DNA damage exerted by mixtures of commercial formulations of glyphosate and imazethapyr herbicides in Rhinella arenarum (Anura, Bufonidae) tadpoles

Carvalho, Wanessa Fernandes; Arcaute, Celeste Ruiz de; Iglesias, Juan Manuel Pérez; Laborde, Milagros; Soloneski, Sonia; Larramendy, Marcelo L.

doi:10.1007/s10646-019-02029-x

Cited by 27 publications

(13 citation statements)

References 81 publications

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“…Thus, our findings represent the first experimental evidence obtained in a fish species, as C. decemmaculatus is, as the results of an in vivo exposure to these combinations when the primary DNA damage was employed as genotoxic end-point. However, a similar synergistic pattern has been previously described for the mixture of GLY-based Credit® plus dicamba-based herbicide Banvel® (Soloneski et al 2016) or by the former when combined with the herbicide imazethapyr-based formulation Pivot® H on R. arenarum tadpoles (Carvalho et al 2019) ) when applied in combination using specimens of the same fish species employed as an experimental matrix in our current study or by the combination. Likewise, when tadpoles of R. arenarum were exposed to a mixture of GLY-based Glifosato Atanor® or Glifoglex® plus cypermethrinbased insecticide Xiper® or Glextrin®, a lethal synergistic pattern was also achieved (Brodeur et al 2014).…”

Section: Discussionsupporting

confidence: 83%

Genotoxicity of mixtures of glyphosate with 2,4-dichlorophenoxyacetic acid chemical forms towards Cnesterodon decemmaculatus (Pisces, Poeciliidae)

Carvalho

Arcaute

Torres

et al. 2019

Environ Sci Pollut Res

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Acute genotoxicity of commercial glyphosate (GLY) (Credit®)-, 2,4-D-acid (2,4-D) (Dedalo Elite)-, 2,4-D-amine (2,4-D DMA) (Weedar Full®)-and 2,4-D-ester (2,4-D BE) (Herbifen Super®)-based herbicide formulations alone and their combinations were analysed in Cnesterodon decemmaculatus. Mortality was evaluated as a lethal end-point and the single cell gel electrophoresis (SCGE) bioassay was used as a sublethal end-point. LC50 96h values for Dedalo Elite was 0.46 mg/L and Herbifen Super® was 2.67 mg/L based on 2,4-D and 2,4-D BE, respectively. Results reveal a higher toxicity exerted on C. decemmaculatus after exposure to 2,4-D-rather than 2,4-D BE-based herbicide formulations. Overall, results demonstrated an enhancement in the genetic damage index committed to an enhancement of damaged erythrocytes of C. decemmaculatus when exposed to Credit®, Dedalo Elite, Weedar Full® and Herbifen Super® at 5% and 10% of LC50 96h values alone as well as in their combinations. Overall, the combination of GLY plus 2,4-D or GLY plus 2,4-D DMA showed a synergistic pattern whereas the combination of GLY plus 2,4-D BE was antagonic. Furthermore, this research is pioneer in the assessment of lethality and genotoxicity induced by 2,4-D-, 2,4-D DMA-and 2,4-D BE-based formulations when combined with GLY-based formulated herbicides in fish after they are acutely exposed.

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Section: Discussionsupporting

confidence: 83%

Genotoxicity of mixtures of glyphosate with 2,4-dichlorophenoxyacetic acid chemical forms towards Cnesterodon decemmaculatus (Pisces, Poeciliidae)

Carvalho

Arcaute

Torres

et al. 2019

Environ Sci Pollut Res

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“…In all surveys before herbicide application, more than 150 individuals of R. marina (see and approximately 100 individuals of S. ruber (L. Ferrante, personal observation) were recorded in areas of temporary puddles and artificial ponds (area 1,2 and 3 in Figure 1). The absence of these species in more than 250 m from the area of herbicide application (area 3 in Figure 1) is consistent with the results of a controlled study by Carvalho et al (2019), ACTA AMAZONICA which demonstrated the lethality of glyphosate on tadpoles of Rhinella arenarum. It is also consistent with the findings of , who documented the local extinction of Scinax caldarum in over 85% of its original range in the Atlantic Forest, likely caused by herbicide and pesticide use.…”

supporting

confidence: 88%

Evidence of mutagenic and lethal effects of herbicides on Amazonian frogs

Ferrante

Fearnside

2020

Acta Amaz.

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Amphibians are excellent bioindicators because they are sensitive to chemical pollution and can indicate ecosystem changes due to the presence of or exposure to chemical compounds. Here we report evidence of the impact of herbicides, including glyphosate, on amphibians in a locality in the central Brazilian Amazon and compare it with data from other biomes in Brazil. We observed malformations in three species of Leptodactylus and local extinctions of Scinax ruber and Rhinella marina from reproductive sites close to an area where herbicides had been applied. The observations in the Amazon are similar to reports from Brazil’s Atlantic forest on morphological anomalies and mortality in amphibians exposed to herbicides. We warn of the threat of expanding crops for the production of biofuels in the Amazon due to their cultivation being associated with agrochemicals, including glyphosate, posing a threat to the biodiversity of the Amazon biome.

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“…For instance, it has been found to cause oxidative stress and endocrine disruption in rats (Yang et al 2018) and developmental toxicity associated to thyroid dysfunction in zebrafish (Li et al 2020). Finally, imazethapyr has also been reported by causing metabolic alterations in Cyprinus carpio (Moraes et al 2011), genotoxicity in tadpoles of Rhinella arenarum and Hypsiboas pulchellus species (Carvalho et al 2019;Perez-Iglesias et al 2017;Perez-Iglesias et al 2015), and also in mammalian cells (Soloneski et al 2017); however, the toxicity of herbicide is poorly characterized and needs to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

Kuhn

Jacques

Teixeira

et al. 2021

Environ Sci Pollut Res

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Uruguay River is the most important river in western Rio Grande do Sul, separating Brazil from Argentina and Uruguay. However, its pollution is of great concern due to agricultural activities in the region and the extensive use of pesticides. In a long term, this practice leads to environmental pollution, especially to the aquatic system. The objective of this study was to analyze the physicochemical characteristics, metals and pesticides levels in water samples obtained before and after the planting and pesticides' application season from three sites: Uruguay River and two minor affluents, Mezomo Dam and Salso Stream. For biomonitoring, the free-living nematode Caenorhabditis elegans was used, which were exposed for 24 h. We did not find any significant alteration in physicochemical parameters. In the pre-and post-pesticides' samples we observed a residual presence of three pesticides (tebuconazole, imazethapyr, and clomazone) and metals which levels were above the recommended (As, Hg, Fe, and Mn). Exposure to both pre-and post-pesticides' samples impaired C. elegans reproduction and post-pesticides samples reduced worms' survival rate and lifespan. PCA analysis indicated that the presence of metals and pesticides are important variables that impacted C. elegans biological endpoints. Our data demonstrates that Uruguay River and two affluents are contaminated independent whether before or after pesticides' application season. In addition, it reinforces the usefulness of biological indicators, since simple physicochemical analyses are not sufficient to attest water quality and ecological safety.

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DNA damage exerted by mixtures of commercial formulations of glyphosate and imazethapyr herbicides in Rhinella arenarum (Anura, Bufonidae) tadpoles

Cited by 27 publications

References 81 publications

Genotoxicity of mixtures of glyphosate with 2,4-dichlorophenoxyacetic acid chemical forms towards Cnesterodon decemmaculatus (Pisces, Poeciliidae)

Genotoxicity of mixtures of glyphosate with 2,4-dichlorophenoxyacetic acid chemical forms towards Cnesterodon decemmaculatus (Pisces, Poeciliidae)

Evidence of mutagenic and lethal effects of herbicides on Amazonian frogs

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

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