DNA Content of Human Squamous Cell Carcinoma Cell Lines: Analysis by Flow Cytometry and Chromosome Enumeration

HPV‐negative and HPV‐positive HNSCC cell lines show similar numerical but different structural chromosomal aberrations

“…We also observed that for both HPV‐negative as well as HPV‐positive cell lines, there was an excellent and identical correlation between the DNA index and the respective mean number of chromosomes (Figure D) as previously already observed by Roa et al This finding demonstrates that for both entities DNA aneuploidy is primarily caused by an amplification of whole chromosomes and less due to an amplification of certain genomic regions, which is in line with the general concept of tumor aneuploidy …”

“…In addition, an increased number of chromosomes with mean values of 66 and 68 were determined in both groups (Figures and ). For DNA ploidy, similar data were measured by others also showing no difference between HPV‐negative and HPV‐positive cell lines. Quite similar values for the mean numbers of chromosomes were previously found for other HNSCC cell lines …”

DNA Gains and Losses of Chromosome in Laryngeal Squamous Cell Carcinoma Using Comparative Genomic Hybridization

“…Polyploidies were identified in 3 cases using the CGH technique. Polyploidy was also reported cytogenetically in short term cultures from biopsy specimens,24 cell lines25 and in tumor cells by flow cytometry 26. Aneuploidy, another sign of genomic instability in tumors, was observed in some of the patients in this study along with other chromosome changes, in the form of whole chromosome gains (Table 1).…”

“…9p23-pter and Xq27-qter amplifications have not previously been reported to occur in LSCCs, while amplification of a region including 5p12.2-pter was reported in a CGH study in 2 out of 38 cases of primary LSCCs 9. The 5p15.2-pter region lies within 5p14-pter, amplification of which has previously been reported in 2 out of 23 having primary larynx tumors with metastases to adjacent lymph nodes 25. However, two of our three cases with a polyploidy profile had metastases, whereas two with 5p15.2-pter had no distant metastases.…”

Characterization of newly established oral cancer cell lines derived from six squamous cell carcinoma and two mucoepidermoid carcinoma cells

“…However, the establishment of an oral cancer cell line is especially difficult from the primary site, and only a few successful attempts have been reported (Easty et al, 1981;Hu et al, 1984;Roa et al, 1985;Sacks et al, 1988;Tatke et al, 1995;Ji et al, 2001). Most previously established cell lines have been reported from lymph node metastatic foci, or transplanted tumors in nude mice.…”