2021
DOI: 10.3390/pathogens10060634
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Abstract: In a previous work, we demonstrated that nasally administered Dolosigranulum pigrum 040417 beneficially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 3 (TLR3) and improved protection against Respiratory Syncytial Virus (RSV) in mice. In this work, we aimed to evaluate the immunomodulatory effects of D. pigrum 040417 in human respiratory epithelial cells and the potential ability of this immunobiotic bacterium to increase the protection against Acute Respirat… Show more

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Cited by 10 publications
(21 citation statements)
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“…The FBS was reduced to 2% in antiviral assays. The cells were incubated at 37 °C in a humidified incubator in an atmosphere with 5% CO 2 as described previously [ 25 ].…”
Section: Methodsmentioning
confidence: 99%
See 3 more Smart Citations
“…The FBS was reduced to 2% in antiviral assays. The cells were incubated at 37 °C in a humidified incubator in an atmosphere with 5% CO 2 as described previously [ 25 ].…”
Section: Methodsmentioning
confidence: 99%
“…Cells were further incubated for 24 h at 37 °C, 5% CO 2 . Lactobacilli were removed by washing with PBS and the Calu-3 cells were stimulated with 15 μg/mL of poly(I:C) (Sigma-Aldrich, San Luis, MO, USA), with a synthetic double-stranded RNA (dsRNA) and TLR3 agonist [ 25 ]. Before the challenge with poly(I:C) (basal levels) and 48 h after TLR3 activation, culture supernatants were collected for the evaluation of cytokines by enzyme-linked immunosorbent assay (ELISA) technique kits as described previously [ 25 ].…”
Section: Methodsmentioning
confidence: 99%
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“…Further experiments with D. pigrum showed this effect was associated with reduced lung damage markers and increased resistance to RSV infection (Ortiz Moyano et al, 2020). The same strain of D. pigrum was also found to increase expression of IFNβ as well as IL-6 in a bronchial epithelial cell line (Calu-3) which was associated with reduced viral titres of SARS-CoV-2 and a reduction in cell cytotoxicity (Islam et al, 2021).…”
Section: Dolosigranulum Pigrum and Corynebacterium Sppmentioning
confidence: 84%