DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis

Abstract

Abstract:This study aims to report the amplification of the DNA of Leishmania (V.) braziliensis, using polymerase chain reaction, obtained from the saliva of a patient with American cutaneous leishmaniasis who did not present any lesion in the oral mucosa. Amplification produced fragments of 103 bp, an estimated size employing Leishmania (V.) braziliensis primers (b1 e b2). The present results revealed, for the first time, that the in vitro amplification of Leishmania DNA using samples from the salivary fluid of a patient with American cutaneous leishmaniasis is possible. However, more studies are required with a larger number of participants to evaluate the usefulness of saliva as a non-invasive sample for PCR. The development of such non-invasive technique is necessary for the diagnosis of many diseases in the future, especially infectious and parasitic ones.

Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantumin Mato Grosso do Sul, Brazil

“…PCR assay but negative in the PCR assay for L. donovani complex could be infected by other Leishmania spp. species, i.e., L. braziliensis or L. amazonensis, which have been reported previously in the state of Mato Grosso do Sul (DORVAL et al, 2006;CORVALAN et al, 2011).…”

“…(VEXENAT et al, 1996;LUCIANO et al, 2009). In our study, cross-reactivity between dog serum samples positive to T. cruzi and L. braziliensis could not be discarded, since the occurrence of T. cruzi and L. braziliensis among dogs is documented in the state of Mato Grosso do Sul (POMPILIO et al, 2005;SAVANI et al, 2009;SOUZA et al, 2009;CORVALAN et al, 2011). One dog that tested positive in the Leishmania spp.…”

Consecutive Cutaneous and Visceral Leishmaniasis Manifestations Involving a Novel Leishmania Species in Two HIV Patients in Thailand

“…[27][28][29] Among new world leishmaniasis, one report showed L. braziliensis DNA in the saliva of a patient with ATL without clinical visceral or mucosal infection. 30 Tonsils and adenoids have been identified as sites suspected of containing macrophage-engulfed Leishmania. 17,27 Although this study identified DNA of Leishmania in oral secretions, the transmission of the disease from person to person by way of the upper respiratory and alimentary tracts has to be further studied.…”

Detection of Leishmania siamensis DNA in Saliva by Polymerase Chain Reaction

“…[18][19][20] Collection of saliva is noninvasive and convenient for field studies. We describe a PCR to amplify the internal transcribed spacer1 (ITS1) gene of L. siamensis from six infected patients and compare it with specimens collected from patients and different clinical presentations.…”

Detecção De Leishmania (V.) Braziliensis Através De QPCR Em Amostras De Saliva De Pacientes Do Estado De Pernambuco, Área Endêmica Para Leishmaniose Tegumentaramericana

“…O método por fluido salivar demonstra ser então uma alternativa válida para o diagnóstico de Leishmaniose, podendo ser aplicado em situações de trabalho de campo, por exemplo, que requer uma coleta rápida e de fácil manuseio, ou ainda em pediatria devido à dificuldade na coleta de amostras em crianças. Além disto, por ser um fluido orgânico dispensando em um recipiente estéril a obtenção é de baixo custo e em termos de biossegurança o risco de contaminação é praticamente inexistente, como descrito por (CORVALAN et al, 2011). Esta porcentagem elevada de 70% de positividade para indivíduos do sexo masculino pode-se explicar pela atividade agrícola predominante na localidade do estado, áreas endêmicas de LTA (BRITO et al, 2012).…”