DNA targeting by compact Cas9d and its resurrected ancestor
Rodrigo Fregoso Ocampo,
Jack P. K. Bravo,
Tyler Dangerfield
et al.
Abstract:The type II-A CRISPR effector SpCas9 has gained widespread popularity as an efficient and programmable genome editing tool. However, much remains to be known about novel compact variants that may overcome some limitations of current systems (1,2). Recently, alternative CRISPR-Cas systems with highly compact nucleases capable of genome editing in mammalian cells have been discovered through metagenomic analysis of uncultivated microbes, including Cas9d (a type II-D CRISPR-Cas effector) (3). Here, we report the … Show more
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