DNA damage and ovarian ultrastructural lesions induced by nickel oxide nano-particles in Blaps polycresta (Coleoptera: Tenebrionidae)

Kheirallah, Dalia A.; El-Samad, Lamia M.; Abdel‐Moneim, Ashraf M.

doi:10.1016/j.scitotenv.2020.141743

Cited by 29 publications

(11 citation statements)

References 75 publications

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“…The LD 50 was calculated in the current study from the mortality test ( S2 Fig), and the 0.01 mg/g sublethal dose was chosen to detect the early apoptotic damage in the ovarian tissue of the tested beetles. Our earlier experiment on the ground beetle Blaps polycresta [34] validated the efficacy of the injection method of NPs in insects. The study also indicated that the early signs of cell injuries could be distinguished at the sublethal dose of the tested NPs.…”

Section: Plos Onementioning

confidence: 66%

“…Consequently, understanding their behaviour in biological markers is important. Therefore, terrestrial insects, especially beetles, are acceptable biomarkers that can assess nanotoxicity [34]. Any disturbance in the biological processes in exposed insects may be considered clues for nanotoxicity that might be expected in other living beings.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, some interstitial cells were degenerated. Kheirallah et al [34] observed such anomalies in the ovarian cells of beetles injected with a sublethal dose of NiO-NPs. The follicular epithelial cells (FECs) exhibited nuclear and cytoplasmic malformations.…”

Section: Plos Onementioning

confidence: 99%

“…Au-NPs [34] were bought from Nanotech Egypt for Photo-Electronics in the liquid form. The characterisation of Au-NPs was illustrated by the TEM (JEOL, JEM-1400 plus Electron Microscope).…”

Section: Characterisation Of Au-npsmentioning

confidence: 99%

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Relative expression of microRNAs, apoptosis, and ultrastructure anomalies induced by gold nanoparticles in Trachyderma hispida (Coleoptera: Tenebrionidae)

et al. 2020

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The extensive use of nanomaterials generates toxic effects on non-target species and the ecosystem. Although gold nanoparticles (Au-NPs) are generally expected to be safe, the recent study contains conflicting data regarding their cytotoxicity in the darkling beetles Trachyderma hispida. The study postulated cellular perturbation in the ovarian tissue of the beetles induced by a sublethal dose of Au-NPs (0.01 mg/g). When compared with the controls, a significant inhibition in the activities of the antioxidant enzymes selenium-dependent (GPOX) and selenium-independent (GSTP) glutathione peroxidases (GPx) was observed in the treated beetles. The study proposed microRNAs (miRNA-282 and miRNA-989) as genotoxic markers for the first time, reporting a significant suppression in their transcriptional levels in the treated beetles. Furthermore, TUNEL (Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) and flow cytometry assays (annexin V-Fitc) indicated a significant increase in ovarian cell apoptosis in the treated beetles. Additionally, an ultrastructure examination revealed pathological changes in the ovarian cells of the treated beetles. The resulting anomalies in the present study may interrupt the fecundity of the beetles and lead to the future suppression of beetle populations.

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Section: Plos Onementioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

“…Au-NPs [34] were bought from Nanotech Egypt for Photo-Electronics in the liquid form. The characterisation of Au-NPs was illustrated by the TEM (JEOL, JEM-1400 plus Electron Microscope).…”

Section: Characterisation Of Au-npsmentioning

confidence: 99%

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Relative expression of microRNAs, apoptosis, and ultrastructure anomalies induced by gold nanoparticles in Trachyderma hispida (Coleoptera: Tenebrionidae)

et al. 2020

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“…No previous data demonstrated an effect of nickel oxide nanoparticles (NiO-NPs) in the testes of ground beetles. However, NiO-NPs induced genotoxicity and ultrastructure alterations in the ovarian tissues of B. polycresta (Kheirallah et al, 2020).…”

Section: Introductionmentioning

confidence: 94%

Nickel oxide nanoparticles induce genotoxicity and cellular alterations in the ground beetleBlaps polycresta(Coleoptera: Tenebrionidae)

et al. 2021

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Nickel nanoparticles (Ni-NPs) have advantageous applications in the industry; however, little is known of their adverse effects on biological tissues. In the present study, the ground beetle Blaps polycresta was employed as a sensitive indicator for nickel oxide nanoparticles (NiO-NPs) toxicity. Adult male beetles were injected with six dose levels of NiO-NPs (0.01, 0.02, 0.03, 0.04, 0.05, and 0.06 mg/g body weight). Mortality was reported daily over 30 days under laboratory conditions to establish an LD50. Nickel was detected in the testicular tissues of the beetles using X-ray analysis and transmission electronic microscopy. Beetles treated with the sublethal dose of 0.02 mg/g were selected to observe molecular, cellular, and subcellular changes. Gene transcripts of HSP70, HSP90, and MT1 were found to be increased >2.5-, 1.5-, and 2-fold, respectively, in the treated group compared with the controls. Decreased gene expression of AcPC01, AcPC02, and AcPC04 (≤1.5-, ≤2-, and < 2.5-fold, respectively, vs. controls) also were reported in the treated group. Under light microscopy, various structural changes were observed in the testicular tissues of the treated beetles. Ultrastructure observations using scanning and transmission electron microscopy showed severe damage to the subcellular organelles as well as deformities of the heads and flagella of the spermatozoa. Therefore, the present study postulated the impact of NiO-NPs in an ecological model.

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Smart Nanocarrier-Based Cancer Therapeutics

Awan

Naeem

Saeed

et al. 2023

Cancer Treatment and Research

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DNA damage and ovarian ultrastructural lesions induced by nickel oxide nano-particles in Blaps polycresta (Coleoptera: Tenebrionidae)

Cited by 29 publications

References 75 publications

Relative expression of microRNAs, apoptosis, and ultrastructure anomalies induced by gold nanoparticles in Trachyderma hispida (Coleoptera: Tenebrionidae)

Relative expression of microRNAs, apoptosis, and ultrastructure anomalies induced by gold nanoparticles in Trachyderma hispida (Coleoptera: Tenebrionidae)

Nickel oxide nanoparticles induce genotoxicity and cellular alterations in the ground beetleBlaps polycresta(Coleoptera: Tenebrionidae)

Smart Nanocarrier-Based Cancer Therapeutics

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