DNA Polymorphisms in SREBF1 and FASN Genes Affect Fatty Acid Composition in Korean Cattle (Hanwoo)

Bhuiyan, M. S. A.; Yu, Shanlin; Jeon, Jin-Tae; Yoon, Duhak; Cho, Y. M.; Park, E. W.; Kim, N. K.; Kim, Kang Su; Lee, Jun Heon

doi:10.5713/ajas.2009.80573

Cited by 43 publications

(40 citation statements)

References 34 publications

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“…Han et al (6) found that the frequency of the S allele in the Angus and Charolais crossbred was also marginal (0.01), with the absence of the SS genotype. The estimated frequency of the S allele in Limousin cattle was 0.335, whereas the value reported by Bhuiyan et al (5) was considerably lower (0.22).…”

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confidence: 43%

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Insertion/deletion polymorphism of the sterol regulatory element-binding protein 1 gene in different cattle breeds

Proskura

2014

Turk J Vet Anim Sci

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Sterol regulatory element-binding protein 1 (SREBP1) is the main transcription factor linked to lipogenesis regulation. SREBP1 regulates the expression of the FASN and ACACA genes coding for the 2 major lipogenic enzymes involved in de novo fatty acid synthesis, fatty acid synthase and acetyl-CoA carboxylase, respectively (1). The SREBP1 pathway plays a key role in the regulation of milk fat synthesis, and thus the variation within the SREBP1 gene may influence fat content and especially fatty acid composition in bovine milk and meat (2). Hoashi et al. (3) determined the complete sequence of this gene. They discovered an insertion/deletion (ins/del) of 84 bp in intron 5 of the SREBP1 gene. The short (S)-and long (L)-type alleles were identified. Recently, several studies have indicated an association between this ins/ del polymorphism and fatty acid composition in the milk and meat of cattle (2,4-6). The aim of this study was to analyze the genotype distribution of the above-mentioned polymorphism in cattle populations of different breeds.The study included a total of 662 individuals of the following breeds: Montbeliard, Limousin, HolsteinFriesian, Angus, Jersey, and Charolais. DNA was extracted from whole peripheral blood, collected from the jugular vein into test tubes containing an anticoagulant (K 3 EDTA) using the MasterPure DNA Purification Kit for Blood (Epicentre Biotechnology). The primers used for amplification were those described previously (7). PCR amplifications were performed in a 10-µL volume of reaction mixture containing: approximately 60 ng of genomic DNA, 15 pmol of each primer, 1X Taq DNA polymerase buffer with (NH 4 ) 2 SO 4 , 1.5 mM MgCl 2 , 0.2 mM dNTP, 0.4 U of Taq DNA polymerase, and nucleasefree deionized water up to 10 µL. Amplifications were carried out according to the following temperature profile: initial denaturation at 94 °C for 5 min, followed by 35 cycles (denaturation at 94 °C for 30 s, primer annealing at 56 °C for 45 s, extension at 72 °C for 45 s) and final extension at 72 °C for 5 min. PCR products were separated in a 1.5% agarose gel in 1X TBE buffer and stained with ethidium bromide. After electrophoresis, DNA bands were visualized under UV light and photographed. Two alleles (L and S, corresponding to bands of 492 and 408 bp, respectively) and 3 genotypes (LL, LS, and SS) were identified.The analysis included 6 Polish cattle herds consisting of beef (Limousin, Angus, Charolais), dairy (HolsteinFriesian, Jersey), and dual-purpose (Montbeliard) breeds. Allele and genotype frequencies obtained in the present study are given in the Table. There was no polymorphism in the SREBP1 locus in dairy cattle populations. All HolsteinFriesian and Jersey individuals were LL homozygotes. Kaneda et al. (8) also reported monomorphism inAbstract: In the present study, an 84-bp insertion/deletion polymorphism of the sterol regulatory element-binding protein 1 (SREBP1) gene was investigated. The analysis included 6 Polish cattle herds consisting of beef (Limousin, Angus, Charolais), dairy...

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confidence: 43%

“…The short (S)-and long (L)-type alleles were identified. Recently, several studies have indicated an association between this ins/ del polymorphism and fatty acid composition in the milk and meat of cattle (2,(4)(5)(6). The aim of this study was to analyze the genotype distribution of the above-mentioned polymorphism in cattle populations of different breeds.…”

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confidence: 99%

Insertion/deletion polymorphism of the sterol regulatory element-binding protein 1 gene in different cattle breeds

Proskura

2014

Turk J Vet Anim Sci

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“…Furthermore, in a study of five different breeds, this polymorphism was only found in the Limousine breed, suggesting that it might be a breed-specific phenomenon and may not be segregating widely in different cattle populations (Bhuiyan et al, 2009). In the present study, Snow Dragon black cattle showed a slightly higher frequency of the SS genotype compared to Simmental bulls, which may be due to its long history of selective breeding.…”

Section: Discussionmentioning

confidence: 45%

“…In contrast, the stearic acid concentration was 6.30% lower in the SS genotype compared to the LL genotype (P < 0.05), but the linoleic and PUFA contents were 11.06 and 12.20% higher in SS compared to LL (Bhuiyan et al, 2009). Several studies have reported associations between SREBP1 and fatty acid composition and carcass traits.…”

Section: Discussionmentioning

confidence: 84%

Polymorphism of SREBP1 is associated with beef fatty acid composition in Simmental bulls

Liu¹,

Zhang²,

Yuan³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The sterol regulatory element binding factor 1 gene (SREBP1) plays an important role in the biosynthesis of fatty acids and cholesterol, and in lipid metabolism. The objective of this study was to investigate the effect of genetic polymorphisms of SREBP1 on the fatty acid composition of muscle and carcass traits in Simmental bulls and Snow Dragon black cattle. The 84-bp insertion/deletion (indel) in intron 5 of the bovine SREBP1 gene was genotyped by polymerase chain reaction to investigate its associations with traits. The results showed that the 84-bp indel in intron 5 was significantly associated with palmitoleic acid (C16:1), stearic acid (C18:0), saturated fatty acids (SFA), triglycerides (TAG), and the C16 index in Simmental bulls (P < 0.05). Cattle with the LL genotype had higher palmitic acid (C16:1), triglycerides, and C16 index but lower stearic acid (C18:0) and SFA compared to those with the LS genotype (P < 0.05). In conclusion, the 84-bp indel of SREBP1 could be used as a genetic marker for selecting Simmental breeding stock for healthier fatty acid composition.

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“…The FASN gene has seven active sites that help to catalyze all the reaction steps in the conversion of acetyl-CoA and malonyl-CoA to palmitate (Roy et al, 2005). Several reports indicated that the mutations in FASN gene were associated with (Bhuiyan et al, 2009;Lee et al, 2010;Zhang et al, 2008). Also, the polymorphisms in chicken FASN gene have been reported by Marrube et al (2004) and this gene is located on chromosome 18 and had three TE domains in exons 40, 41 and 42 (Kasturi et al, 1988).…”

Section: Introductionmentioning

confidence: 95%

Association of FASN and SCD genes with fatty acid composition in broilers

Maharani¹,

Seo²,

Choi³

et al. 2013

Korean Journal of Agricultural Science

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: Fatty acids (FAs) were considered in activating nuclear hormone receptors that play significant roles in the cellular lipid metabolism by the regulation of several genes. Previously, fatty acid synthase (FASN) and stearoyl-CoA desaturase (SCD) genes have been known to regulating the FA metabolism. In this study, associations of FASN and SCD genes with fatty acid (FA) composition in broilers were investigated. Tissue samples from 95 Cobb 500 broilers were used for DNA extraction. The g.1222 A>G SNP located in intron 42 of FASN gene and 2 SNPs in SCD gene, one in exon 2 (g.3728A>G) and the other in exon 4 (g.12903G>A), were subjected for genotyping using PCR-RFLP method. One of the SNPs in SCD gene, SNP g.3728A>G had significant association with myristoleic acid (C14:1; P<0.05), palmitic acid (C16:0; P<0.05), palmitoleic acid (C16:1; P<0.05) and saturated FA (SFA; P<0.05). However, the SNP g.1222A>G in FASN gene had only suggestive association with arachidic acid (C20:0; P=0.08). The findings in this study suggest that the SNP in exon 2 of SCD gene can be used as a molecular marker for selecting birds having desirable FA composition in broilers.

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DNA Polymorphisms in SREBF1 and FASN Genes Affect Fatty Acid Composition in Korean Cattle (Hanwoo)

Cited by 43 publications

References 34 publications

Insertion/deletion polymorphism of the sterol regulatory element-binding protein 1 gene in different cattle breeds

Insertion/deletion polymorphism of the sterol regulatory element-binding protein 1 gene in different cattle breeds

Polymorphism of SREBP1 is associated with beef fatty acid composition in Simmental bulls

Association of FASN and SCD genes with fatty acid composition in broilers

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