DNA fingerprinting of tilapia, Oreochromis niloticus, and its application to aquaculture genetics

Harris, Andrew; Bieger, Sophie; Doyle, Roger W.; Wright, Jonathan

doi:10.1016/0044-8486(91)90017-2

Cited by 73 publications

(32 citation statements)

References 21 publications

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“…3) Level of allele variation of C. macrocephalus microsatel lite loci (average 16.3 allele per locus) is quite high com pared to 7.7 alleles per locus (ranngge 5-14) reported for 7 microsatellite loci of C. gariepinus.24) However, the level of allele variation for Cga loci is possibly underestimated due to small sample size (37 individuals). For a riverine spe cies, Thai silver barb which is also native to Thailand, four Pgon loci were identified with average of 13.8 alleles per lo cus.2) High microsatellite allele variation was reported in numbers of fishes such as whiting (14)(15)(16)(17)(18)(19)(20)(21)(22)(23) The same individuals of the three populations used in this study, Prachinburi, Chiangrai and Pattani, were previ ously used for isozyme analyses.13) Heterozygosity based on data of 19 loci showed values of less than those of microsatellite loci by 10, 7 and 14 folds respective to Prachinburi, Chiangrai and Pattani. Because of the high variability detected by these microsatellite loci, we would suggest that they to be used for genetic characterization of domesticated stocks of C. macrocephalus which were previously reported to have low genetic variation (heter ozygosity=0.0) as revealed by isozyme studies.…”

Section: Gene Freauencies and Genetic Variabilitymentioning

confidence: 99%

Isolation and Characterization of Microsatellite Loci of <i>Clarias macrocephalus</i> and Their Application to Genetic Diversity Study

et al. 1999

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Four microsatellite loci were identified for Clarias macrocephalus and the forward and reverse primers for each locus were designed according to their unique flanking regions. One locus, Cma-1 *, was monomorphic, three loci, Cma-2*, Cma-3 * and Cma-4*, were polymorphic having 5, 14 and 30 al leles per locus respectively. The polymorphic microsatellite loci were studied in four natural populations of C. macrocephalus collected from different locations in Thailand. Mean number of alleles per locus were 8.0, 8.7, 6.0 and 10.0, respective to populations from Pattani, Pattalung, Chiangrai and Prachin buri. Mean heterozygosities were 0.744, 0.765, 0.718 and 0.810 respectively. Nei's genetic distance showed the closest relationship between Pattalung and Prachinburi (0.230) and the largest genetic dis tance between Pattalung and Chiangrai (0.535). However, the genetic distances obtained in this study were not accorded with geographic distance.

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Section: Gene Freauencies and Genetic Variabilitymentioning

confidence: 99%

Isolation and Characterization of Microsatellite Loci of <i>Clarias macrocephalus</i> and Their Application to Genetic Diversity Study

et al. 1999

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“…As such, these markers are widely used in population and conservation genetic studies as well as for the reconstruction of pedigrees and the estimation of relationships between individuals (Harris et al 1991, Goldstein & SchlÖtterer 1999.…”

Section: Introductionmentioning

confidence: 99%

Additional polymorphic microsatellite loci for detailed population genetic studies of Lethrus apterus (Coleoptera: Geotrupidae)

Rácz

Bereczki

Kosztolányi

et al. 2017

Acta Zool Acad Sci H

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In this study we attempted to isolate 13 additional microsatellites from the beetle Lethrus apterus and then to use them to screen for polymorphisms in 35 specimens collected from the northern Hungarian mountains. The newly developed markers presented in this paper are polymorphic, including between two and seven detected alleles per locus. The observed and expected heterozygosity per locus ranged between 0.057 and 0.686 and between 0.056 and 0.756, respectively, while two loci showed significant deviations from Hardy-Weinberg equilibrium. These deviations can be explained by the presence of null alleles. All loci cross-amplified in at least two out of four congeneric Lethrus species (L. bituberculatus, L. scoparius, L. strymonensis and L. perun). The polymorphic microsatellite markers we present for L. apterus will be valuable for future population genetic studies.

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“…Several DNA techniques for evaluating genetic variability in fish species are available and are widely used (Harris et al, 1991;Mjolnerod et al, 1997;Coughlan et al, 1998;Wasko and Galetti, 2002;Barman et al, 2003;Jayasankar, 2004;Matoso et al, 2004). One such technique is the Random amplified polymorphic DNA (RAPD) which was first introduced by Williams et al, (1990).…”

Section: Introductionmentioning

confidence: 99%

Genetic relationship between five fish species of genus scarus using rapd assay

Almaaty

El-Regal

Marie

et al. 2015

Egypt. J. of Aquatic Biolo. and Fish.

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Scaridae fish species including Scarus psittacus, S. frenatus, S. genazonatus, S. fuscopurpureus and S. niger, were collected from Red Sea in Egypt. The DNA fingerprints of these species were identified using RAPD-PCR technique with eight decamer primers. A total of 93 bands were scored in all species using the selected primers, with the size range varying from 190 to 2500 bp. On average, each primer amplified 11.63 bands per species. Each species had both monomorphic and polymorphic DNA bands. The polymorphic bands represent 34.41% in all fish species. The highest similarity index (70%) was found between Scarus psittacus and S. frenatus, while the lowest one (51%) was between Scarus psittacus and S. niger, S. genazonatus and S. fuscopurpureus and S. genazonatus and S. niger. Therefore the phylogenetic relationships among the five studied scaridae species were clearly resolved in dendrogram, they were clustered into four clusters groups: the first one comprised, S. fuscopurpureus and S. niger, the second included S. psittacus, S. frenatus and S. genazonatus, the third included S. genazonatus and the fourth branch contained S. psittacus and S. frenatus. In conclusion, the obtained results exhibited that the five Scarus species are distinctive ones. In addation, the RAPD patterns are suitable tools to differentiate between fish species.

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DNA fingerprinting of tilapia, Oreochromis niloticus, and its application to aquaculture genetics

Cited by 73 publications

References 21 publications

Isolation and Characterization of Microsatellite Loci of <i>Clarias macrocephalus</i> and Their Application to Genetic Diversity Study

Isolation and Characterization of Microsatellite Loci of <i>Clarias macrocephalus</i> and Their Application to Genetic Diversity Study

Additional polymorphic microsatellite loci for detailed population genetic studies of Lethrus apterus (Coleoptera: Geotrupidae)

Genetic relationship between five fish species of genus scarus using rapd assay

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