DNA repair enhancement of aqueous extracts of in a human volunteer study

Sheng, Yuchen; Li, L; Holmgren, Kristin; Pero, Ronald W.

doi:10.1078/0944-7113-00045

Cited by 60 publications

(59 citation statements)

References 23 publications

(30 reference statements)

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“…(Rubiaceae). To date, chemical [3][4][5] and biological studies 6,7) have been conducted by many researchers. Our recent pharmacological studies on oxindole alkaloids, which are the constituents of this herbal medicine, suggested that pteropodine and isopteropodine, which are Heteroyohimbine-type oxindole alkaloids, act as positive modulators of muscarinic M 1 and 5-HT 2 receptors, 8) and rhynchophylline and isorhynchophylline, which are Corynanthé-type oxindole alkaloids, act as noncompetitive antagonists of the N-methyl-D-aspartate (NMDA) receptor in Xenopus oocytes.…”

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confidence: 99%

New Oleanan-Type Triterpene and Cincholic Acid Glycosides from Peruvian "Una de Gato" (Uncaria tomentosa)

Kitajima

Hashimoto

Sandoval

et al. 2004

CHEMICAL & PHARMACEUTICAL BULLETIN

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The Peruvian traditional herbal medicine, "Uña de Gato" (Cat's claw), 1,2) is used for treating various ailments, including arthritis, inflammation and cancer. The plants of origin of "Uña de Gato" are Uncaria tomentosa (WILLD.) D.C. and U. guianensis (AUBL.) GMEL. (Rubiaceae). To date, chemical [3][4][5] and biological studies 6,7) have been conducted by many researchers. Our recent pharmacological studies on oxindole alkaloids, which are the constituents of this herbal medicine, suggested that pteropodine and isopteropodine, which are Heteroyohimbine-type oxindole alkaloids, act as positive modulators of muscarinic M 1 and 5-HT 2 receptors, 8) and rhynchophylline and isorhynchophylline, which are Corynanthé-type oxindole alkaloids, act as noncompetitive antagonists of the N-methyl-D-aspartate (NMDA) receptor in Xenopus oocytes. 9) We have reported the isolation of new triterpenoids 10,11) and alkaloids 12,13) from Peruvian "Uña de Gato" (plant of origin: Uncaria tomentosa). As a continuation of our chemical study, we isolated one new oleanan-type triterpene (1) and three new cincholic acid glycosides (2-4) from Peruvian "Uña de Gato", which are described herein.From the MeOH extract of "Uña de Gato" (plant of origin: Uncaria tomentosa), four new triterpenes (1-4) were isolated together with five known quinovic acid glycosides. The structures of the known compounds were deduced from spectroscopic data and confirmed by comparison with reported data.The high resolution (HR)-FAB-MS spectrum of 1 measured in the positive ion mode gave a quasi-molecular ion peak at m/z 503.3343 ([MϩH] (d 28.7, 25.7, 25.0, 18.1, 17.3, 12.8), were observed. Therefore, 1 was deduced to be an oleanan-type triterpenoid possessing two secondary hydroxyl groups, one hydroxymethyl group and one carboxyl group. The HMBC cross-peaks between the oxymethine proton at d 3.54 and the methyl carbon at d 12.8 and between both the methine proton at d 2.61 and the methyl protons at d 1.06 and the oxymethine carbon at d 72.4 indicated the existence of a hydroxyl group at C-3, as common triterpenes. Furthermore, a signal assignable to a ketone carbon was observed at d 215.8 in the 13 C-NMR spectrum. HMBC correlations were observed between the ketone carbon (d 215.8) and a singlet proton assignable to H-5 (d 2.61), and between the same carbon and methylene protons assignable to H 2 -7 (d 2.69 and 1.77, each d), indicating that the ketone was located at C-6. Cross-peaks between the hydroxymethyl protons and C-3 (d 72.4), C-4 (d 42.4), C-5 (d 59.1) and methyl carbon (d 12.8) indicated that the hydroxymethyl group was located at C-4. In the differential NOE experiment, irradiation of the methyl protons located at C-4 (d 1.06) led to enhancement of the signal intensities of the H-25 methyl protons at d 0.96 (5%) and the hydroxymethyl protons at d 3.51 (3%) and d 3.38 (2%), indicating that the A new oleanan-type triterpene (1) and three new cincholic acid glycosides (2-4) were isolated from Peruvian "Uña de Gato" (Cat's claw, plant of origin: Uncaria tome...

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confidence: 99%

New Oleanan-Type Triterpene and Cincholic Acid Glycosides from Peruvian "Una de Gato" (Uncaria tomentosa)

Kitajima

Hashimoto

Sandoval

et al. 2004

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…Recently, quinic acid (QA) and QA esters have been identified as active anti-inflammatory ingredients in hot water extracts of the herbal Uncaria tomentosa or Cat's claw (e.g., C-MED-100  ), 1,2 and shown to enhance immune cell response and DNA repair in humans. [3][4][5] This chapter will give an overview on the most recent studies on QA and its derivatives in the following fields, such as: biosynthesis and catabolism in nature, synthesis, biological activities, mechanisms of anti-inflammatory activity, etc.…”

Section: Chapter 1 Introductionmentioning

confidence: 99%

“…Recently, QA has been identified as an active inflammatory ingredient in hot water extracts of the herbal Cat's claw (e.g., C-MED-100  ), and shown to enhance immune cell response and DNA repair in humans. [3][4][5] Unfortunately, QA is utilized by gastrointestinal bacteria as a carbon source for aromatic acid synthesis. Consequently, only a small fraction of QA is absorbed after oral administration of QA.…”

Section: Introductionmentioning

confidence: 99%

“…113 Prolonged cell survival has been linked to enhanced DNA repair. [3][4][5] Recently, quinic acid (QA) esters have been identified as biologically active components in the extract. 1 The mechanism by which QA and its esters exert their anti-inflammatory activity is unclear, but appears to be related to inhibition of the pro-inflammatory transcription factor nuclear factor kappa B (NF-B).…”

Section: Introductionmentioning

confidence: 99%

“…[8][9][10] Recently, QA has been identified as an active ingredient in hot water extracts of the herbal Cat's claw (e.g., C-MED-100  ), and shown to enhance immune cell response and DNA repair in humans. [3][4][5] In particular, such extracts have been found to have various anti-inflammatory effects such as inhibition of the production of the inflammatory cytokine TNF, scavenges free radicals 45 and the activation of the central transcription factor nuclear factor κB (NF-κB). [46][47][48][49] Unfortunately, QA is utilized by microorganisms and plants through the shikimate biosynthetic pathway leading to aromatic amino acids phenylalanine, tyrosine, and tryptophan.…”

Section: Introductionmentioning

confidence: 99%

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