Docosahexaenoic acid attenuates LPS-stimulated inflammatory response by regulating the PPARγ/NF-κB pathways in primary bovine mammary epithelial cells

He, Xuexiu; Liu, Weijian; Shi, Mingyu; Yang, Zhengtao; Zhang, Xichen; Gong, Pengtao

doi:10.1016/j.rvsc.2016.12.011

Cited by 56 publications

(36 citation statements)

References 32 publications

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“…Thus, inhibiting NF-B may be therapeutically significant approach. DHA has been shown to inhibit lipopolysaccharide-induced activation of NF-B in mammary epithelial cells [34], which supports our present findings that DHA reduces NF-B expression in pancreatic tissues of cerulein-treated mice. It is well known that nuclear translocation of NF-B is essential to determine its activation.…”

Section: Discussionsupporting

confidence: 92%

Docosahexaenoic Acid Inhibits Expression of Fibrotic Mediators in Mice With Chronic Pancreatitis

et al. 2019

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Background: Chronic pancreatitis (CP) is an irreversible progressive disease that destroys exocrine parenchyma, which are replaced by fibrous tissue. As pancreatic fibrosis is a key feature of CP, reducing fibrotic protein content in the pancreas is crucial for preventing CP. Studies suggest that NF-B facilitates the expression of fibrotic mediators in pancreas and protein kinase C- (PKC-) regulates NF-B activation in stimulated pancreatic acinar cells. Docosahexaenoic acid (DHA) is an omega-3 fatty acid having anti-inflammatory and anti-fibrotic effects. It has been shown to inhibit NF-B activity in cerulein-stimulated pancreatic acinar cells which is a cellular model of CP. In the present study, we investigated if DHA inhibits expression of fibrotic mediators by reducing PKC- and NF-B expression in mouse pancreatic tissues with CP. Methods: For six weeks, mice were weekly induced for acute pancreatitis to develop CP. Furthermore, acute pancreatitis was induced by hourly intraperitoneal injections of cerulein (50 g/kg × 7). Mice were administered DHA (10 M) via drinking water before and after CP induction. Results: Cerulein-induced pancreatic damages like decreased pancreatic weight/total body weight, leukocyte infiltration, necrosis of acinar cells, and vacuolization were found to be inhibited by DHA. Additionally, DHA inhibited cerulein-induced fibrotic mediators like alpha-smooth muscle actin and fibronectin in pancreas. DHA reduced expression of PKC- and NF-B p65 in pancreatic tissues of cerulein-treated mice. Conclusions: DHA may be beneficial in preventing CP by suppressing pancreatic expression of fibrotic mediators.

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Section: Discussionsupporting

confidence: 92%

Docosahexaenoic Acid Inhibits Expression of Fibrotic Mediators in Mice With Chronic Pancreatitis

et al. 2019

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“…Therefore, DHA and EPA differently modulate immune responses related to phagocytosis, phosphorylation of intracellular signaling molecules, activation of transcription factors, and effector immune-related gene expression; which largely depends of the cell type or target species. The overall modulatory effects of DHA and EPA are related to a polarized anti-inflammatory response demonstrated by the reduction of cytokines such as TNF-α, IFN-γ, IL-1β, IL-6, IL-12, and the anti-inflammatory cytokine IL-10, through the inhibition of kinases (i.e., JNK, ERK) of the NFκβ pathway (Xue et al, 2006 ; Weldon et al, 2007 ; Draper et al, 2011 ; Magee et al, 2012 ; Siriwardhana et al, 2012 ; Tsunoda et al, 2015 ; He et al, 2017 ; Dawczynski et al, 2018 ). These effects could be associated with the eicosanoid synthesis pathway (Lokesh et al, 1988 ).…”

Section: Relevant Characteristics Of Schizochytrium Sp mentioning

confidence: 99%

Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Ramos‐Vega¹,

Rosales‐Mendoza

Bañuelos-Hernández

et al. 2018

Front. Microbiol.

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Although oral subunit vaccines are highly relevant in the fight against widespread diseases, their high cost, safety and proper immunogenicity are attributes that have yet to be addressed in many cases and thus these limitations should be considered in the development of new oral vaccines. Prominent examples of new platforms proposed to address these limitations are plant cells and microalgae. Schizochytrium sp. constitutes an attractive expression host for vaccine production because of its high biosynthetic capacity, fast growth in low cost culture media, and the availability of processes for industrial scale production. In addition, whole Schizochytrium sp. cells may serve as delivery vectors; especially for oral vaccines since Schizochytrium sp. is safe for oral consumption, produces immunomodulatory compounds, and may provide bioencapsulation to the antigen, thus increasing its bioavailability. Remarkably, Schizochytrium sp. was recently used for the production of a highly immunoprotective influenza vaccine. Moreover, an efficient method for transient expression of antigens based on viral vectors and Schizochytrium sp. as host has been recently developed. In this review, the potential of Schizochytrium sp. in vaccinology is placed in perspective, with emphasis on its use as an attractive oral vaccination vehicle.

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“…In addition, accumulating evidence reveals that activation of the PPARs are involved in the various types of inflammatory processes, due to the inhibition of pro-inflammatory genes expression and negative regulation of pro-inflammatory transcription factor signaling pathways, in inflammatory cells [ 5 ]. Furthermore, activation of PPARs shows the anti-inflammatory effect by inhibiting the activation of nuclear factor-κB (NF-κB), leading to a decrease of pro-inflammatory cytokines and mediators [ 6 ]. Therefore, PPARs have been shown to be the drug targets to treat various related inflammatory diseases, such as vascular diseases, cancer, and neurodegenerative diseases [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Nuciferine Inhibits Proinflammatory Cytokines via the PPARs in LPS-Induced RAW264.7 Cells

et al. 2018

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Inflammation is important and has been found to be an underlying cause in many acute and chronic human diseases. Nuciferine, a natural alkaloid containing an aromatic ring, is found in the nelumbo nucifera leaves. It has been shown to have potential anti-inflammatory activities, but the molecular mechanism has remained unclear. In this study, we found that nuciferine (10 μM) significantly inhibited the lipopolysaccharide (LPS)-induced inflammatory cytokine IL-6 and TNF-α production in RAW 264.7 cells. In addition, the luciferase reporter assay results of different subtypes of the peroxisome proliferator-activated receptor (PPAR) showed that nuciferine dose-dependently activated all the PPAR activities. Specific inhibitors of PPARα and PPARγ significantly abolished the production of inflammatory cytokines as well as IκBα degradation. However, PPARδ inhibitor did not show this effect. Our results suggested a potential molecular mechanism of the anti-inflammatory effects of nuciferine in LPS-induced inflammation, at least in part, by activating PPARα and PPARγ in RAW 264.7 cells.

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Docosahexaenoic acid attenuates LPS-stimulated inflammatory response by regulating the PPARγ/NF-κB pathways in primary bovine mammary epithelial cells

Cited by 56 publications

References 32 publications

Docosahexaenoic Acid Inhibits Expression of Fibrotic Mediators in Mice With Chronic Pancreatitis

Docosahexaenoic Acid Inhibits Expression of Fibrotic Mediators in Mice With Chronic Pancreatitis

Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Nuciferine Inhibits Proinflammatory Cytokines via the PPARs in LPS-Induced RAW264.7 Cells

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