Does uranium exposure induce genotoxicity in the teleostean Danio rerio? First experimental results

Barillet, Sabrina; Buet, Astrid; Adam, Christelle; Devaux, A.

doi:10.1051/radiopro:2005s1-028

Cited by 31 publications

(25 citation statements)

References 9 publications

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“…But when we adjust the deb model with the one compartment toxico-kinetic module to whole body residues provided in Barillet et al (2005) and Barillet et al (2011), we see that at 2100 nM U, uptake should be very important but it hardly is compared to predictions ( fig. 8C).…”

Section: Adult Whole Body Residuesmentioning

confidence: 99%

“…The values of toxicity parameters used to obtain the fits provided for each of the exposed females in this study can be found in ], then the individuals in the 37d-U420 and 15d-1054 as well as in the higher exposure conditions by Barillet et al (2005Barillet et al ( , 2011 would have shrunk to death during the experiment. This is manifestly not the case.…”

Section: Mode Of Action Of Uranium On Females In Reproduction Trialsmentioning

confidence: 99%

“…Barillet et al (2005) and Barillet et al (2011) present adult bioaccumulation data (nmol U per g whole body wet mass: M Q w ) at several exposure concentrations (table A.1, Online Appendix A). Bourrachot et al (2008) demonstrate toxicological effects of U on embryo and early juvenile zebrafish growth and survival in combination with bioaccumulation.…”

Section: Other Data and Parameter Estimationmentioning

confidence: 99%

“…At each sampling time 10 random individuals were sampled and biometry and M Q w was recorded. In the study by Barillet et al (2005) fish of both sexes were sampled, while in the study by Barillet et al (2011) only males were sampled. We consider that males and females both possess the same model parameters (table 1).…”

Section: D Adult Accumulation Experimentsmentioning

confidence: 99%

“…We calibrated initial conditions to initial observed mean W w in each condition in Barillet et al (2005) and to initial observed mean W w and L f in Barillet et al (2011). Consequently, there are three free parameters for each condition in Barillet et al (2005):…”

Section: D Adult Accumulation Experimentsmentioning

confidence: 99%

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Effects of uranium on the metabolism of zebrafish, Danio rerio

et al. 2012

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The increasing demand for nuclear energy results in heightened levels of uranium (U) in aquatic systems which present a potential health hazard to resident organisms. The aim of this study was to mechanistically assess how chronic exposure to environmentally relevant concentrations of U perturbs the complex interplay between feeding, growth, maintenance, maturation and reproduction throughout the life-cycle of an individual. To this end we analysed literature-based and original zebrafish toxicity data within a same mass and energy balancing conceptual framework. U was found to increase somatic maintenance leading to inhibition of spawning as well as increase hazard rate and costs for growth during the early life stages. The fish's initial conditions and elimination through reproduction greatly affected toxico-kinetics and effects. We demonstrate that growth and reproduction should be measured on specific individuals since mean values were hardly interpretable. The mean food level differed between experiments, conditions and individuals. This last 'detail' contributed substantially to the observed variability by its combined effect on metabolism, toxic effects and toxico-kinetics. The significance of this work is that we address exactly how these issues are related and derive conclusions which are independent of experimental protocol and coherent with a very large body of literature on zebrafish eco-physiology.

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Section: Adult Whole Body Residuesmentioning

confidence: 99%

Section: Mode Of Action Of Uranium On Females In Reproduction Trialsmentioning

confidence: 99%

Section: Other Data and Parameter Estimationmentioning

confidence: 99%

Section: D Adult Accumulation Experimentsmentioning

confidence: 99%

Section: D Adult Accumulation Experimentsmentioning

confidence: 99%

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Effects of uranium on the metabolism of zebrafish, Danio rerio

et al. 2012

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Genetic, Biochemical, and Individual Responses of the Teleost Fish Carassius auratus to Uranium

Lourenço

Castro

Machado

et al. 2009

Arch Environ Contam Toxicol

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Carassius auratus were exposed for 96 h to different concentrations of uranyl nitrate (corresponding to 0, 100, 450, and 2,025 microg U L(-1)) and killed after different postexposure periods (0, 48, and 96 h) to assess uranium bioaccumulation, peroxisome proliferation (catalase [CAT]), lipid peroxidation (thiobarbituric acid reactive substances [TBARS]), and DNA integrity in erythrocytes (comet assay). In addition, feeding behaviour was recorded as a general response to toxicant exposure. Results provided evidence of uranium bioaccumulation in muscle of C. auratus after exposure to the highest concentrations (450 and 2,025 microg U L(-1)). This tissue was able to depurate uranium to control levels 96 h after exposure ceased. However, no perturbations in feeding behaviour or cell damage were observed in the tested organisms, except for the apparent irreversible inhibition of CAT activity immediately after exposure in the highest concentration tested. Data on DNA integrity (comets) showed that waterborne uranium exposure was able to induce genotoxicity in C. auratus erythrocytes because fish exposed to all concentrations exhibited higher DNA damage than controls 96 h after exposure. No DNA damage repair was apparent throughout the postexposure period, which was contrary to a recovery scenario. This experiment provides evidence of uranium's ability to induce physiologic impairment and genotoxicity in freshwater fish at environmentally relevant concentrations.

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Transfer modelling and toxicity evaluation of the effluent from an installation of cleansing and uranium recovery using a battery of bioassays

et al. 2010

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On July 7, 2008, a leak of effluent from an Installation of Cleansing and Uranium Recovery (Tricastin, France) led to the spillage of uranium in a stream. The acute toxicity of the effluent was evaluated, and compared to the toxicity of uranium nitrate in bioassays using several organisms: Chlamydomonas reinhardtii, Daphnia magna, Chironomus riparius and Danio rerio. A sediment bioassay was also performed on C. riparius using water and sediment sampled along the river. Results showed that effluent EC(50) 72 h was 0.65 mg U/l for algae and LC(50) 48 h was 1.67 mg U/l for daphnia, while values obtained for uranium nitrate were higher. The LC(50) 96 h of effluent to C. riparius was 22.7 mg U/l, similar to value for uranium nitrate; the sediment collected was not toxic to C. riparius larvae. The LOEC of effluent and uranium nitrate on HT(50) of D. rerio were similar (0.03 mg U/l), but larvae were more sensitive to uranium nitrate than to effluent. Our results suggest that other substances contained in the effluent could potentially be toxic to wildlife in association with uranium. In parallel, the modelling of the transfers based on uranium measurements in the surface water was used to fill data gaps and assess the impact along the river. These results provided an estimate of exposure conditions that occurred along the river. This approach allowed us to see that the risk to ecosystem during this incident was certainly low and concerned a short period of time, but it could have existed at least for some species.

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Does uranium exposure induce genotoxicity in the teleostean Danio rerio? First experimental results

Cited by 31 publications

References 9 publications

Effects of uranium on the metabolism of zebrafish, Danio rerio

Effects of uranium on the metabolism of zebrafish, Danio rerio

Genetic, Biochemical, and Individual Responses of the Teleost Fish Carassius auratus to Uranium

Transfer modelling and toxicity evaluation of the effluent from an installation of cleansing and uranium recovery using a battery of bioassays

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