Do senescence markers correlate in vitro and in situ within individual human donors?

Waaijer, Mariette E. C.; Gunn, David A.; Heemst, Diana van; Slagboom, P. Eline; Sedivy, John M.; Dirks, Roeland W.; Tanke, Hans J.; Westendorp, Rudi G. J.; Maier, Andrea B.

doi:10.18632/aging.101389

Cited by 15 publications

(7 citation statements)

References 37 publications

(43 reference statements)

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“…Loss of lamin B1 protein was suggested to be a promising biomarker for senescence but its mRNA levels failed to distinguish senescence vs quiescent fibroblasts [120]. Several groups came up with various senescence-associated markers such as γ-H2A-X and 53BP1 foci [121], activated ataxia-telangiectasia mutated (ATM) kinase [122], telomere-associated DNA damage foci (TAF) [116,123,124,125], and a histone variant H2A.J [126]. Most of them were either employed individually or in combination, but the identification of a unique senescent cell-specific marker remains unsolved [127].…”

Section: Discussionmentioning

confidence: 99%

LncRNAs Regulatory Networks in Cellular Senescence

Puvvula

2019

IJMS

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Long noncoding RNAs (lncRNAs) are a class of transcripts longer than 200 nucleotides with no open reading frame. They play a key role in the regulation of cellular processes such as genome integrity, chromatin organization, gene expression, translation regulation, and signal transduction. Recent studies indicated that lncRNAs are not only dysregulated in different types of diseases but also function as direct effectors or mediators for many pathological symptoms. This review focuses on the current findings of the lncRNAs and their dysregulated signaling pathways in senescence. Different functional mechanisms of lncRNAs and their downstream signaling pathways are integrated to provide a bird’s-eye view of lncRNA networks in senescence. This review not only highlights the role of lncRNAs in cell fate decision but also discusses how several feedback loops are interconnected to execute persistent senescence response. Finally, the significance of lncRNAs in senescence-associated diseases and their therapeutic and diagnostic potentials are highlighted.

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Section: Discussionmentioning

confidence: 99%

LncRNAs Regulatory Networks in Cellular Senescence

Puvvula

2019

IJMS

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“…Persistent DNA damage is a critical trigger of cellular senescence and can be identified by the presence of γ-H2A-X and 53BP1 foci ( D’Adda Di Fagagna et al, 2003 ) and activated ataxia-telangiectasia mutated (ATM) kinase ( Zou, 2007 ). However, while DNA damage per se , is not a marker for cellular senescence, the occurrence of telomere-associated DNA damage foci (TAF) has been used to detect senescent cells and quantify tissue aging ( Herbig et al, 2006 ; Jeyapalan et al, 2007 ; Hewitt et al, 2012 ; Waaijer et al, 2018 ). Replicative senescent baboon skin fibroblasts and skin biopsies from aged baboons displayed high incidence of TAF as indicated by co-localization of 53BP1 and γ-H2A-X on telomeric DNA ( Herbig et al, 2006 ; Jeyapalan et al, 2007 ).…”

Section: Senescence Biomarkersmentioning

confidence: 99%

Biomarkers of Cellular Senescence and Skin Aging

2018

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Cellular senescence is an irreversible growth arrest that occurs as a result of different damaging stimuli, including DNA damage, telomere shortening and dysfunction or oncogenic stress. Senescent cells exert a pleotropic effect on development, tissue aging and regeneration, inflammation, wound healing and tumor suppression. Strategies to remove senescent cells from aging tissues or preneoplastic lesions can delay tissue dysfunction and lead to increased healthspan. However, a significant hurdle in the aging field has been the identification of a universal biomarker that facilitates the unequivocal detection and quantification of senescent cell types in vitro and in vivo. Mammalian skin is the largest organ of the human body and consists of different cell types and compartments. Skin provides a physical barrier against harmful microbes, toxins, and protects us from ultraviolet radiation. Increasing evidence suggests that senescent cells accumulate in chronologically aged and photoaged skin; and may contribute to age-related skin changes and pathologies. Here, we highlight current biomarkers to detect senescent cells and review their utility in the context of skin aging. In particular, we discuss the efficacy of biomarkers to detect senescence within different skin compartments and cell types, and how they may contribute to myriad manifestations of skin aging and age-related skin pathologies.

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“…Persistent ROS-induced DNA damage drives cellular senescence and can be identified by markers for DNA double-strand breaks, such as γH2AX, that are co-localized with DNA damage checkpoint factors including p53-binding protein 1 (53BP1), mediator of DNA damage checkpoint protein 1, and Nijmegen breakage syndrome 1 [ 33 ]. In addition, the occurrence of telomere-associated DNA damage foci (TAF) has been used to detect senescent cells and quantify tissue aging in situ [ 34 , 35 ]. Replicative senescent baboon skin fibroblasts and skin tissues from aged baboons revealed increased TAF as indicated by co-localization of 53BP1 and γH2AX on telomeric DNA [ 16 , 36 ].…”

Section: Fibroblast Senescencementioning

confidence: 99%

Cellular Senescence and Inflammaging in the Skin Microenvironment

Lee

Choi

Roh

et al. 2021

IJMS

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Cellular senescence and aging result in a reduced ability to manage persistent types of inflammation. Thus, the chronic low-level inflammation associated with aging phenotype is called “inflammaging”. Inflammaging is not only related with age-associated chronic systemic diseases such as cardiovascular disease and diabetes, but also skin aging. As the largest organ of the body, skin is continuously exposed to external stressors such as UV radiation, air particulate matter, and human microbiome. In this review article, we present mechanisms for accumulation of senescence cells in different compartments of the skin based on cell types, and their association with skin resident immune cells to describe changes in cutaneous immunity during the aging process.

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Do senescence markers correlate in vitro and in situ within individual human donors?

Cited by 15 publications

References 37 publications

LncRNAs Regulatory Networks in Cellular Senescence

LncRNAs Regulatory Networks in Cellular Senescence

Biomarkers of Cellular Senescence and Skin Aging

Cellular Senescence and Inflammaging in the Skin Microenvironment

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