DNA‐Mediated Proximity‐Based Assembly Circuit for Actuation of Biochemical Reactions

Abstract

Smart nanodevices that integrate molecular recognition and signal production hold great promise for the point-of-care (POC) diagnostic applications. Herein, the development of a DNA-mediated proximity assembly of biochemical reactions, which was capable of sensing various bio-targets and reporting easy-to-read signals is reported. The circuit was composed of a DNA hairpin-locked catalytic cofactor with inhibited activity. Specific molecular inputs can trigger a conformational switch of the DNA locks through the mechanisms of toehold displacement and aptamer switching, exposing an active cofactor. The subsequent assembly of an enzyme/cofactor pair actuated a reaction to produce colorimetric or fluorescence signals for detecting target molecules. The developed system could be potentially applied to smart biosensing in molecular diagnostics and POC tests.

RNA‐Cleaving DNA Thresholder Controlled by Concentrations of miRNA Cancer Marker

“…More recently, Oh et al. used a DNA hairpin‐based circuit, which was capable of sensing various biological molecules and reporting the output via easy‐to‐read signals [5] . Gong et al.…”

“…[1] More recently, Oh et al used a DNA hairpin-based circuit, which was capable of sensing various biological molecules and reporting the output via easyto-read signals. [5] Gong et al realized multiple endogenous miRNA-initiated biocomputing operations for cell recognition. [6] We have been developing DNA logic gates for detecting complex patterns of nucleic acids [7] with the final goal of building a DNA nanorobot for biomarker-dependent treatment of human diseases.…”

A Janus-inspired amphichromatic system that kills two birds with one stone for operating a “DNA Janus Logic Pair” (DJLP) library

“…It is recognized that versatile logic systems can provide intelligent solutions to various bio-applications such as logicprogrammed biosensing/bio-diagnosis. [42][43][44][45][46][47][48]63 Antioxidants (such as GSH) play signicant roles in many physiological functions such as helping to trap and neutralize the free radicals, thereby eliminating their harmful effects on the human body. The alternation in the level of antioxidants has been associated with many diseases, thus making the recognition of their absence/presence more signicant.…”

Nucleic-Acid Driven Cooperative Bioassays Using Probe Proximity or Split-Probe Techniques

“…Among the many advancements in biosensing over the last few decades, a number of powerful and practically useful assays have been devised by mimicking binding and recognition schemes already present in nature. One particularly clever means of analyte sensing is to leverage cooperative binding of multiple probes to a targeted analyte molecule. − Where simple optical ,, or electrochemical − readouts can be used to quantify this cooperative complex, various protein or small molecule analytes can be selectively detected with speed, sensitivity, and minimized assay workflow.…”