DNA Barcodes Can Distinguish Species of Indian Mosquitoes (Diptera: Culicidae)

Kumar, N. Pradeep; Rajavel, A. R.; Natarajan, R.; Jambulingam, P.

doi:10.1093/jmedent/41.5.01

Cited by 232 publications

(126 citation statements)

References 19 publications

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“…Low intra-specific sequence divergence (mean genetic distance = 0.000-0.018) of ITS2, COI, and COII of the four forms provides good supportive evidence. The maximum intra-species K2P values based on COI barcoding sequences obtained from this study were similar to that reported for Anopheles pallidus (0.0184) [33]. Kumar et al [33] denoted that the K2P values were > 0.02 between different species for Culicidae.…”

Section: Discussionsupporting

confidence: 88%

“…The maximum intra-species K2P values based on COI barcoding sequences obtained from this study were similar to that reported for Anopheles pallidus (0.0184) [33]. Kumar et al [33] denoted that the K2P values were > 0.02 between different species for Culicidae. Our findings are in agreement with the results of cross-matings among karyotypic forms of other anophelines previously reported by several investigators, i.e., Anopheles vagus [34], A. pullus (= Anopheles yatsushiroensis) [35], A. sinensis [36][37][38][39], Anopheles aconitus [25], Anopheles barbirostris A1 and A2 [11,29], Anopheles campestris-like [30], Anopheles peditaeniatus [31,32], and Anopheles paraliae [40].…”

Section: Discussionsupporting

confidence: 88%

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Cytogenetic, cross-mating and molecular evidence of four cytological races of Anopheles crawfordi (Diptera: Culicidae) in Thailand and Cambodia

Saeung

Baimai

Thongsahuan

et al. 2014

Comptes Rendus. Biologies

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 88%

Cytogenetic, cross-mating and molecular evidence of four cytological races of Anopheles crawfordi (Diptera: Culicidae) in Thailand and Cambodia

Saeung

Baimai

Thongsahuan

et al. 2014

Comptes Rendus. Biologies

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“…Several workers have used COI as the only marker in mosquito species recognition and in investigating their molecular evolution [3–9]. However, some studies have shown that mosquito identification through COI alone is not always sufficient to make precise conclusions and the need for genetic examination using analysis of faster evolving regions, such as ITS2, has been emphasized [8].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Anopheline (Diptera: Culicidae) mosquitoes from eight geographical locations of Sri Lanka

Weeraratne

Surendran

Reimer

et al. 2017

Malar J

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BackgroundGenus Anopheles is a major mosquito group of interest in Sri Lanka as it includes vectors of malaria and its members exist as species complexes. Taxonomy of the group is mainly based on morphological features, which are not conclusive and can be easily erased while handling the specimens. A combined effort, using morphology and DNA barcoding (using the markers cytochrome c oxidase subunit I (COI) gene and internal transcribed spacer 2 (ITS2) region, was made during the present study to recognize anophelines collected from eight districts of Sri Lanka for the first time.Methods Cytochrome c oxidase subunit I and ITS2 regions of morphologically identified anopheline mosquitoes from Sri Lanka were sequenced. These sequences together with GenBank sequences were used in phylogenetic tree construction and molecular characterization of mosquitoes.ResultsAccording to morphological identification, the field-collected adult mosquitoes belonged to 15 species, i.e., Anopheles aconitus, Anopheles annularis, Anopheles barbirostris, Anopheles culicifacies, Anopheles jamesii, Anopheles karwari, Anopheles maculatus, Anopheles nigerrimus, Anopheles pallidus, Anopheles peditaeniatus, Anopheles pseudojamesi, Anopheles subpictus, Anopheles tessellatus, Anopheles vagus, and Anopheles varuna. However, analysis of 123 COI sequences (445 bp) (16 clades supported by strong bootstrap value in the neighbour joining tree and inter-specific distances of >3%) showed that there are 16 distinct species. Identity of the morphologically identified species, except An. subpictus, was comparable with the DNA barcoding results. COI sequence analysis showed that morphologically identified An. subpictus is composed of two genetic entities: An. subpictus species A and species B (inter-specific K2P distance 0.128). All the four haplotypes of An. culicifacies discovered during the present study belonged to a single species. ITS2 sequences (542 bp) were obtained for all the species except for An. barbirostris, An. subpictus species B, An. tessellatus, and An. varuna. Each of these sequences was represented by a single species-specific haplotype.ConclusionsThe present study reflects the importance and feasibility of COI and ITS2 genetic markers in identifying anophelines and their sibling species, and the significance of integrated systematic approach in mosquito taxonomy. Wide distribution of malaria vectors in the country perhaps indicates the potential for re-emergence of malaria in the country.Electronic supplementary materialThe online version of this article (doi:10.1186/s12936-017-1876-y) contains supplementary material, which is available to authorized users.

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“…Kent et al 2004, Marrelli et al 2005, Michel et al 2005). However, a number of recent studies have shown that the standard COI barcode marker also serves effectively for species-level discrimination in surveys of Canadian (Cywinska et al 2006) and Indian (Kumar et al 2007) mosquitoes. Foley et al (2007) constructed a molecular phylogeny of the Australian Anopheles annulipes species complex based on four different loci, both nuclear and mitochondrial (COI, COII, ITS2, and EF-1α).…”

Section: Dipteramentioning

confidence: 99%