Molecularly imprinted polymer nanoparticles (MIP NPs) are antibody-like recognitionm aterials prepared by at emplate-assisted synthesis. MIP NPs able to target biomolecules, like proteins, are under the spotlightf or their great potential in medicine, but efficiently imprinting biological templates is still very challenging. Here we propose generating am olecular imprinti ns ingle NPs, by photochemically initiating the polymerizationf rom individual protein templates.I nt his way,e ach protein molecule tailors itself its own "polymeric dress". For this, the template protein is covalently coupledw ith ap hotoinitiator,E osin Y. Irradiatedw ith light at 533 nm, the Eosin moiety acts as an antenna and transfers energyt oac o-initiator (an amine), which generates ar adical and initiates polymerization. As ar esult, ap olymer network is forming only around the very template molecule, producing crosslinked NPs of 50 nm, with singleb inding sites showing high affinity (K D 10 À9 m)f or their biologicalt arget, and selectivityo ver other proteins.