DNA templated self-assembly of gold nanoparticle clusters in the colorimetric detection of plant viral DNA using a gold nanoparticle conjugated bifunctional oligonucleotide probe

Gunasekaran, Dharanivasan; Riyaz, S.U. Mohammed; Jesse, Denison Michael Immanuel; Muthuramalingam, T. Raja; Rajendran, Ganapathy; Kathiravan, K.

doi:10.1039/c5ra25559g

Cited by 36 publications

(26 citation statements)

References 69 publications

(71 reference statements)

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“…Another possible explanation, which we explored, was that one of the reagents used for preparing the protein for SDS-PAGE separation may have possibly interfered with the protein-gold interactions, effectively freeing the involved proteins from the nanoparticles. The primary candidate considered for this interference was β-mercaptoethanol (βME), as it is known to function as a highly efficient capping/quenching agent for gold nanoparticle syntheses capable of breaking Au-S bonds between nanoparticles and protein 54 . To determine whether βME was responsible for this freeing of Au-bound proteins, we were prompted to run an additional gel comparing protein migration from the fractions of cells either treated with Au-PEG, Na-PEG, or no treatment in duplicate-half would receive βME and the other half would not ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold

et al. 2020

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Various cancer cells have been demonstrated to have the capacity to form plasmonic gold nanoparticles when chloroauric acid is introduced to their cellular microenvironment. But their biomedical applications are limited, particularly considering the millimolar concentrations and longer incubation period of ionic gold. Here, we describe a simplistic method of intracellular biomineralization to produce plasmonic gold nanoparticles at micromolar concentrations within 30 min of application utilizing polyethylene glycol as delivery vector for ionic gold. We have characterized this process for intracellular gold nanoparticle formation, which progressively accumulates proteins as the ionic gold clusters migrate to the nucleus. This nano-vectorized application of ionic gold emphasizes its potential biomedical opportunities while reducing the quantity of ionic gold and required incubation time. To demonstrate its biomedical potential, we further induce in-situ biosynthesis of gold nanoparticles within MCF7 tumor mouse xenografts which is followed by its photothermal remediation.

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Section: Resultsmentioning

confidence: 99%

Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold

et al. 2020

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“…These methods have also been shown to have better sensitivity and shorter turnover time than conventional molecular methods such as PCR and ELISA. Many human pathogens such as Mycobacterium tuberculosis, Leishmania spp., and E. coli and plant pathogens such as tomato leaf curl virus have been developed with functionalized AuNPs (Baptista et al 2006;Soo et al 2009;Padmavathy et al 2012;Andreadou et al 2014;Larguinho et al 2015;Dharanivasan et al 2016). The paper-based gene sensor was developed for the detection of BBTV using a probe-conjugated AuNP (Wei et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Cloning of BBTV (Banana Bunchy Top Virus) components and screening of BBTV using functionalized gold nanoparticles

2017

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Banana bunchy top virus (BBTV) affects all varieties of banana plants and causes heavy economic loss in most of the banana cultivating areas. The BBTV genome comprises of six DNA components; in this study, we have cloned the six BBTV-DNA components from one of the BBTV-infected plants (Tri-8) and were submitted to GenBank. Analysis of the BBTV DNA-R component showed that it belonged to south Pacific group. Resistance against BBTV has not been observed so far in banana plants and removal and killing of the infected plants has been routinely practiced. Hence, early detection of BBTV infection would be desirable and various detection methods routinely employed include enzyme linked immunosorbent assay (antigen-antibody based) and molecular-based methods such as polymerase chain reaction (PCR), qPCR, or LAMP PCR. Most of these methods require enzymes or antibodies for detection and hence are expensive. Here, we report a visual detection method (AuNP probe assay) using gold nanoparticles (AuNPs) functionalized with an ssDNA-thiolated probe (CR1). This method is based on the hybridization of the functionalized AuNPs with the target DNA (BBTV). In the AuNP probe assay, the functionalized AuNPs retains red colour when BBTV DNA is present, and in the absence of BBTV DNA, the colour of the functionalized AuNPs changes to purple when salt is added. The AuNP probe assay was compared with PCR for the detection of banana plants and it was found that AuNP probe assay was better than PCR in detecting BBTV infection (86.5% for AuNP probe assay and 65% for PCR). The AuNP probe assay was found to be highly specific to BBTV and was found to detect up to 1 pg/μl of the plasmid (pTZBBTri 4, BBTV DNA) mixed with healthy banana DNA.

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“…Au nanoparticle-based (AuNP) colorimetric assays have been used for fast, cost-effective, and accurate analysis in environmental and biological samples [21][22][23][24][25][26]. In those approaches, AuNPs usually functionalized by specific ligands are monodispersed because of electrostatic exclusive force or steric hindrance.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Visual and photometric determination of histamine using unmodified gold nanoparticles

et al. 2017

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The authors describe a colorimetric approach for on-site monitoring of histamine based on the use of gold nanoparticles (AuNPs) that have a negatively charged surface due to the presence of adsorbed citrate ions. Histamine has two basic functional groups, an aliphatic amino group and an imidazole ring. Under the experimental conditions, the protonated aliphatic amino group drives the imidazole ring into close proximity to the AuNPs due to electrostatic attraction. This accelerates the replacement of citrate ions by the imidazole ring because of the strong affinity between imidazole and AuNPs. As a consequence, the two groups synergistically induce the aggregation of the AuNPs and trigger a visible color change from red to blue. The minimum visually detectable histamine concentration is 1.81 μM, which is comparable to the limit of detection (LOD) of the electrochemical approaches at a signal-to-noise ratio of 3:1. When using absorbance at 522 nm, the LOD is lowered to 38 nM. The method was applied to the determination of histamine in fish samples.

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DNA templated self-assembly of gold nanoparticle clusters in the colorimetric detection of plant viral DNA using a gold nanoparticle conjugated bifunctional oligonucleotide probe

Abstract: The DNA templated self-assembly of gold nanoparticles clustered in different configurations (nn = 2–∞) was investigated in the colorimetric detection of ToLCNDV DNA using a gold nanoparticle conjugated bifunctional oligonucleotide probe.

Cited by 36 publications

References 69 publications

Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold

Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold

Cloning of BBTV (Banana Bunchy Top Virus) components and screening of BBTV using functionalized gold nanoparticles

Visual and photometric determination of histamine using unmodified gold nanoparticles

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