DNA aptamer as molecular tool for ZIKV NS1 protein detection

Morais, Liliane; Alves, Laís Nascimento; Argondizzo, Ana Paula Corrêa; Rocha, Henrique Francisco; Silva, Dı́lson; Valdez, E. C. N.; Filippis, Ana María Bispo de; Missailidis, Sotiris

doi:10.1063/1.5079162

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…They also obtained a second aptamer (APT10) with negligible cross-reactivity, but less affinity ( K d = 134 nM) [ 43 ]. In another study, Morais et al performed a DNA aptamer selection by immobilizing ZIKV NS1 on ELISA plates [ 44 ]. Seven selection cycles were used, including a counterselection step for YFV NS1 in the last cycle.…”

Section: Resultsmentioning

confidence: 99%

DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection

Almeida¹,

Sousa²,

Santos³

et al. 2022

Beilstein J. Nanotechnol.

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Zika virus (ZIKV) is a mosquito-borne virus that is phylogenetically close to other medically important flaviviruses with high global public health significance, such as dengue (DENV) and yellow fever (YFV) viruses. Correct diagnosis of a flavivirus infection can be challenging, particularly in world regions where more than one flavivirus co-circulates and YFV vaccination is mandatory. Acid nucleic aptamers are oligonucleotides that bind to a specific target molecule with high affinity and specificity. Because of their unique characteristics, aptamers are promising tools for biosensor development. Aptamers are usually obtained through a procedure called “systematic evolution of ligands by exponential enrichment” (SELEX). In this study, we select an aptamer (termed ZIKV60) by capillary electrophoresis SELEX (CE-SELEX) to the Zika virus non-structural protein 1 (NS1) and counterselection against the NS1 proteins of DENV (serotypes 1, 2, 3, and 4) and YFV. The ZIKV60 dissociation constant (Kd) is determined by enzyme-linked oligonucleotide assay (ELONA) and the aptamer specificity is evaluated by quantitative real-time polymerase chain reaction. ZIKV60 shows a high binding affinity to the ZIKV NS1 protein with a Kd value of 2.28 ± 0.28 nM. The aptamer presents high specificity for ZIKV NS1 compared to NS1 of DENV and YFV. Furthermore, graphene field-effect transistor devices functionalized with ZIKV60 exhibit an evident identification of NS1 protein diluted in human serum. These results point to the applicability of biosensors based on the ZIKV60 aptamer for the differential diagnosis of the Zika virus.

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Section: Resultsmentioning

confidence: 99%