2018
DOI: 10.4103/1735-3327.240472
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DNA profiling of Streptococcus mutans in children with and without black tooth stains: A polymerase chain reaction analysis

Abstract: Background:The purpose of this study was to identify the genetic patterns of Streptococcus mutans by DNA finger printing among caries-active, caries-free, and in children with black tooth stains.Materials and Methods:In this in vitro study Ten children with black tooth stains and ten caries-free and caries-active children between 3 and 6 years of age were recruited. Saliva samples were collected using a sterile tube, followed by microbial culture of S. mutans, DNA isolation, and polymerase chain reaction ampli… Show more

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Cited by 10 publications
(9 citation statements)
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References 22 publications
(29 reference statements)
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“…In the second molar Mesio buccal root had Type I canal configuration in 90% of teeth and type IV in 10% teeth. Disto buccal had Type I in 100% of the teeth [3][4][5][6][7][8]. In maxillary molars the mesio buccal root had maximum angulations (18.66°), followed by the disto buccal root (15.40°), palatal root (12.29°) showed the least angulations.…”
Section: Background: Please Site All References In the Text #9 To 13mentioning
confidence: 99%
“…In the second molar Mesio buccal root had Type I canal configuration in 90% of teeth and type IV in 10% teeth. Disto buccal had Type I in 100% of the teeth [3][4][5][6][7][8]. In maxillary molars the mesio buccal root had maximum angulations (18.66°), followed by the disto buccal root (15.40°), palatal root (12.29°) showed the least angulations.…”
Section: Background: Please Site All References In the Text #9 To 13mentioning
confidence: 99%
“…Other goals such as educating parents about proper oral hygiene for infants and toddlers, the use of fluoride, oral habits, teething management, can also be done to maintain a proper oral hygiene of the child (cui et al, 2016). Our department is passionate about child care, we have published numerous high quality articles in this domain over the past 3 years (govindaraju, Jeevanandan and subramanian, 2017a, 2017b; Panchal, gurunathan and shanmugaavel, 2017; Ravikumar, Jeevanandan and subramanian, 2017; Jeevanandan and govindaraju, 2018;nair et al, 2018;Ravikumar et al, 2018Ravikumar et al, , 2019Ravindra et al, 2018, Due to pain (y/n) 2)…”
Section: Introductionmentioning
confidence: 99%
“…hence parents play an important role in the assessment of pain in neonates and toddlers. (reid et al, 1995; The Dental Discomfort questionnaire (DDQ) is a tool devised to recognise pain and to assess the severity of pain in children 2-5 years of age through the reports of parents / caregivers regarding the changes in their child's behaviour. (Knowledge and Attitude of Parents regarding Children's Primary Teeth & their Willingness for Treatment, no date; Versloot, Veerkamp and hoogstraten, 2006;Daher et al, 2014) There are several factors associated with dental pain.…”
Section: Introductionmentioning
confidence: 99%
“…(low, Tan and Schwartz, 1999) our department is passionate about child care, we have published numerous high quality articles in this domain over the past 3 years. Subramanian, 2017a, 2017b;Panchal, Gurunathan and Shanmugaavel, 2017;ravikumar, Jeevanandan and Subramanian, 2017;Jeevanandan and Govindaraju, 2018;nair et al, 2018;ravikumar et al, 2018ravindra et al, 2018Subramanyam et al, 2018;Vishnu Prasad et al, 2018;Jeevanandan, Ganesh and Arthilakshmi, 2019;ramadurai et al, 2019;ramakrishnan, Dhanalakshmi and Subramanian, 2019;Veerale Panchal, Jeevanandan and Subramanian, 2019;Vignesh et al, 2019;Samuel, Acharya and rao, 2020). With this inspiration we planned to pursue research on the factors associated with dental pain in toddlers -using dental discomfort questionnaire (DDQ).…”
Section: Introductionmentioning
confidence: 99%