dNK derived IFN-γ mediates VSMC migration and apoptosis via the induction of LncRNA MEG3: A role in uterovascular transformation

Liu, Weifang; Liu, Xiaoxia; Luo, Moulun; Li, Xiaoping; Luo, Qingqing; Tao, Hui; Wu, Di; Lu, Sisi; Jin, Jing; Zhao, Yin; Zou, Li

doi:10.1016/j.placenta.2016.12.023

Cited by 41 publications

(34 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To exclude the interference of proliferation, a CCK-8 assay was conducted and the result showed that MEG3 had no significant effect on trophoblast proliferation (Supporting Information Figure S2). In addition, mounting evidence proved that MEG3 could regulate the production of MMP2 (Liu et al, 2017;Piccoli et al, 2017a;Piccoli et al, 2017b). Therefore, we detected the expression were consistent with the findings of many cancer studies, including breast cancer, thyroid carcinoma, and gastric cancer; they thought MEG3 could slow cancer progression by inhibiting the migration and invasion of tumor cells (Peng et al, 2015;Sun et al, 2016;Wang et al, 2015).…”

Section: Discussionsupporting

confidence: 87%

“…To exclude the interference of proliferation, a CCK‐8 assay was conducted and the result showed that MEG3 had no significant effect on trophoblast proliferation (Supporting Information Figure S2). In addition, mounting evidence proved that MEG3 could regulate the production of MMP2 (Liu et al, ; Piccoli et al, ; Piccoli et al, 2017b). Therefore, we detected the expression of MMP2 from transcriptional and translational levels and demonstrated its reduction as MEG3 upregulated in HTR‐8/SVneo cells.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

LncRNA MEG3 inhibits trophoblast invasion and trophoblast‐mediated VSMC loss in uterine spiral artery remodeling

Tao

Liu

et al. 2019

Molecular Reproduction Devel

Self Cite

View full text Add to dashboard Cite

Extravillous trophoblasts (EVTs) migrate into uterine decidua and induce vascular smooth muscle cell (VSMC) loss through mechanisms thought to involve migration and apoptosis, achieving complete spiral artery remodeling. Long noncoding RNA maternally expressed gene 3 (MEG3) can regulate diverse cellular processes, such as proliferation and migration, and has been discovered highly expressed in human placenta tissues. However, little is known about the role of MEG3 in modulating EVT functions and EVT‐induced VSMC loss. In this study, we first examined the location of MEG3 in human first‐trimester placenta by in situ hybridization. Then, exogenous upregulation of MEG3 in HTR‐8/SVneo cells was performed to investigate the effects of MEG3 on EVT motility and EVT capacity to displace VSMCs. Meanwhile, the molecules mediating EVT‐induced VSMC loss, such as tumor necrosis factor‐α (TNF‐α), Fas ligand (FasL), and tumor necrosis factor‐α‐related apoptosis‐inducing ligand (TRAIL) were detected at transcriptional and translational levels. Finally, VSMCs were cocultured with MEG3‐upregulated HTR‐8/SVneo to explore the role of MEG3 on EVT‐mediated VSMC migration and apoptosis. Results showed that MEG3 was expressed in trophoblasts in placental villi and decidua, and MEG3 enhancement inhibited HTR‐8/SVneo migration and invasion. Meanwhile, the displacement of VSMCs by HTR‐8/SVneo and the expression of TNF‐α, FasL and TRAIL in HTR‐8/SVneo were reduced following MEG3 overexpression in HTR‐8/SVneo. Furthermore, HTR‐8/SVneo with MEG3 upregulation impaired VSMC migration and apoptosis. The PI3K/Akt pathway, which is possibly downstream, was inactivated in MEG3‐upregulated HTR‐8/SVneo. These findings suggest that MEG3 might be a negative regulator of spiral artery remodeling via suppressing EVT invasion and EVT‐mediated VSMC loss.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

LncRNA MEG3 inhibits trophoblast invasion and trophoblast‐mediated VSMC loss in uterine spiral artery remodeling

Tao

Liu

et al. 2019

Molecular Reproduction Devel

Self Cite

View full text Add to dashboard Cite

show abstract

“…A study reports that long intergenic noncoding RNA 00305 sponges miR‐136 to regulate the hypoxia‐induced apoptosis of human umbilical vein endothelial cells . And 1 study exhibits that lncRNA MEG3 is regulated by dNK‐derived interferon gamma (IFN‐γ), stimulates cells migration, inhibits cells proliferation, and promotes cell apoptosis in human aorta VSMC (HA‐VSMC) cell line . Besides, lncRNA TUG1 sponges miR‐204‐5p to promote osteoblast differentiation through upregulating Runx2 in aortic valve calcification .…”

Section: Discussionmentioning

confidence: 99%

Circulating lncRNA IFNG‐AS1 expression correlates with increased disease risk, higher disease severity and elevated inflammation in patients with coronary artery disease

Shao

2018

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

“…dNK cells are the main immune cells in the maternal‐fetal interface, and are involved in tissue building and remodeling via secretion of angiogenic factors . Vascular smooth muscle cell (VSMC) loss and separation, involving cell migration and apoptosis, plays an important role in appropriate spiral artery remodeling, which is important for fetal development and pregnancy outcomes . Co‐culture of dNK cells and VSMC can positively regulate expression of the lncRNA MEG3 in VSMC, inhibiting VSMC proliferation, promoting VSMC migration, and inducing VSMC apoptosis .…”

Section: Gene Regulation By Lncrna In Nk Cellsmentioning

confidence: 99%

“…Vascular smooth muscle cell (VSMC) loss and separation, involving cell migration and apoptosis, plays an important role in appropriate spiral artery remodeling, which is important for fetal development and pregnancy outcomes . Co‐culture of dNK cells and VSMC can positively regulate expression of the lncRNA MEG3 in VSMC, inhibiting VSMC proliferation, promoting VSMC migration, and inducing VSMC apoptosis . Thus, dNK cells facilitate the creation of the appropriate microenvironment for placental and fetal development, which is important for a successful pregnancy.…”

Section: Gene Regulation By Lncrna In Nk Cellsmentioning

confidence: 99%

Noncoding RNA in NK cells

Yang

Shen

Feng

et al. 2018

Journal of Leukocyte Biology

View full text Add to dashboard Cite

Noncoding RNAs (ncRNA) are important regulators that modulate cell proliferation, apoptosis, the cell cycle, and DNA methylation. NK cells mediate the immune response via the secretion of various cytokines and are important innate immune cells in the human immune system. Recent studies have found that ncRNA plays an important role in NK cell development and function. With recent advances in bioinformatics and next-generation sequencing, novel ncRNAs have been identified, allowing us to more fully appreciate its functions in NK cell biology. In this review, we summarize and discuss the latest studies on the functions and regulatory mechanisms of long noncoding RNA (lncRNA) and microRNA in NK cells from the viewpoint of epigenetic mechanisms to help us clearly understand ncRNA in NK cells. K E Y W O R D Scytokines, gene regulation mechanism, immune response, lncRNA, microRNA, NK cell the roles of ncRNA, mainly lncRNA and microRNA, in NK cell development and function. We also summarize the latest studies and discuss the potential regulation of NK cell biologic processes by lncRNA and microRNA.

show abstract

dNK derived IFN-γ mediates VSMC migration and apoptosis via the induction of LncRNA MEG3: A role in uterovascular transformation

Cited by 41 publications

References 42 publications

LncRNA MEG3 inhibits trophoblast invasion and trophoblast‐mediated VSMC loss in uterine spiral artery remodeling

LncRNA MEG3 inhibits trophoblast invasion and trophoblast‐mediated VSMC loss in uterine spiral artery remodeling

Circulating lncRNA IFNG‐AS1 expression correlates with increased disease risk, higher disease severity and elevated inflammation in patients with coronary artery disease

Noncoding RNA in NK cells

Contact Info

Product

Resources

About