Background: DNA2 helicase/nuclease participates in telomere maintenance by unknown mechanisms. Results: dna2 mutants show slightly shortened telomeric GT-overhangs and accumulate small, nascent lagging strand DNA chains at telomeres. Conclusion: Unlike at DSBs, additional nucleases largely compensate for the simultaneous loss of Dna2 and Mre11 in producing GT. Significance: FEN1, Dna2, or Exo1 is necessary to mature lagging strands at telomeres.
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