DNA Binding to Hydroxyapatite: A Potential Mechanism for Preservation of Microbial DNA

Brundin, Malin; Figdor, David; Sundqvist, Göran; Sjögren, U.

doi:10.1016/j.joen.2012.09.013

Cited by 71 publications

(59 citation statements)

References 40 publications

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“…[10][11][12][13] Thus, DNA-HAp NPs complexes were found to cross cell membranes, integrating into the cell genome. In a very recent study, Brundin et al 14 showed a specific binding affinity of HAp for DNA. These same authors found that HAp-bound DNA is more resistant to decay and less susceptible to degradation by serum and nucleases, which may account for the long-term persistence of DNA in bone and tooth.…”

Section: Introductionmentioning

confidence: 98%

“…These same authors found that HAp-bound DNA is more resistant to decay and less susceptible to degradation by serum and nucleases, which may account for the long-term persistence of DNA in bone and tooth. 14 The direct encounter between DNA and HAp in biological systems suggests that investigation of the interaction between these two materials and particularly, of the formation of biominerals through the nucleation of HAp at a DNA template † These authors contributed equally to this work. a would be very useful for the development of novel strategies for gene therapy.…”

Section: Introductionmentioning

confidence: 99%

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Mineralization of DNA into nanoparticles of hydroxyapatite

et al. 2014

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Mineralization of DNA into nanoparticles of hydroxyapatite

et al. 2014

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“…The long-term persistence of DNA in skeletons has been associated with the fact that it can bind to hydroxyapatite, the main mineral component of bone tissue, which makes it more resistant to decay and less susceptible to degradation by enzymes [9]. In addition, DNA has been shown to bind to collagen forming a DNA-collagen complex [10], and can be extracted from archaeological bone powder in both the hydroxyapatite and collagen fractions [11].…”

Section: Introductionmentioning

confidence: 99%

DNA and bone structure preservation in medieval human skeletons

Coulson-Thomas

Norton

Coulson‐Thomas

et al. 2015

Forensic Science International

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Nader, DNA and bone structure preservation in medieval human skeletons, Forensic Science International (2015), http://dx.doi.org/10.1016/j.forsciint. 2015.04.005 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A c c e p t e d M a n u s c r i p t AbstractMorphological and ultrastructural data from archaeological human bones are scarce, particularly data that have been correlated with information on the preservation of molecules such as DNA. Here we examine the bone structure of macroscopically wellpreserved medieval human skeletons by transmission electron microscopy and immunohistochemistry, and the quantity and quality of DNA extracted from these skeletons. DNA technology has been increasingly used for analyzing physical evidence in archaeological forensics; however, the isolation of ancient DNA is difficult since it is highly degraded, extraction yields are low and the co-extraction of PCR inhibitors is a problem. We adapted and optimized a method that is frequently used for isolating DNA from modern samples, Chelex® 100 (Bio-Rad) extraction, for isolating DNA from archaeological human bones and teeth. The isolated DNA was analysed by real-time PCR using primers targeting the sex determining region on the Y chromosome (SRY) and STR typing using the AmpFlSTR® Identifiler PCR Amplification kit. Our results clearly show the preservation of bone matrix in medieval bones and the presence of intact osteocytes with well preserved encapsulated nuclei. In addition, we show how effective Chelex® 100 is for isolating ancient DNA from archaeological bones and teeth. This optimized method is suitable for STR typing using kits aimed specifically at degraded and difficult DNA templates since amplicons of up to 250 bp were successfully amplified.

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“…Les analyses d'ADN ancien sont le plus fréquemment réalisées à partir de tissus osseux et dentaires qui possèdent un microenvironnement favorable à la conservation de fragments d'ADN grâce à leur adsorption sur une matrice minérale, l'hydroxyapatite [1]. Mais des analyses peuvent également être réalisées à partir de tissus mous momifiés ou de restes pileux.…”

Section: Matériel Et Techniques D'études Matérielunclassified

“…En effet, cet ADN est 1 Grâce à l'analyse de l'ADN présent dans les tissus humains anciens et au développement de marqueurs génétiques qui permettent d'explorer de courtes séquences de cette molécule, il est actuellement possible d'étudier les populations du passé, leur structure, leur migration, ainsi que la dynamique de peuplement de certaines régions du monde. En effet, l'étude du profil génétique de sujets inhumés dans des nécropoles permet de déterminer leurs liens de parenté avec d'autres sujets, d'estimer leur origine biogéographique ainsi que les voies de migration qu'ils ont pu emprunter il y a des centaines ou milliers d'années, de comparer leurs caractéristiques géné-tiques avec celles des populations actuelles, ou encore d'établir certains de leurs traits physiques.…”

Section: Adn Et Marqueurs Moléculaires Utilisésunclassified