Dodartia orientalis L. essential oil exerts antibacterial activity by mechanisms of disrupting cell structure and resisting biofilm

Wang, Fei; Wei, Fu-Yao; Song, Chaoyun; Jiang, Bin; Tian, Shangyi; Yi, Jingwen; Yu, Chunlei; Song, Zhenbo; Sun, Luguo; Bao, Yongli; Wu, Yin; Huang, Yanxin; Li, Yuxin

doi:10.1016/j.indcrop.2017.08.058

Cited by 100 publications

(46 citation statements)

References 48 publications

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“…Wang et al . () have reported that Dodartia orientalis L. essential oil contained 13·83% α‐terpineol, which is similar to the α‐terpineol content of MAO that we have observed.…”

Section: Discussionsupporting

confidence: 88%

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Antifungal mechanisms of α‐terpineol and terpene‐4‐alcohol as the critical components of Melaleuca alternifolia oil in the inhibition of rot disease caused by Aspergillus ochraceus in postharvest grapes

Kong

Zhang

et al. 2019

J Appl Microbiol

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Aims: As a natural antimicrobial agent, Melaleuca alternifolia oil (MAO) is generally recognized to be safe and effective in the inhibition of phytopathogenic fungi. Due to lack of comprehensive studies on MAO for controlling postharvest Aspergillus, we investigated the preservative mechanism of MAO and its components against Aspergillus ochraceus in postharvest grapes to evaluate their potential effectiveness as fruit preservatives. Methods and Results: In our study, the compositions in MAO were analysed by gas chromatography-mass spectrometry. The inhibitory effects of MAO and its main constituents against A. ochraceus were compared by scanning electron microscopy and transmission electron microscopy observation, and metabolic analysis. Two components of MAO, a-terpineol and terpene-4-alcohol, showed higher antifungal effects than MAO, of which a-terpineol caused the worst leakage of cytoplasm and most serious hyphae distortions and spore disruptions. The downregulation of metabolic pathways of A. ochraceus was strongest with a-terpineol. The best inhibitory efficacy against A. ochraceus in grapes also occurred with a-terpineol. 3-Carene showed little inhibitory effect. Conclusions: These results demonstrate that not all components in MAO possess antimicrobial effects, and a-terpineol is the main contributor of MAO's A. ochraceus inhibition effect. Significance and Impact of the Study: a-Terpineol may be used as an alternative natural preservative for the postharvest storage of grapes and other fruits.

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“…Wang et al . () have reported that Dodartia orientalis L. essential oil contained 13·83% α‐terpineol, which is similar to the α‐terpineol content of MAO that we have observed.…”

Section: Discussionsupporting

confidence: 88%

“…Essential oils extracted from plants not only inhibit a variety of pathogens, but also are harmless to the human at the appropriate dosage (Wang et al . ). Essential oils have been widely used due to their antifungal, bactericidal, virucidal, anti‐inflammatory and antioxidative properties (Merghni et al .…”

Section: Introductionmentioning

confidence: 97%

Antifungal mechanisms of α‐terpineol and terpene‐4‐alcohol as the critical components of Melaleuca alternifolia oil in the inhibition of rot disease caused by Aspergillus ochraceus in postharvest grapes

Kong

Zhang

et al. 2019

J Appl Microbiol

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“…When the cell wall is destroyed, AKP will leak out, which can be detected at a wave length of 405 nm. Therefore, AKP is an indicator of the completeness of the bacterial cell wall (Wang et al, 2017). and membrane permeability (Bos & Tommassen, 2004).…”

Section: Cell Wall Damagementioning

confidence: 99%

Chemical composition, antibacterial activity, and mechanism of action of essential oil from Litsea cubeba against foodborne bacteria

Lin

Feng

et al. 2020

J Food Process Preserv

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Litsea cubeba (LC) is widely used as a food flavoring. In this study, 30 components of the essential oil of Litsea cubeba (LCEO) were identified, of which α‐citral (33.6534%) and β‐citral (27.8042%) were identified as the main components. The diameter of the inhibition zone, minimum inhibitory concentration, and minimum bactericidal concentration of LCEO to several foodborne bacteria were evaluated. The results indicated that gram‐negative bacteria (Salmonella, E. coli O157, E. coli DH5α, E. coli O104) were obviously sensitive to LCEO with Salmonella and E. coli O157 having the greatest sensitivity. According to the experimental results of the alkaline phosphatase assay, nucleic acid assay, propidium iodide staining, and electron microscopy observation, the mechanism of antibacterial effect of LCEO against Salmonella and E. coli O157 might involve the integrity of the cell membrane and wall. The results indicate that LCEO would be suitable as an effective food preservative. Practical applications In food industry, the essential oil of Litsea cubeba (LCEO) can serve as a natural preservative for fruits and vegetables, especially for foods vulnerable to gram‐negative bacteria. For example, berries are likely to be attacked by Salmonella and their preservation had long been a trouble. Through spraying or soaking of LCEO, the shelf life of berries can be largely prolonged. LCEO can also be applied to animal‐derived products. Due to its environmentally friendly feature, LCEO is a reassuring preservative. Otherwise, LCEO has good aroma because of large component of citral; therefore, it is also a great food flavoring.

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“…Determination of MIC and Minimum Bactericidal Concentrations. Minimum inhibitory concentrations and minimum bactericidal concentrations (MBC) were assessed using the microdilution method with some modifications (Wang et al, 2017). Briefly, LMEO was thoroughly dissolved in absolute ethanol to obtain serial dilutions in concentrations ranging from 1 to 100%.…”

Section: Determination Of Bacterial Inhibition Zonesmentioning

confidence: 99%

“…The increased AKP activity confirmed that LMEO9 damaged the bacterial cell wall. Therefore, LMEO9 exerts its bactericidal effect through a loss of cell wall integrity, allowing microbes and hydrolytic enzymes to enter cells, inducing cell death (Wang et al, 2017).…”

Section: Cell Wall Damage Evaluationmentioning

confidence: 99%

A natural biopreservative: Antibacterial action and mechanisms of Chinese Litsea mollis Hemsl. extract against Escherichia coli DH5α and Salmonella spp.

Cai

Wang

Chen

et al. 2019

Journal of Dairy Science

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Chemical preservatives have potential safety hazards, which may pose threats to human health. Safer biopreservatives are therefore urgently required. This study investigated the bacteriostatic activity and mechanism of Litsea mollis Hemsl. essential oil against Escherichia coli DH5α and Salmonella spp. Antibacterial activity of Litsea mollis Hemsl. essential oil 9 (LMEO9) against E. coli DH5α was observed (zone of inhibition was 5.0 ± 0.2 mm; minimum inhibitory concentration was 0.05%). Increases in electrolyte, nucleic acid, and alkaline phosphatase leakage in LMEO9-treated bacteria suggested that the cell envelope had been damaged. Scanning and transmission electron microscopy also demonstrated morphological alterations and content leakage during LMEO9 treatment. According to the kill-time analysis and propidium iodide uptake assay, LMEO9 led to cell death. These results demonstrated that LMEO9, which could affect bacterial cell envelope structural integrity, is a low-cost biopreservative that could be useful for the dairy industry and in fresh storage.

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Dodartia orientalis L. essential oil exerts antibacterial activity by mechanisms of disrupting cell structure and resisting biofilm

Cited by 100 publications

References 48 publications

Antifungal mechanisms of α‐terpineol and terpene‐4‐alcohol as the critical components of Melaleuca alternifolia oil in the inhibition of rot disease caused by Aspergillus ochraceus in postharvest grapes

Antifungal mechanisms of α‐terpineol and terpene‐4‐alcohol as the critical components of Melaleuca alternifolia oil in the inhibition of rot disease caused by Aspergillus ochraceus in postharvest grapes

Chemical composition, antibacterial activity, and mechanism of action of essential oil from Litsea cubeba against foodborne bacteria

A natural biopreservative: Antibacterial action and mechanisms of Chinese Litsea mollis Hemsl. extract against Escherichia coli DH5α and Salmonella spp.

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