Spectra were produced using 1H‐nuclear magnetic resonance (NMR) spectroscopy and analysed. Results Twenty‐five metabolites were recognised associated with various biosynthetic and degradation pathways. The metabolite abundances within the controls demonstrated increased variability compared with the clinical group. The low level of variance between the spectra of the two groups was explained by five principal components. Cross‐validation of the cohort demonstrated modest separation of predictive power (R2 = 0.67; Q2 = 0.34). Although statistical significance was not achieved, the most influential metabolites were glucose and lactate. Main limitations The modest sample size and variation in signalment, background and presenting condition of the controls may have impacted the discriminative power of the constructed models. The lack of matched controls, differences in time of fluid collection and freezing times may have also reduced accuracy when representing metabolite profiles. Conclusions This study identified and quantified metabolites present in MC/TPJ SF of clinical cases with POD.

show abstract

Dominant components of the Thoroughbred metabolome characterised by ¹H‐nuclear magnetic resonance spectroscopy: A metabolite atlas of common biofluids

Cited by 32 publications

References 58 publications

Livestock metabolomics and the livestock metabolome: A systematic review

Livestock metabolomics and the livestock metabolome: A systematic review

The Effect of Moxidectin Treatment on the Equine Hind Gut Microbiome, Metabonome and Feed Fermentation Kinetics in Horses with Very Low Parasite Burdens

Metabolomic analysis of synovial fluid from Thoroughbred racehorses diagnosed with palmar osteochondral disease using magnetic resonance imaging

Contact Info

Product

Resources

About

Dominant components of the Thoroughbred metabolome characterised by 1H‐nuclear magnetic resonance spectroscopy: A metabolite atlas of common biofluids

Cited by 32 publications

References 58 publications

Livestock metabolomics and the livestock metabolome: A systematic review

Livestock metabolomics and the livestock metabolome: A systematic review

The Effect of Moxidectin Treatment on the Equine Hind Gut Microbiome, Metabonome and Feed Fermentation Kinetics in Horses with Very Low Parasite Burdens

Metabolomic analysis of synovial fluid from Thoroughbred racehorses diagnosed with palmar osteochondral disease using magnetic resonance imaging

Contact Info

Product

Resources

About

Dominant components of the Thoroughbred metabolome characterised by ¹H‐nuclear magnetic resonance spectroscopy: A metabolite atlas of common biofluids