DNA probes for species and strain identification in the ectomycorrhizal fungus Hebeloma

Marmeisse, Roland; Debaud, Jean‐Claude; Casselton, Lorna A.

doi:10.1016/s0953-7562(09)80960-x

Cited by 29 publications

(10 citation statements)

References 29 publications

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“…(1997 ) is best explained by somatic DNA rearrangements in the DNA sequences which were used to create these subdivisions. These DNA sequences, RC15 and Tel1, were first selected as probes to identify individuals, as they were shown to be hyperpolymorphic ( Marmeisse et al ., 1992 and R. Marmeisse, unpubl. results).…”

Section: Discussionmentioning

confidence: 99%

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Population dynamics of the symbiotic mushroom Hebeloma cylindrosporum: mycelial persistence and inbreeding

Gryta¹,

Debaud²,

Marmeisse³

2000

Heredity

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The pattern of colonization of a forest site by the ectomycorrhizal basidiomycete fungus Hebeloma cylindrosporum Romagnesi was followed from 1993 until 1997. Fruit-bodies of this tetrapolar heterothallic species were mapped, collected and propagated as pure mycelial cultures. Isolates were analysed for their mating-types and molecular markers (rDNA polymorphism and RAPD). Dedikaryotization of the 26 isolates collected in 1993 and the separate analysis of each individual haploid nucleus established that two fully compatible genets, which occupied two nonoverlapping territories, were present. Isolates belonging to the same genet could nevertheless be distinguished from each other based on Southern hybridization using hyperpolymorphic DNA probes. A majority of the 143 isolates collected from 1994 to 1997 belonged to either of the two genets identified in 1993, whose territories extended at a rate of about 0. 45-0.60 m per year. Selfing of the two 1993 genets, rather than outcrossing, was the most likely explanation for the origin of additional genotypes identified between 1995 and 1997. The spatial distribution of fruit-bodies and genets of H. cylindrosporum suggested that only a fraction of the sampled area was favourable to colonization and that genetic diversification through meiospore dispersal may be inhibited by the presence of resident genets, possibly via a somatic incompatibility system.

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Section: Discussionmentioning

confidence: 99%

“…(1997 ). The probes used were the RC15 sequence which identifies a single genomic locus ( Marmeisse et al ., 1992 ) and the Tel1 sequence which identifies a small family of unlinked genomic sequences in the H. cylindrosporum genome (R. Marmeisse, unpubl. data; Gryta et al ., 1997 ).…”

Section: Methodsmentioning

confidence: 99%

Population dynamics of the symbiotic mushroom Hebeloma cylindrosporum: mycelial persistence and inbreeding

Gryta¹,

Debaud²,

Marmeisse³

2000

Heredity

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“…d-e. restriction Sites), have been reported for several species of ectomycorrhizal fungi (Armstrong, Fowles and Rygiewicz, 1989;Rogers et al, 1989;Egger & Fortin, 1990;Gardes et al, 1990;Egger, Danielson & Fortin, 1991;Martin et al, 1991;LoBuglio, Rogers & Wang, 1992;Marmeisse, Debaud & Casselton, 1992). Although this method may be more sensitive than microbiological and immunochemical methods, it still may lack the sensitivity required to determine the ultimate fate of introduced isolates because of the relatively limited number of target genetic sequences that may be present in one ectomycorrhizal root.…”

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confidence: 99%

Rapid identification of genetic variation of ectomycorrhizal fungi by amplification of ribosomal RNA genes

1992

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SUMMARYThere is a clear requirement to develop sensitive methods for detecting defined isolates of ectomycorrhizal fungi within the complex microbial communities of natural ecosystems and reforestation sites. We present a method that permits the rapid identification of an ectomycorrhizal isolate using enzymatic amplification (polymerase chain reaction) of DNA extracted either from pure cultures or ectomycorrhizas. A set of oligonucleotide primers capable of amplifying full-length nuclear 17S and 25S ribosomal RNA genes, together with the ribosomal internal transcribed spacer and intergenic spacer, have been designed and could be used for amplifying target sequences from a wide range of ectomycorrhizal genera. Length polymorphism in the amplified rDNA and restriction endonuclease analysis of nearly 6-0 kbp of amplified rDNA provided useful criteria for the rapid typing of isolates from different genera and species. Restriction endonuclease analysis of amplified DNA from 26 isolates representing four species oi Laccaria (L. bicolor, L. laccata, L. proximo, L. tortilis) yielded up to 20 scored RFLPs and revealed interspecific and intraspecific polymorphism. Most of the polymorphisms were located within the regions corresponding to the internal transcribed spacer and intergenic spacer. The degree of variation observed was sufificient to discriminate several isolates from the same species. Genetic variation was correlated to some extent with geographical origin of the isolates. However, RFLPs of the rRNA genes cannot unambiguously discriminate all selected isolates within Laccaria species, requiring the development of additional DNA probes. Alone, or in combination with other DNA probes, the amplified rDNA genes may serve in the determination of pure fungal cultures and in the characterization of genetic variation of field ectomycorrhizal populations.

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“…The primary technique for constructing DNA fingerprints is RFLP analysis which detects length mutations and alterations in base sequences (i.e. restriction sites) (Armstrong et al, 1989;LoBuglio et al, 1991;Martin et al, 1991;Marmeisse et al, 1992b). However, the inability to detect DNA on a single mycorrhiza and utilization of radioactive isotopes prevented the spread of conventional RFLPs in identifying ectomycorrhizal isolates for characterization of community structures.…”

Section: Identification Of Genetic Variability Of Ectomycorrhizal Funmentioning

confidence: 99%

Genetics of ectomycorrhizal fungi: progress and prospects

1994

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The variability within and among ectomycorrhizal species provides a substantial genetic resource and the potential to increase forest productivity and environmental sustainability. Two parallel and interacting approaches, classical and molecular genetics, are being developed to acquire the genetic information underpinning selection of improved ectomycorrhizal strains. Determining the genetic traits of the fungi which contribute to symbiosis and plant function are being followed using natural variability combined with classical and molecular genetic manipulations. Classical and molecular manipulations for breeding rely on key information including sexual and parasexual reproduction, postmeiotic nuclear behaviour, mating-types and vegetative incompatibility mechanisms. Progress in the manipulation of genomes of ectomycorrhizal fungi will depend on efficient methods for gene cloning and DNA transformation. Gene transfer into fungal cells have been shown to be successful and include treatment of protoplasts and intact mycelium with naked DNA in the presence of polyvalent cations, electroporation, and microbombardment. The merits and limitations of these methods are discussed. Using this technology the expression of foreign DNA, the functional analysis of fungal DNA sequences, as well as molecular exploitation for commercial purposes can be carried out. This review concentrates on these aspects of fungal molecular biology and discusses the applications of the experimental systems that are currently available to ectomycorrhizal fungi. As it is essential to be able to define the traits which a breeder is seeking to improve, availability of genetically defined strains that are isogenic for a character or differ only in one character and a thorough knowledge of the biochemistry of the symbiosis will be necessary before any genetic manipulation be carried out. Genetic variability of ectomycorrhizal strains has been assessed by DNA fingerprinting. This approach allows the evaluation of DNA variability and the exchange of genetic information in natural populations, the identification of species and isolates by DNA polymorphisms, and tracking the environmental fate of the introduced fungi to determine their survival, growth, and dissemination within the soil.

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DNA probes for species and strain identification in the ectomycorrhizal fungus Hebeloma

Cited by 29 publications

References 29 publications

Population dynamics of the symbiotic mushroom Hebeloma cylindrosporum: mycelial persistence and inbreeding

Population dynamics of the symbiotic mushroom Hebeloma cylindrosporum: mycelial persistence and inbreeding

Rapid identification of genetic variation of ectomycorrhizal fungi by amplification of ribosomal RNA genes

Genetics of ectomycorrhizal fungi: progress and prospects

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