DNA-mediated Au@Ag@silica nanopopcorn fluorescent probe for in vivo near-infrared imaging of probiotic Lactobacillus Plantarum

Gao, Fei; Zhou, Shuaishuai; Lu, Chang; Li, Yuzhi; Lin, Jianhan; Zheng, Jinkai

doi:10.1016/j.bios.2022.114421

Cited by 3 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Second, the luciferase gene is inserted into the chromosome of the intended bacterium and is stably and consistently expressed, but luminescence can only be achieved when the exogenous substrate luciferin is administered. [10,11] For exogenous bioluminescence-based [ [48][49][50][51] Smart-activated imaging agents The imaging agent is cut by bacterial enzymes, resulting in the removal of the quencher and a detectable fluorescent signal.…”

Section: Bioluminescence-based Imagingmentioning

confidence: 99%

“…For example, Liu et al [49] used sequence-specific DNA molecules to modify persistent luminescent nanophosphorescent materials with the advantages of having ultra-long afterglow and exhibiting excellent biocompatibility and stability for real-time tracking of Lactobacillus casei in the gut. Gao et al [50] synthesized a DNA-mediated Au@Ag@silica nanofluorescent probe for high-performance NIR imaging of probiotics in vivo. Recently, Zhang et al [51] developed a Trojan-bioluminescent probe (Trojan-BLI), which combined luciferase and a substrate using glucose polymer-modified nanocarriers.…”

Section: Optical Imaging Of Nanomaterialsmentioning

confidence: 99%

See 1 more Smart Citation

Advances in Bio‐Optical Imaging Systems for Spatiotemporal Monitoring of Intestinal Bacteria

Pan,

Su,

Zhang

et al. 2024

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Vast and complex intestinal communities are regulated and balanced through interactions with their host organisms, and disruption of gut microbial balance can cause a variety of diseases. Studying the mechanisms of pathogenic intestinal flora in the host and early detection of bacterial translocation and colonization can guide clinical diagnosis, provide targeted treatments, and improve patient prognosis. The use of in vivo imaging techniques to track microorganisms in the intestine, and study structural and functional changes of both cells and proteins, may clarify the governing equilibrium between the flora and host. Despite the recent rapid development of in vivo imaging of intestinal microecology, determining the ideal methodology for clinical use remains a challenge. Advances in optics, computer technology, and molecular biology promise to expand the horizons of research and development, thereby providing exciting opportunities to study the spatio‐temporal dynamics of gut microbiota and the origins of disease. Here, this study reviews the characteristics and problems associated with optical imaging techniques, including bioluminescence, conventional fluorescence, novel metabolic labeling methods, nanomaterials, intelligently activated imaging agents, and photoacoustic (PA) imaging. It hopes to provide a valuable theoretical basis for future bio‐intelligent imaging of intestinal bacteria.

show abstract

Section: Bioluminescence-based Imagingmentioning

confidence: 99%

Section: Optical Imaging Of Nanomaterialsmentioning

confidence: 99%

Advances in Bio‐Optical Imaging Systems for Spatiotemporal Monitoring of Intestinal Bacteria

Pan,

Su,

Zhang

et al. 2024

Molecular Nutrition Food Res

View full text Add to dashboard Cite

show abstract

Marigold Like Structure from Methionine Mediated Growth of Positively Charged Gold Nanorod

Sahu,

Sadhu

2024

ChemNanoMat

View full text Add to dashboard Cite

During morphological evolution of gold nanoparticles, amino acids play a vital role in tuning shape, introducing chirality and inducing facet selective reactivity. Herein, we report the synthesis of unique marigold like structure (MGS) via growth reaction of methionine (Met) incubated positively charged anisotropic gold nanorod (GNR). Varying three important parameters such as growth time, concentration of Met and Au3+ reveals the combination of freshly generated small nucleated particles (fNPs) and GNR to form the centrally accumulated gold as the disk floret design of MGS.Strong interaction between Met and (111) plane of Au0 controls the orientation of (111) plane parallel to the direction of growth. This preferential interaction directs the assembly of gold nanostructures through Au (200) plane and results in merging of fNPs with concave GNR (cGNR) to fabricate the external arrangement of ray floret structure. The structural selectivity is attributed to the electron donating capacity of thioether functional group of Met(S) to Au+, generated prior to secondary nucleation. As confirmed by XPS and z‐potential analysis, the above interaction controls the Met concentration dependent inhibition of further Au+→Au0 reduction. The growth strategy of GNR has been further validated with a Met enriched peptide to produce disk and ray florets.

show abstract

Biosensors for point-of-care testing and personalized monitoring of gastrointestinal microbiota

Ngashangva

Chattopadhyay

2023

Front. Microbiol.

View full text Add to dashboard Cite

The gastrointestinal (GI) microbiota is essential in maintaining human health. Alteration of the GI microbiota or gut microbiota (GM) from homeostasis (i.e., dysbiosis) is associated with several communicable and non-communicable diseases. Thus, it is crucial to constantly monitor the GM composition and host–microbe interactions in the GI tract since they could provide vital health information and indicate possible predispositions to various diseases. Pathogens in the GI tract must be detected early to prevent dysbiosis and related diseases. Similarly, the consumed beneficial microbial strains (i.e., probiotics) also require real-time monitoring to quantify the actual number of their colony-forming units within the GI tract. Unfortunately, due to the inherent limitations associated with the conventional methods, routine monitoring of one’s GM health is not attainable till date. In this context, miniaturized diagnostic devices such as biosensors could provide alternative and rapid detection methods by offering robust, affordable, portable, convenient, and reliable technology. Though biosensors for GM are still at a relatively preliminary stage, they can potentially transform clinical diagnosis in the near future. In this mini-review, we have discussed the significance and recent advancements of biosensors in monitoring GM. Finally, the progresses on future biosensing techniques such as lab-on-chip, smart materials, ingestible capsules, wearable devices, and fusion of machine learning/artificial intelligence (ML/AI) have also been highlighted.

show abstract

DNA-mediated Au@Ag@silica nanopopcorn fluorescent probe for in vivo near-infrared imaging of probiotic Lactobacillus Plantarum

Cited by 3 publications

References 44 publications

Advances in Bio‐Optical Imaging Systems for Spatiotemporal Monitoring of Intestinal Bacteria

Advances in Bio‐Optical Imaging Systems for Spatiotemporal Monitoring of Intestinal Bacteria

Marigold Like Structure from Methionine Mediated Growth of Positively Charged Gold Nanorod

Biosensors for point-of-care testing and personalized monitoring of gastrointestinal microbiota

Contact Info

Product

Resources

About