DNA methylation in early development

Geiman, Theresa M.; Muegge, Kathrin

doi:10.1002/mrd.21118

Cited by 117 publications

(96 citation statements)

References 86 publications

(108 reference statements)

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“…2D), but at 1 week the cases of ICF (immunodeficiency, centromeric instability and facial anomalies) syndrome, a rare autosomal recessive disease. 6,7 Marked hypomethylation and decondensation of pericentromeric heterochromatin on specific chromosomes have been reported in adult patients with ICF syndrome, 2,6,7 suggesting that Dnmt3b plays a critical role in DNA methylation and genomic stability in adults as well as in embryos.…”

Section: Diurnal Variations Inmentioning

confidence: 99%

“…To investigate the mechanism, we (1) compared the Dnmt3b transcription pattern to the transcription patterns of circadian pacemaker genes, (2) conducted an experiment to analyze is intended to separate the feeding cycle from the light/dark cycle, should lead to a phase-shift in the Dnmt3b mRNA expression pattern. We therefore compared Dnmt3b mRNA expressions at ZT 0, 4, 8, 12 and 16 in an ad-lib fed group and SF group.…”

Section: Diurnal Variations Inmentioning

confidence: 99%

“…DNA methylation is an essential epigenetic machinery that suppresses gene expression via transcription silencing at gene promoters and repetitive sequences and is considered to be important not only in early development 1,2 but also in the pathogenesis of diseases, such as cancer, diabetes and stress disorder in adults. 1,3,4 The cytosine that is present in abundance in CpG islands of genomic DNA can be converted to 5-methyl-cytosine (5-mC) by DNA methyltransferase (DNMT).…”

Section: Introductionmentioning

confidence: 99%

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Diurnal expression ofDnmt3bmRNA in mouse liver is regulated by feeding and hepatic clockwork

et al. 2012

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Section: Diurnal Variations Inmentioning

confidence: 99%

Section: Diurnal Variations Inmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Diurnal expression ofDnmt3bmRNA in mouse liver is regulated by feeding and hepatic clockwork

et al. 2012

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“…DNA methylation is one of the most important mechanisms of mammalian epigenetics and regulates cell differentiation as well as individual development by affecting the stability of the chromosome structure, the selectivity of gene expression, and DNA replication, recombination and repair. 12,13 Finally, we also observed increased expressions of parathyroid hormone, cyclin A1-interacting protein 1 and meiosis-expressed gene-1-like genes, suggesting the disease complexity of DCS.…”

Section: Discussionmentioning

confidence: 59%

Gene expression profile of type II spinal cord decompression sickness

et al. 2014

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Study design: This study was an experimental, controlled, animal study. Objective: This study was to determine the changes of molecular pathology in spinal cord decompression sickness (SC-DCS) based on a rabbit model of SC-DCS with the aid of an all-gene expression profile chip. Setting: Qingdao, Shandong Province, China. Methods: A gene expression profile chip containing 43 803 genes was used to compare the gene expressions in the spinal cords of four male New Zealand white rabbits in the SC-DCS and control groups, respectively. Selected differentially expressed genes were identified with quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry. Results: The chip hybridization results showed that the SC-DCS group had nine upregulated and seventeen downregulated genes, compared with the control group. These genes were mainly related to inflammation, ion channels, the cell cycle, material transfer and apoptosis. The qRT-PCR results showed that parathyroid hormone and tumor necrosis factor alpha (TNF-a) genes were upregulated compared with the control group (Po0.01). However, the acyl-CoA synthetase and voltage-gated channel genes were downregulated (Po0.05). The immunohistochemical staining results confirmed that there were significantly greater expression levels of TNF-a in the spinal cord tissues of the SC-DCS group compared with the control group. Conclusions: The spinal cord lesions of SC-DCS involve multiple gene changes in the rabbit; however, the significance of these findings needs further research. Meanwhile, the gene expression profile chip results provide us with a better understanding of the pathogenesis of DCS.

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“…Epigenetic programing predetermines the developmental program and provides necessary direction for the multitude of changes that are required to proceed from a fertilized oocyte to a fully developed adult animal (1). It has been suggested that epigenetic changes are associated with development and differentiation.…”

Section: Introductionmentioning

confidence: 99%

DNA hypomethylation at the ZNF206-exon 5 CpG island associated with neuronal differentiation in mice and development of neuroblastoma in humans

Kawashima

Sugito²,

Yoshizawa³

et al. 2011

Int J Oncol

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Abstract. Differentiation of human neuroblastoma recapitulates neural crest development. In our whole genome DNA methylation screening of tissue-specific differentially methylated regions (T-DMRs) and developmental stage specific differentially methylated regions (DS-DMRs) we reported that the exon 5 CpG island (CpGi) of Zfp206 (human: ZNF206), which was required to maintain embryonic stem cells in a pluripotent state, was one of potent brain and testis-specific T-DMRs in mice. In this study methylation level of the CpG sites at Zfp206-exon 5 CpGi in mouse brain samples at three different developmental stages (15-day-old embryo; E15, new born; NB, 12-week adult; AD) were quantitatively analyzed and it was identified that Zfp206-exon 5 CpGi was the DS-DMRs in mouse brain. In AD brains, Zfp206-exon 5 CpGi was significantly hypomethylated and Zfp206 expression was repressed, compared with E15 and NB brains. Hence, mehtylation level of human 5'-end of CpGi at ZNF206-exon 5, which is homologous CpGi to mice, was analyzed in neuroblastomas. Although all four adrenal samples showed complete methylation at the homologous region, we found the hypomethylation in 7 out of 26 neuroblastomas and a significant association between the hypomethylation and poor prognosis. In neuroblastoma cell lines and specimens, the hypomethylation was also associated with ZNF206 expression. These data indicated that the changes in DNA methylation levels at the Zfp206-exon 5 might be one of the important factors during neuronal development in mice and that the hypomethylation of the homologous region induced ZNF206 expression in humans and was associated with human neuroblastomagenesis. Even though the function of ZNF206 and its expression regulation in neuroblastoma remain elusive, ZNF206 might be a candidate differentiation suppressor and prognosis marker in neuroblastoma. IntroductionEpigenetic programing predetermines the developmental program and provides necessary direction for the multitude of changes that are required to proceed from a fertilized oocyte to a fully developed adult animal (1). It has been suggested that epigenetic changes are associated with development and differentiation. DNA methylation is one of the epigenetic factors and plays an important role in the diverse genomic process, such as gene regulation, chromosomal stability, parental imprinting and X-inactivation (2). Recent genome-wide DNA methylation searches indicate that 4-17% of CpG sites are different in methylation among tissues and developmental processes (3,4). Tissue-specific differentially methylated regions (T-DMRs) and developmental-specific differentially methylated regions (DS-DMRs) are suggested to play important roles in development and differentiation (5). Therefore, disruption of epigenetic processes can lead to altered gene function and malignant cellular transformation (6). For example, Hox genes are involved in determining anterior-posterior embryonic pattern and govern DNA hypomethylation at the ZNF206-exon 5 CpG island associated with neuronal ...

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DNA methylation in early development

Cited by 117 publications

References 86 publications

Diurnal expression ofDnmt3bmRNA in mouse liver is regulated by feeding and hepatic clockwork

Diurnal expression ofDnmt3bmRNA in mouse liver is regulated by feeding and hepatic clockwork

Gene expression profile of type II spinal cord decompression sickness

DNA hypomethylation at the ZNF206-exon 5 CpG island associated with neuronal differentiation in mice and development of neuroblastoma in humans

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