DNA index determination with Automated Cellular Imaging System (ACIS) in Barrett's esophagus: Comparison with CAS 200

Huang, Qin; Yu, Chunhao; Klein, Michael A.; Fang, James C.; Goyal, Raj K.

doi:10.1186/1472-6890-5-7

Cited by 29 publications

(54 citation statements)

References 24 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This finding does not correlate well with the results of a recent ICDA study on paraffin BE biopsy sections, which showed aneuploidy in 13% of ND, 60-73% of LGD/HGD, and 100% of EAC cases (Fang et al, 2004). This discrepancy could be due to differences of standardization of the image cytometry methods to determine aneuploidy (i.e., different cutoff values to define aneuploidy, different image cytometry systems), the use of tissue section (overlapping and truncated nuclei) instead of brush cytology or an observer variation when classifying the grade of dysplasia in BE (Huang et al, 2005). Brush cytology has potential advantages over endoscopic biopsy for diagnosing BE and monitoring for dysplasia since it has the ability to sample a greater area of the BE mucosa surface, and may therefore detect lesions that are missed by biopsy protocols.…”

Section: Discussionmentioning

confidence: 74%

Assessment of chromosomal gains as compared to DNA content changes is more useful to detect dysplasia in Barrett's esophagus brush cytology specimens

Rygiel

Milano

Kate

et al. 2008

Genes Chromosomes & Cancer

View full text Add to dashboard Cite

Abnormal DNA ploidy status has been suggested as a prognostic factor for Barrett's esophagus progression into esophageal adenocarcinoma (EAC). The aim of the study was to compare image cytometry DNA analysis (ICDA) and fluorescent in situ hybridization (FISH) in the assessment of DNA ploidy status in Barrett's esophagus (BE), and to determine the value of these abnormalities as an adjunct to conventional cytology in detection of dysplasia and EAC. Brush cytology specimens of 90 BE patients were examined using ICDA and FISH with peri-centromeric probes for chromosomes 7 and 17. The results of ICDA and FISH were compared with each other, and with dysplasia grade or EAC as determined by histology and cytology. FISH and ICDA detected abnormalities in 41% (37/90) and 22% (19/90) of the BE cases, respectively. Gains of chromosome 7 and/or 17 were present in 13% of nondysplasia cases, which further increased with dysplasia stage, while overall DNA content aneuploidy was detected predominantly in high grade dysplasia (HGD) and EAC. Using FISH results combined with cytology, we were able to identify IND/LGD (indefinite/ low grade dysplasia) with a sensitivity and specificity of 75 and 76%, respectively. FISH alone detected HGD/EAC with a high sensitivity and specificity of 85 and 84%, which was superior to that of cytology alone. Thus, FISH is more sensitive than ICDA to detect chromosomal abnormalities in BE brush cytology specimens. FISH detects chromosomal gains in early stages of BE and represents a valuable adjunct to conventional cytology to detect dysplasia or EAC.

show abstract

Section: Discussionmentioning

confidence: 74%

Assessment of chromosomal gains as compared to DNA content changes is more useful to detect dysplasia in Barrett's esophagus brush cytology specimens

Rygiel

Milano

Kate

et al. 2008

Genes Chromosomes & Cancer

View full text Add to dashboard Cite

show abstract

“…12 Briefly, Feulgen-stained slides were automatically scanned. Only the uniformly stained slides were included.…”

Section: Histological Evaluationmentioning

confidence: 99%

“…12 Analysis of the DNA Histograms DNA histograms are plots of DI values vs the number of cells. The presence of peaks of cells with similar DI values and distribution of cells along the histogram were identified.…”

Section: Histological Evaluationmentioning

confidence: 99%

High-fidelity DNA histograms in neoplastic progression in Barrett's esophagus

Zhang

Huang

et al. 2007

Laboratory Investigation

Self Cite

View full text Add to dashboard Cite

This study describes the high-fidelity DNA histograms in different stages of neoplastic progression to Barrett's adenocarcinoma (BAC). High-fidelity DNA histograms were obtained with image cytometry on sections, and were classified based on DNA index values of the peaks into diploid, mild aneuploid, moderate aneuploid and severe aneuploid. Heterogeneity index (HI) representing cells with different DNA content and the 5N exceeding cell fraction were determined. One hundred and eighty-seven cases, including 34 normal gastrointestinal mucosa (control), 66 Barrett'sspecialized intestinal metaplasia (SIM), 22 low-grade dysplasia (LGD), 22 high-grade dysplasia (HGD) and 43 BAC were investigated. Controls showed sharp diploid peaks with HI values less than 13, and no 5N exceeding nuclei. SIM showed a spectrum of histograms including diploid, mild aneuploid and moderate aneuploid histograms. The frequency and severity of aneuploidy increased with worsening histological grades of dysplasia. All BAC cases were aneuploid, with moderate or severe aneuploidy. Marked elevated HI values (420) and 5N exceeding fractions (45%) were found in 5%, 32%, 50% and 88% of cases with SIM, LGD, HGD and BAC, respectively. The high-fidelity DNA histograms suggest that (1) Barrett's SIM may already be dysplastic in nature, and all BAC may be markedly aneuploid; and (2) elevated cellular DNA heterogeneity and 5N fractions may be markers of progressive chromosomal changes and 'unstable aneuploidy' that identifies progressive lesions.

show abstract

“…[2][3][4][5][6][7][8][9][10] Various attempts have been made to automate this diagnostic process of nuclear DNA content assessment using computeraided analysis of two-dimensional (2-D) slides. [9][10][11] Currently, the two most common techniques used to quantify DNA content are flow cytometry (FCM) and 2-D image cytometry (ICM). In FCM, the sample is stained with a DNA specific fluorophore [usually diamidino-2-phenylindole (DAPI)]; the cells rapidly flow one-by-one across the focused laser beam and the intensities of the fluorescences emitted are used to quantify the DNA content.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional DNA image cytometry by optical projection tomographic microscopy for early cancer diagnosis

Agarwal

Biancardi

Patten³

et al. 2014

J. Med. Imag

View full text Add to dashboard Cite

Abstract. Aneuploidy is typically assessed by flow cytometry (FCM) and image cytometry (ICM). We used optical projection tomographic microscopy (OPTM) for assessing cellular DNA content using absorption and fluorescence stains. OPTM combines some of the attributes of both FCM and ICM and generates isometric highresolution three-dimensional (3-D) images of single cells. Although the depth of field of the microscope objective was in the submicron range, it was extended by scanning the objective's focal plane. The extended depth of field image is similar to a projection in a conventional x-ray computed tomography. These projections were later reconstructed using computed tomography methods to form a 3-D image. We also present an automated method for 3-D nuclear segmentation. Nuclei of chicken, trout, and triploid trout erythrocyte were used to calibrate OPTM. Ratios of integrated optical densities extracted from 50 images of each standard were compared to ratios of DNA indices from FCM. A comparison of mean square errors with thionin, hematoxylin, Feulgen, and SYTOX green was done. Feulgen technique was preferred as it showed highest stoichiometry, least variance, and preserved nuclear morphology in 3-D. The addition of this quantitative biomarker could further strengthen existing classifiers and improve early diagnosis of cancer using 3-D microscopy.

show abstract

DNA index determination with Automated Cellular Imaging System (ACIS) in Barrett's esophagus: Comparison with CAS 200

Cited by 29 publications

References 24 publications

Assessment of chromosomal gains as compared to DNA content changes is more useful to detect dysplasia in Barrett's esophagus brush cytology specimens

Assessment of chromosomal gains as compared to DNA content changes is more useful to detect dysplasia in Barrett's esophagus brush cytology specimens

High-fidelity DNA histograms in neoplastic progression in Barrett's esophagus

Three-dimensional DNA image cytometry by optical projection tomographic microscopy for early cancer diagnosis

Contact Info

Product

Resources

About