Does SARS-CoV-2 reach peritoneal effluent?

Sadioğlu, Rezzan Eren; Aktar, Merve; Gülten, Ezgi; Eyupoglu, Sahin; Karahan, Zeynep Ceren; Azap, Alpay; Şengül, Şule; Ateş, Kenan; Kutlay, Sim

doi:10.1177/0896860820947331

Cited by 12 publications

(10 citation statements)

References 3 publications

(3 reference statements)

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“…In our patients, COVID-19 was diagnosed by RT-PCR of nasal swab samples. The findings corroborate previous results in 4 chronic PD patients reported by other groups 7,8 . To the best of our knowledge, the report by Only a small fraction of patients that tested positive for SARS-CoV-2 are viremic, and those are typically patients with severe COVID-19 symptoms [1][2][3][4] .…”

Section: Discussionsupporting

confidence: 93%

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SARS-CoV-2 in Spent Dialysate from Chronic Peritoneal Dialysis Patients with COVID-19

et al. 2021

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Background To date it is unclear whether SARS-CoV-2 is present in spent dialysate from peritoneal dialysis (PD) patients with COVID-19. Our aim was to assess the presence or absence of SARS-CoV-2 in spent dialysate from chronic PD patients with confirmed diagnosis of COVID-19. Methods Spent PD dialysate samples from COVID-19 positive PD patients were collected between March and August 2020. The multiplexed real-time reverse transcriptase-polymerase chain reaction assay contained primer/probe sets specific to different SARS-CoV-2 genomic regions and to bacteriophage MS2 as internal process control for nucleic acid extraction. Demographic and clinical data were obtained from patients' electronic health records. Results A total of 26 spent PD dialysate samples were collected from 11 patients from 10 dialysis centers. Spent PD dialysate samples were collected on average 25±13 days (median 20, range 10 to 45) after onset of symptoms. The temporal distance of PD effluent collection relative to the closest positive nasal swab RT PCR was 15±11 days (median 14; range 1 to 41). All 26 PD effluent samples tested negative at three SARS-CoV-2 genomic regions. Conclusions Our findings indicate the absence of SARS-CoV-2 in spent PD dialysate collected 10 days or later after the onset of COVID-19 symptoms. We cannot rule out presence of SARS-CoV-2 in spent PD dialysate in the early stage of COVID-19.

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Section: Discussionsupporting

confidence: 93%

“…Data on the presence or absence of SARS-CoV-2 in spent PD dialysate are scarce; to date results from only 5 chronic PD patients have been reported. SARS-CoV-2 was found to be absent in 4 patients 7,8 and present in one 9 .…”

Section: Introductionmentioning

confidence: 90%

SARS-CoV-2 in Spent Dialysate from Chronic Peritoneal Dialysis Patients with COVID-19

et al. 2021

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“…Seeliger B et al, (26) and Safari S. et al, (27) did not identify intraperitoneal viral RNA in patients who underwent surgical procedures in 5 and 4 cases, respectively. Similar observations were made in small groups of patients treated with acute peritoneal dialyses (28,29,30) and in several case studies (31,32,33). We encountered 3 case reports indicating the presence of SARS-CoV-2 virus in ascites due to decompensated cirrhosis (34,35) and bowel obstruction (36).…”

Section: Discussionsupporting

confidence: 80%

Absence of SARS-CoV-2 RNA in Peritoneal Fluid During Surgery in Pregnant Women Who Are COVID-19 Positive

Jakimiuk¹,

Januszewski²,

Santor-Zaczynska³

et al. 2021

Journal of Minimally Invasive Gynecology

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Study Objective: COVID-19 infection poses significant risks during surgical interventions. We investigated the intraperitoneal presence of SARS-CoV-2 in COVID-19 positive patients. Design: A prospective group study. Setting: Department of Obstetrics and Gynecology designated for COVID-19 patients, Central Clinical Hospital of the Ministry of Interior, Warsaw Patients: Overall, 65 pregnant women with COVID-19 infection underwent caesarian section. The diagnosis was confirmed either by positive antigen test or by positive RT-PCR assay performed within no more than 13 days prior to the operation. Interventions: On the day of the operation, a nasopharyngeal swab was taken, and peritoneal fluid was collected at the beginning of the operation. Both the nasopharyngeal swab and peritoneal fluid samples were tested for SARS-CoV-2. Measurements and Main Results: A total of 65 pregnant women with COVID-19 infection were enrolled in the study. The SARS-CoV-2 RNA test by nasopharyngeal swab produced positive results in 34 patients. In this group as well as in 31 non-confirmed patients all peritoneal fluid samples tested negative for SARS-CoV-2 RNA. Conclusion: These results suggest a low risk of COVID-19 transmission from the peritoneal cavity at the time of laparoscopy or laparotomy

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“…1); 5 were females and 3 males, while the mean age was 78 years (range 44-92 years); 2 patients were operated during the rst wave of the pandemic, 2 more during the second wave, and 4 during the third wave. The following surgical procedures were performed: In a recent article, some Authors performed SARS-CoV-2 RT-PCR assay from the peritoneal e uent after dialysis of a symptomatic COVID-19 patient: the assay was performed twice after 6-h duration of dwells without centrifugation, but it resulted negative in both samples (22).…”

Section: Resultsmentioning

confidence: 99%

Swab test in biological fluids as predictor of COVID-19 transmission risk during surgery: A prospective cross-sectional study from an Italian COVID center

Fabbri

Pesce

Ussia

et al. 2021

Preprint

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Background. The contamination of body fluids by Severe Acute Respiratory Syndrome Coronavirus 2 during surgery is current matter of debate in the scientific literature concerning CoronaVIrus Disease 2019. Surgical guidelines were published during the first wave of the COVID-19 pandemic and recommended to avoid laparoscopic surgery as much as possible, in fear that the chimney effect of high flow intraperitoneal gas escape during, and after, the procedure would increase the risk of viral transmission.Aim. The aim of this study was to evaluate the possibility of SARS-CoV-2 transmission during surgery by searching for viral RNA in serial samplings of biological liquids.Methods. This is a single center prospective cross-sectional study. We used a real-time reverse transcriptase (RT) polymerase chain reaction (PCR) test to perform swab tests for the qualitative detection of nucleic acid from SARS-CoV-2 in abdominal fluids, during emergency surgery and on the first post-operative day. In the case of thoracic surgery, we performed a swab test of pleural fluids during chest drainage placement as well as on the first post-operative day. Results. A total of 20 samples were obtained: 5 from pleural fluids, 13 from peritoneal fluids and two from biliary fluid. All 20 swabs performed from biological fluids resulted negative for SARS-CoV-2 RNA detection.Conclusion. To date, there is no scientific evidence of possible contagion by laparoscopic aerosolization of SARS-CoV-2, neither is certain whether the virus is effectively present in biological fluids.

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Does SARS-CoV-2 reach peritoneal effluent?

Cited by 12 publications

References 3 publications

SARS-CoV-2 in Spent Dialysate from Chronic Peritoneal Dialysis Patients with COVID-19

SARS-CoV-2 in Spent Dialysate from Chronic Peritoneal Dialysis Patients with COVID-19

Absence of SARS-CoV-2 RNA in Peritoneal Fluid During Surgery in Pregnant Women Who Are COVID-19 Positive

Swab test in biological fluids as predictor of COVID-19 transmission risk during surgery: A prospective cross-sectional study from an Italian COVID center

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