DNA-flow cytometry of squamous cell carcinomas from thehuman uterine cervix: The identification of prognostically different subgroups

Rutgers, Derk H.; Linden, Pieter M. van der; Peperzeel, H.A. van

doi:10.1016/s0167-8140(86)80036-6

Cited by 38 publications

(8 citation statements)

References 18 publications

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“…Somewhat in contrast to these results is study of 112 Stage IB-IV cervical cancer patients reported by Rutgers et a1. 126 This cohort was also treated uniformly by radiotherapy. A nonsignificant survival trend favoring aneuploid over diploid and tetraploid patients was observed when all patients were compared.…”

Section: Endometrial Adenocarcinomamentioning

confidence: 99%

Ploidy, proliferative activity and prognosis. DNA flow cytometry of solid tumors

Merkel

McGuire

1990

Cancer

269

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Flow cytometric study has been used to measure the cellular DNA content of solid tumors for the last decade, and of paraffin-embedded tumor specimens for the last 5 years. Ploidy and proliferative activity are the two properties commonly measured by DNA content flow cytometric study. The ability to study archival, paraffin-embedded tumors has hastened an appreciation of the prognostic utility of this assay. Either abnormal ploidy or elevated proliferative activity predict a worsened disease-free or overall survival in most common adult malignancies. Both abnormalities are associated with poor outcome in locoregional breast, non-small cell lung, and colorectal cancers, and in all stages of ovarian cancer. Abnormal ploidy is also a dire prognostic indicator for cancers arising from the kidney, bladder, prostate, and endometrium. Clinical management of patients with these diseases may be aided by studying their tumors for these objective markers of biological aggressiveness.

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Section: Endometrial Adenocarcinomamentioning

confidence: 99%

Ploidy, proliferative activity and prognosis. DNA flow cytometry of solid tumors

Merkel

McGuire

1990

Cancer

269

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“…Rutgers et al (27) reported a poorer prognosis for diploid tumors, particularly in younger patients. In contrast, Jakobsen (2s) found significantly better shortterm survival times and less frequent recurrences for diploid tumors.…”

Section: Discussionmentioning

confidence: 98%

DNA flow cytometry in stage IB and II cervical carcinoma

Pfisterer¹,

Kommoss²,

Sauerbrei³

et al. 1996

Int J Gynecol Cancer

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In a retrospective study the prognostic significance of nuclear DNA content was investigated, as measured by flow cytometry, of the tumor specimens from 212 women with nonpretreated FIGO stage IB and II cervical cancer. One‐hundred and thirty cases (62%) were found to be diploid, whereas 82 (38%) were aneuploid. Univariate analysis of the follow‐up data showed an increased relative risk (RR) for recurrence free survival (RFS) for stage II tumors (RR = 1.87, 95% CI: 1.13–3.10, P = 0.015) and for age (RR = 1.52, 95% CI: 0.66–3.52 and RR = 2.35, 95% CI: 1.19–4.65, P = 0.032). Ploidy showed a relative risk of 1.33 (95% CI: 0.83–2.13, NS). In addition, univariate analysis of overall survival (OS) revealed similar results. For the subgroup of patients with primary surgery (n = 151), positive pelvic nodes (RR = 5.38, 95% CI: 2.70–10.71, P = 0.0001) and parametrial extension (RR = 2.53, 95% CI: 1.24–5.17, P = 0.011) were significant factors for OS after univariate analysis, the estimated effects on RFS were slightly smaller. Multivariate analysis of RFS for the whole study population showed age, histologic grade and stage with a slightly increased risk, but no effect was significant. Ploidy with an RR of 0.97 (95% CI: 0.58–1.62) seems to have no influence on prognosis. For the subgroup with primary surgery, ploidy again failed statistical significance with an RR of 1.20 (95% CI: 0.58–2.49). Our results suggest that abnormalities of the nuclear DNA content in this homogeneous group of patients are associated with clinical and morphological prognosticators, however, ploidy is not an independent prognostic factor for RFS, or for the whole study population or for the subgroup with primary surgery.

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“…The greatest, prospective study (n = 465) could not detect any significance of DNA-ploidy [32]. In studies that found a prognostic significance of DNA-ploidy, DNA-aneuploid tumors [8] but also DNA-diploid ones [33] were identified to be unfavourable. In most studies tumors with low S-phase fraction showed good prognosis in comparison to high proliferating tumors [30,34], but this could not be confirmed in early invasive cervical cancers [31].…”

Section: Prognostic Significance Of Dna-parameter In Comparison To CLmentioning

confidence: 91%

Evaluation of Prognostic Factors Following Flow‐Cytometric DNA Analysis after Cytokeratin Labelling: II. Cervical and Endometrial Cancer

Wimberger

Hillemanns

Kapsner

et al. 2002

Analytical Cellular Pathology

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In gynecologic oncology valid prognostic factors are necessary to define biologically similar subgroups for analysis of therapeutic efficacy. This study is the first published prospective study concerning prognostic significance of DNA ploidy and S‐phase fraction in cervical and endometrial cancer following enrichment of tumor cells by cytokeratin labelling. Epithelial cells were labeled by FITC‐conjugated cytokeratin antibody (CK 5, 6, 8, and CK 17) prior to flow cytometric cell cycle analysis in 91 specimens of cervical cancer and 73 samples of endometrial cancer. In cervical cancer neither DNA‐ploidy nor S‐phase fraction were relevant prognostic parameters. But CV of the G0G1‐peak showed prognostic relevance in cervical cancer cells, even in multivariate analysis. This interesting observation, however, seems to have no therapeutic consequence due to the small discrimination capacity of CV. In endometrial carcinoma, gross DNA‐aneuploidy (DNA‐index > 1.3) and a high percentage of proliferating cells (>75th percentile) were univariate and multivariate highly significant prognostic factors for recurrence‐free survival. Especially DNA‐aneuploidy (DI>1.3) is one of the most important independent molecular biological prognostic factors. While diagnostic curettage we could identify risk patients even preoperatively by determination of the prognostic factors like histologic tumor type, grading, cervical involvement and DNA‐ploidy. Thereby these patients could be treated primarily in an oncologic center. In conclusion, our investigations showed that the determination of DNA‐ploidy should be done in endometrial carcinoma. In cervical cancer no clinical significance for determination of DNA‐parameters was found.

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DNA-flow cytometry of squamous cell carcinomas from thehuman uterine cervix: The identification of prognostically different subgroups

Cited by 38 publications

References 18 publications

Ploidy, proliferative activity and prognosis. DNA flow cytometry of solid tumors

Ploidy, proliferative activity and prognosis. DNA flow cytometry of solid tumors

DNA flow cytometry in stage IB and II cervical carcinoma

Evaluation of Prognostic Factors Following Flow‐Cytometric DNA Analysis after Cytokeratin Labelling: II. Cervical and Endometrial Cancer

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