DNA repair is responsible for the presence of oxidatively damaged DNA lesions in urine

Cooke, Marcus S.; Evans, Mark D.; Dove, Rosamund; Różalski, Rafał; Gackowski, Daniel; Siomek, Agnieszka; Lunec, Joseph; Oliński, Ryszard

doi:10.1016/j.mrfmmm.2005.01.022

Cited by 175 publications

(113 citation statements)

References 41 publications

Supporting

Mentioning

111

Contrasting

Unclassified

Order By: Relevance

“…We found a significant trend for f30% lower breast cancer risk with increased 8-oxodG excretion. Although the nucleotide pool may be a significant source (7), recent data suggest that 8-oxodG in urine is the result of DNA repair and does not result from diet or cell death (27). Thus, our data indicating lower risk at higher levels of urinary 8-oxodG may be related to higher DNA repair activity.…”

Section: Discussionmentioning

confidence: 55%

Relationship between Urinary 15-F2t-Isoprostane and 8-Oxodeoxyguanosine Levels and Breast Cancer Risk

Rössner

Gammon

Terry

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

View full text Add to dashboard Cite

To evaluate the role of oxidative stress in breast cancer, we measured urinary levels of 15-F 2t -isoprostane (15-F 2t -IsoP) and 8-oxodeoxyguanosine (8-oxodG) in 400 cases and 401 controls, participants of the Long Island Breast Cancer Study Project. We also analyzed the effect of different factors that are associated with oxidative stress and might influence 15-F 2t -IsoP and 8-oxodG levels. We observed a statistically significant trend in breast cancer risk with increasing quartiles of 15-F 2t -IsoP levels [odds ratio (OR), 1.25; 95% confidence interval (95% CI), 0.81-1.94; OR, 1.53; 95% CI, 0.99-2.35; OR, 1.88; 95% CI, 1.23-2.88, for the 2nd, 3rd, and 4th quartile relative to the lowest quartile, respectively; P trend = 0.002]. Although it is possible that increased levels may reflect the stress associated with recent treatment, the positive association was also observed when the analyses were restricted to case women for whom chemotherapy and radiation therapy had not yet been initiated at the time of the urine collection. The association with the highest quartile compared with lowest quartile of 15-F 2t -IsoP was similar across strata of age, physical activity, fruit and vegetable intake, alcohol intake, cigarette smoking, body mass index, and menopausal status. We did not observe any association of breast cancer risk with 8-oxodG levels, but when cases with radiation treatment were removed from the analysis, a significant inverse trend (P = 0.04) was observed. Among controls, levels of 15-F 2t -IsoP were higher among current cigarette smokers but did not differ by the amount of physical activity, fruit and vegetable intake, alcohol intake, body mass index, and menopausal status. Among controls, levels of 8-oxodG were higher among postmenopausal women and current and former cigarette smokers but did not differ by the other factors. In summary, our results suggest that urinary markers of lipid peroxidation and oxidative DNA damage may be associated with breast cancer risk. (Cancer Epidemiol Biomarkers Prev 2006;15(4):639 -44)

show abstract

Section: Discussionmentioning

confidence: 55%

Relationship between Urinary 15-F2t-Isoprostane and 8-Oxodeoxyguanosine Levels and Breast Cancer Risk

Rössner

Gammon

Terry

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

View full text Add to dashboard Cite

show abstract

“…8-OxodG can induce GC-TA transversion mutations particularly during DNA replication and thus, if these oxidative lesions are not repaired, they can become mutagenic (Peoples and Karnes, 2005). There is a rather broad consensus that extracellular 8-oxodG levels are not affected by diet, cell death or artefactual formation (Cooke et al, 2005). However, this is the first study to show that oxidative stress observed in cancer cells reflects a good correlation to the serum 8-oxodG levels.…”

Section: Discussionmentioning

confidence: 82%

8-Hydroxydeoxyguanosine: a new potential independent prognostic factor in breast cancer

et al. 2010

View full text Add to dashboard Cite

BACKGROUND: is the commonly used marker of oxidative stress-derived DNA damage. 8-OxodG formation is regulated by local antioxidant capacity and DNA repair enzyme activity. Earlier studies have reported contradictory data on the function of 8-oxodG as a prognostic factor in different cancer types. METHODS: We assessed pre-operative serum 8-oxodG levels with an enzyme-linked immunosorbent assay in a well-defined series of 173 breast cancer patients. 8-OxodG expression in the nuclei of cancer cells from 150 of these patients was examined by immunohistochemistry. RESULTS: The serum 8-oxodG levels and immunohistochemical 8-oxodG expression were in concordance with each other (Po0.05). Negative 8-oxodG immunostaining was an independent prognostic factor for poor breast cancer-specific survival according to the multivariate analysis (Po0.01). This observation was even more remarkable when ductal carcinomas only (n ¼ 140) were considered (Po0.001). A low serum 8-oxodG level was associated statistically significantly with lymphatic vessel invasion and a positive lymph node status. CONCLUSIONS: Low serum 8-oxodG levels and a low immunohistochemical 8-oxodG expression were associated with an aggressive breast cancer phenotype. In addition, negative 8-oxodG immunostaining was a powerful prognostic factor for breast cancer-specific death in breast carcinoma patients.

show abstract

“…6), high performance liquid chromatography with electrochemical detection (HPLC ECD; ref. 7), HPLC with single (8) or tandem (9) mass spectrometry, 32 P postlabeling (10), immunoassay (11,12), alkaline elution (13), and Comet assay (14), plus other methods based upon the nicking of DNA at oxidized bases by means of repair enzymes (15,16). However, after the publication of the findings from the European Standards Committee on Oxidative DNA Damage (17), a number of these techniques have fallen from favor (reviewed in ref.…”

Section: Introductionmentioning

confidence: 99%

Measurement and Meaning of Oxidatively Modified DNA Lesions in Urine

Cooke

Oliński²,

Loft³

2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

Self Cite

205

147

View full text Add to dashboard Cite

Background: Oxidatively generated damage to DNA has been implicated in the pathogenesis of a wide variety of diseases. The noninvasive assessment of such damage, i.e., in urine, and application to large-scale human studies are vital to understanding this role and devising intervention strategies. Methods: We have reviewed the literature to establish the status quo with regard to the methods and meaning of measuring DNA oxidation products in urine. Results: Most of the literature focus upon 8-oxo-7,8-dihydro-2 ¶-deoxyguanosine (8-oxodG), and whereas a large number of these reports concern clinical conditions, there remains (a) lack of consensus between methods, (b) possible contribution from diet and/or cell death, (c) no definitive DNA repair source of urinary 2 ¶-deoxyribonucleoside lesions, and (d) no reference ranges for healthy or diseased individuals. Conclusions: The origin of 8-oxodG is not identified; however, recent cell culture studies suggest that the

show abstract

DNA repair is responsible for the presence of oxidatively damaged DNA lesions in urine

Cited by 175 publications

References 41 publications

Relationship between Urinary 15-F2t-Isoprostane and 8-Oxodeoxyguanosine Levels and Breast Cancer Risk

Relationship between Urinary 15-F2t-Isoprostane and 8-Oxodeoxyguanosine Levels and Breast Cancer Risk

8-Hydroxydeoxyguanosine: a new potential independent prognostic factor in breast cancer

Measurement and Meaning of Oxidatively Modified DNA Lesions in Urine

Contact Info

Product

Resources

About