Do progenitor cells from different tissue have the same phenotype?

Godoy, Roberta Ferro de; Alves, Ana Liz Garcia; Gibson, Amanda J.; Lima, Eduardo Maurício Mendes de; Goodship, Allen E.

doi:10.1016/j.rvsc.2014.02.013

Cited by 15 publications

(13 citation statements)

References 16 publications

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“…A problem in the study and characterization of any sources of MSCs isolated from ruminants as well as other species, except human, is the lack of species specific monoclonal antibodies . Godoy et al have suggested that ovine MSCs isolated from different tissues, such as bone marrow, synovial membrane and adipose tissues, are positive for CD44 and MHC class I . Applying flow‐cytometry analysis for the MSC surface markers in our present study, we have shown that the isolated ovine fetal BM‐MSCs were positive for CD166 and CD44; however, the expression of CD90 was weak.…”

Section: Discussionsupporting

confidence: 61%

“…24 Godoy et al have suggested that ovine MSCs isolated from different tissues, such as bone marrow, synovial membrane and adipose tissues, are positive for CD44 and MHC class I. 25 Applying flow-cytometry analysis for the MSC surface markers in our present study, we have shown that the isolated ovine fetal BM-MSCs were positive for CD166 and CD44; however, the expression of CD90 was weak. While different laboratories around the world have focused their studies on pre-clinical experiments through the application of MSCs that have taken their origin from non-human beings, however, as yet there is not a specific panel of such CD markers available for identification of MSCs for such non-human source of MSCs.…”

Section: Discussionsupporting

confidence: 57%

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Ovine fetal mesenchymal stem cell differentiation to cardiomyocytes, effects of co‐culture, role of small molecules; reversine and 5‐azacytidine

Soltani

Rahmani

Joupari

et al. 2016

Cell Biochemistry & Function

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The aim of the present study was to investigate the effect of small molecules: Reversine and 5-azacytidine (5-AC), in an indirect co-culture condition with the cardiac fibroblasts as well as non co-culture condition, in order to explore the effect of such molecules in the process of differentiation of the ovine bone-marrow mesenchymal stem cells (BM-MSCs) towards cardiomyocytes. Surface antigens of the isolated cells were analysed using flow-cytometry. In addition, following to three passages cells were examined for their differentiation capacity into osteocytes and adipose cells, in order to ensure the mesenchymal origin of the stem cells. Six types of treatments were carried out in the present investigation, such that, in the first treatment BM-MSCs were cultured for 28 days as control group; the second treatment was composed of culturing ovine fetal cardiac fibroblasts on inserts, aiming to use these inserts for culturing plates which were seeded with BM-MSCs (Chamber group). As the third treatment, BM-MSCs were supplemented with 10-μM 5-AC and incubated for 48 h. The fourth treatment was composed of supplementing BM-MSCs with the 600-nM reversine, incubated for 48 h, and subsequently the incubation was further extended for another 48 h in the presence of 5-AC. The fifth treatment was composed of supplementing the chamber group with 10-μM 5-AC and incubation for 48 h, and the last or the sixth treatment was such that chamber group was supplemented with 600-nM reversine and an incubation period of 48 h. Following to the incubation, medium was replaced with 10-μM 5-AC and further incubated for another round of 48 h. In all treatments, following to addition of the small molecules incubations were carried out for 28 days; same as controls. Expression of cardiac alpha-actinin was analysed by immunocytochemistry. BM-MSCs have shown to express CD44 and CD166 along with a weak expression of the CD90, CD34, in addition to CD45. Multilineage differentiation has indicated that BM-MSCs could differentiate into adipose and osteocytes cells as well. In the treatment 4 it was observed that FGF signalling involved genes and all cardiac-related genes (ANP, MYH6 and Troponin I) were significantly expressed, except connexin 43 compared to other treatments. All treatments received small molecules, either alone or as a co-culture were seen to express sarcomeric alpha-actinin. This finding was partially supported by immunocytochemistry. These results validate that reversine and 5-AC have an effect on ovine BM-MSC differentiation into cardiomyocytes. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionsupporting

confidence: 57%

Ovine fetal mesenchymal stem cell differentiation to cardiomyocytes, effects of co‐culture, role of small molecules; reversine and 5‐azacytidine

Soltani

Rahmani

Joupari

et al. 2016

Cell Biochemistry & Function

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“…Godoy et al (2014) used sheep to compare stem cells from bone marrow, adipose tissue and synovium [17]. Their research indicated that MSCs could be harvested from each of these sources and that MSCs from each source shared the ability to from bone, cartilage and fat as well as sharing a similar immunophenotype based on expression of the marker CD44.…”

Section: Introductionmentioning

confidence: 99%

Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair

Hindle

Baily

Khan

et al. 2016

Stem Cells and Development

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Publisher Rights Statement:This is the final peer-reviewed manuscript as accepted for publication General rightsCopyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policyThe University of Edinburgh has made every reasonable effort to ensure that Edinburgh Research Explorer content complies with UK legislation. If you believe that the public display of this file breaches copyright please contact openaccess@ed.ac.uk providing details, and we will remove access to the work immediately and investigate your claim. Immunohistochemistry and fluorescence-activated cell sorting (FACS) were used to ascertain the reactivity of anti-human and anti-ovine antibodies, which were combined and used to identify and isolate pericytes (CD34-CD45-CD146+) and adventitial cells (CD34+CD45-CD146-). The purified cells demonstrated osteogenic, adipogenic and chondrogenic potential in culture.Autologous ovine PSCs (oPSCs) were isolated, cultured and efficiently transfected using a GFP encoding lentivirus. The cells were implanted into articular cartilage defects on the medial femoral condyle using a hydrogel and collagen membranes.Four weeks following implantation the condyle was explanted and confocal laser scanning microscopy demonstrated the presence of oPSCs in the defect repaired with the hydrogel.These data suggest the testability in a large animal of native mesenchymal stem cell autologous grafting, thus avoiding possible biases associated with xenotransplantation.Such a setting will be used in priority for indications in orthopaedics, at first to model articular cartilage repair.

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“…According to the International Society of Cellular Therapy (ISCT), MSCs are characterized based on three important criteria: cell adherence, when cultured in standard conditions [38,39], expression of specific cell surface markers (cluster of differentiation (CD)73, CD90, and CD105) and negative to others (CD45, CD34, CD11b, CD79a, and HLA-DR), and differentiation potential in vitro into multiple lineages: osteogenic, adipogenic, and chondrogenic, in defined culture conditions [38,39]. They are furthermore characterized with respect to colony-forming unit fibroblast (CFU-F) [38,40].…”

Section: Msc Sources Applicable For Musculoskeletal Regenerationmentioning

confidence: 99%

“…The parallel expression or exclusion of several cell surface markers, associated with other ISCT criteria, is a frequent approach to MSC identification [41]. They share nonhematopoietic cell surface markers, such as CD29, CD44, CD73, CD90, and CD105 and human lymphocyte antigen (HLA) [29]; CD9, CD44, CD54, CD90, CD166 [41]; and CD44, CD79, CD90, CD105 [39]. They are usually negative for hematopoietic markers, such as CD34 e CD45 [39].…”

Section: Msc Sources Applicable For Musculoskeletal Regenerationmentioning

confidence: 99%

Synovia-Derived Mesenchymal Stem Cell Application in Musculoskeletal Injuries: A Review

Branquinho¹,

Caseiro²,

SantosPedrosa³

et al. 2018

Tissue Regeneration

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Musculoskeletal injuries impact millions of people globally and affect their health and well-being as well as of their companion and athletic animals. Soft-tissue injuries represent almost half of these and are associated with unorganized scar tissue formation and long time-depending healing processes. Cell-based therapeutic strategies have been developed in the past decades aiming at the treatment and reversion of such disorders. Stem cells are fairly appealing in the field, being a responsive undifferentiated population, with ability to self-renew and differentiate into different lineages. Mesenchymal stem cells (MSCs) can be obtained from several adult tissues, including the synovial membrane. Synovia-derived MSCs can be found in individuals of any age and are associated to intrinsic regenerative processes, through both paracrine and cell-to-cell interactions, thus, contributing to hosts' healing capacity. Studies have demonstrated the potential benefit of synovia-derived MSCs in these regenerative processes in both human and veterinary medicine. The purpose of this chapter is to review the literature regarding SM-MSC therapies applied to musculoskeletal disorders, in both human and veterinary medicine.

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Do progenitor cells from different tissue have the same phenotype?

Cited by 15 publications

References 16 publications

Ovine fetal mesenchymal stem cell differentiation to cardiomyocytes, effects of co‐culture, role of small molecules; reversine and 5‐azacytidine

Ovine fetal mesenchymal stem cell differentiation to cardiomyocytes, effects of co‐culture, role of small molecules; reversine and 5‐azacytidine

Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair

Synovia-Derived Mesenchymal Stem Cell Application in Musculoskeletal Injuries: A Review

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