Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research?

Abstract

The aim of this study was to evaluate the antimicrobial action of different endodontic pastes against Enterococcus faecalis ATCC 29212, isolated from the urinary tract, and compare the action with E. faecalis ATCC 4083, isolated from the root canal. For this purpose, dentin blocks were infected for 21 days with both bacteria at different time-intervals to ensure there would be no cross contamination. After this period, blocks were immersed in the test medications for 7 days, according to the following groups: CH/S, CH/P, CH/CMCP, CH/CHX, CH/DAP and TAP. Images of the samples were captured with a confocal microscope and the percentage of live cells was computed by means of the Bioimage program. The ATCC 29212 strain was shown to be more resistant to CH/SS, Calen, CH/DAP, and TAP than the ATCC 4083 strain. The antimicrobial action of the medications against each strain were divergent concerning the order of susceptibility. The authors concluded that the strains behaved in a different manner: in general, those extracted from the urinary tract were more resistant to the tested medications. Therefore, when E. faecalis must be used for in vitro research in endodontics, we suggest the use of ATCC 4083 strain to obtain results that are closer to the clinical reality.

Effect of Intracanal Medicaments on Pull-Out Bond Strength of Fiber Post Cemented with a Self-Adhesive System

“…11 12 It has been shown that both medicaments appear to have promise as alternative for CH therapy in cases in which symptoms cannot be easily subsided. 13 14…”

Análise do pH e da atividade antimicrobiana de um novo medicamento intracanal biocerâmico Bio-C Temp

“…Apesar do conhecimento do perfil multiespécie da infecção endodôntica, as avaliações dos biofilmes monoespécies de E. faecalis são fundamentais, pois esses microrganismos são mais resistentes à restrição de nutrientes na coexistência com outras espécies presentes nas infecções endodônticas (Gao, et al, 2016), mostrando maior sobrevida. A cepa de E. faecalis mais utilizada nos estudos in vitro é a ATCC 29212, com origem no trato geniturinário (Kangarlou, et al, 2016;Collares, et al, 2017;Cheng, et al, 2017;Swimberghe, et al, 2018), no entanto, o uso de cepas isoladas do canal radicular é recomendado, já que diferentes cepas da mesma espécie podem diferir devido a características adquiridas (Wang, et al, 2014;Barros, et al, 2014;Zancan, et al, 2018). Diante disso, optou-se pela inclusão de uma cepa isolada de canais radiculares necrosados (ATCC 4083) (Zancan, et al, 2018;Oda, et al, 2019).…”

“…A cepa de E. faecalis mais utilizada nos estudos in vitro é a ATCC 29212, com origem no trato geniturinário (Kangarlou, et al, 2016;Collares, et al, 2017;Cheng, et al, 2017;Swimberghe, et al, 2018), no entanto, o uso de cepas isoladas do canal radicular é recomendado, já que diferentes cepas da mesma espécie podem diferir devido a características adquiridas (Wang, et al, 2014;Barros, et al, 2014;Zancan, et al, 2018). Diante disso, optou-se pela inclusão de uma cepa isolada de canais radiculares necrosados (ATCC 4083) (Zancan, et al, 2018;Oda, et al, 2019). Nesse estudo, não houve diferença significante com os resultados encontrados comparando-se as cepas.…”

Antibacterial Activity of Phytochemical Extracts and Endophytic Fungi of Carapa Guianensis Against Enterococcus Faecalis in Endodontic Infections An In Vitro Study

“…The viability of the remaining microorganisms after instrumentation and disinfection of root canals contributes significantly to endodontic treatment failure [1]. In the absence of clinical evidence that intracanal medicaments are sufficiently effective against persistent bacterial infections, there is a need for further investigation of new medicaments for use in periradicular conditions [2].…”

“…The success of endodontic treatment is related to the elimination of microorganisms from the root canal system [1,2]. Persistent infection after endodontic treatment, resulting from remnants of bacteria in root canal systems in areas of anatomical complexity and difficult access, continues to present a challenge in endodontics [1]. Thus, recurrent periapical infections are mainly associated with Enterococcus faecalis [2].…”