DNA Barcoding Medicinal Plant Species from Indonesia

Cahyaningsih, Ria; Compton, Lindsey; Rahayu, Rahayu; Brehm, Joana Magos; Maxted, Nigel

doi:10.3390/plants11101375

Cited by 23 publications

(16 citation statements)

References 48 publications

(72 reference statements)

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“…Therefore, in addition to improving the plant identification database, the DNA barcoding techniques has been well-employed to identify the biodiversity of medicinal plants in Indonesia. A group of researcher examined four distinct DNA barcoding regions-rbcL, ITS2, trnL, and matK-to identify 61 species of Indonesian medicinal plants, including two IUCN vulnerable (VU) species: the Aquilaria hirta and the Etlingera solaris (Cahyaningsih et al, 2022). Importantly, the results of this study also support the idea that DNA barcoding can be used to distinguish between pure extract of medicinal herbs compared to fake substitutes (Mishra et al, 2016).…”

Section: Indonesian Dna Barcode Research Trendsupporting

confidence: 52%

“…In Indonesia, the use of DNA barcoding technique in the identification of flowering and medicinal plants has been increasing in recent years (Amandita et al, 2019;Cahyaningsih et al, 2022). Tropical rainforests of Sumatra in Indonesia provide an abundance of vegetation and wildlife (Basyuni et al, 2019;Breidenbach et al, 2018;Rambey et al, 2021).…”

Section: Indonesian Dna Barcode Research Trendmentioning

confidence: 99%

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A Bibliometric Analysis of Indonesia Biodiversity Identification through DNA Barcoding Research from 2004-2021

Priyono¹,

Sofyantoro²,

Putri³

et al. 2023

NLSC

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Indonesia is well-known for having a vast and rich endowment of unique and genetically diverse biodiversity resources. Currently, initiatives are taking place around the world to generate DNA barcode libraries to make these data available to better understand biodiversity. The objectives of this study are to document DNA barcode research trends and detect the extent to which its application has evolved in Indonesia. The analysis was investigated using a compilation of 446 published papers, obtained from Harzing's Publish or Perish 8. The number of DNA barcode publication records has increased by a geometric average of 15.4/year. The number of studies involving molecular identification (30.1%), species and genetic diversity (10%), and evolutionary or phylogenetic studies (10%) appears to have driven much of the publication activity. The top three taxa studied include fishes (32.7%), plants (24.8%), and invertebrates (12.5%; except insects). We discovered that using a single molecular marker is still dominant (62.8%). We conclude that the practices of DNA barcoding data are likely to become a valuable resource in many sectors and focuses. However, the number of Indonesian DNA barcode records in public databases is relatively lower than in other mega biodiversity countries. The establishment of DNA barcoding initiatives and a national DNA barcode reference library in Indonesia would promote DNA barcoding applications to help conserve Indonesia biodiversity. Keywords: Biodiversity, DNA barcode, Indonesia, Research trends

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Section: Indonesian Dna Barcode Research Trendsupporting

confidence: 52%

Section: Indonesian Dna Barcode Research Trendmentioning

confidence: 99%

A Bibliometric Analysis of Indonesia Biodiversity Identification through DNA Barcoding Research from 2004-2021

Priyono¹,

Sofyantoro²,

Putri³

et al. 2023

NLSC

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“…Previous studies have shown that the identification of DNA barcoding target species does not possess an optimal or flawless region. Despite this, they suggest the utilization of matK as the preferred barcode for identifying Indonesian medicinal plants [24,25]. Nevertheless, the use of DNA barcoding has been focused on a single region for most therapeutic plants.…”

Section: Discussionmentioning

confidence: 99%

DNA barcoding application for potential medicinal plants on Ziziphus species from Aketajawe-Lolobata National Park, North Moluccas

Arifiani,

Sulistyaningsih,

Martiansyah

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Aketajawe-Lolobata National Park is a protected national park located in the province of North Moluccas, Indonesia. The national park has been known to be rich in biodiversity with the existence of many potential medicinal plants such as Ziziphus. DNA barcoding can be a useful tool for identifying and characterizing these plants for potential medical applications. Research and exploration have been conducted in Aketajawe-Lolobata National Park in November and December 2022. This study aimed to analyze how the DNA barcoding regions aid identification for potential medicinal plants. The genomic DNA were amplified by matK primers, and the sequences showed high similarity rate with their homologs, then the phylogenetic tree was constructed using the Neighbor-Joining method to assess their cross-species relationship. The phylogenetic study showed that the species that have been identified based on morphological characters, i.e., Ziziphus angustifolia and Z. mauritiana were clustered with Z. angustifolia and Z. mauritiana retrieved from GenBank. However, the unidentified species are closely related with Z. calophylla obtained from GenBank but with low bootstrap support. All the results indicate that DNA barcoding approach could be successfully used for reliable identification of medicinal plants and matK gene is a good candidate for this approach.

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“…Therefore, high quality of DNA extracted was a crucial step in any DNA molecular techniques. This DNA barcoding approach served as a new detection tool for identifying adulterated herbal medicinal products [4,16,18,25]. However, the DNA extraction from complex herbal products is challenging due to mixture of several of plants species that containing different polyphenols, alkaloids, and flavonoids [15] or mixed with other substance (talc/pharmaceutical excipients in their formulation, pigment, stabilizer, impurities, admixtures).…”

Section: Introductionmentioning

confidence: 99%

EVALUATION OF DNA QUALITY FROM MODIFIED DNA EXTRACTION AND AMPLIFICATION OF ITS2 FROM Eurycoma longifolia CAPSULE HERBAL PRODUCTS

Izham

Saidon²,

Samad³

et al. 2022

Jurnal Teknologi

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Demand for herbal products has increased because of their purported health benefits and economic value. However, they are susceptible to adulteration, making accurate identification of herbal origin essential, especially for quality control. In recent decades, DNA-based methods have played a crucial role in the development of authentication tools that required good quality of DNA. The manufacturing process of herbal products involved heating, grinding or other mechanical procedures, and addition of excipients/additives caused DNA to degrade which in turn influenced DNA quality. In this study, nine different conventional methods with some modifications were evaluated to determine the best technique producing good quality DNA from capsule herbal products. Assessment was conducted using spectrophotometric measurements and agarose gel electrophoresis. To determine the quality of gDNA, amplification of ITS2 amplicon was performed by PCR. The DNA extraction finding showed that DNA quality from each method resulted in a different DNA purity and yield, hence the ITS2 amplification. Each of the modified DNA extraction methods performed has its own strengths and limitations when it comes to obtaining high quality gDNA. In addition, the study demonstrated the success of ITS2 amplification with the modified DNA extraction methods used.

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DNA Barcoding Medicinal Plant Species from Indonesia

Cited by 23 publications

References 48 publications

A Bibliometric Analysis of Indonesia Biodiversity Identification through DNA Barcoding Research from 2004-2021

A Bibliometric Analysis of Indonesia Biodiversity Identification through DNA Barcoding Research from 2004-2021

DNA barcoding application for potential medicinal plants on Ziziphus species from Aketajawe-Lolobata National Park, North Moluccas

EVALUATION OF DNA QUALITY FROM MODIFIED DNA EXTRACTION AND AMPLIFICATION OF ITS2 FROM Eurycoma longifolia CAPSULE HERBAL PRODUCTS

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