DNA and its synthesis in individual tumor cells from human upper respiratory tract squamous cell carcinomas

Le, Holm; Jakobsson, P-J; Killander, D.; Silfverswärd, Claes; Wersäll, Jan

doi:10.1288/00005537-198007000-00017

Cited by 29 publications

(8 citation statements)

References 18 publications

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“…DNA measurements of squamous cell carcinomas of the head and neck region show similar DNA ploidy for tumours from various locations, and most tumours in the series reported by Holm et al, (1980) and Holm (1982) were DNA non-diploid. However, most tumours in the present series were DNA diploid (56%), possibly because small tumours tend to be DNA diploid (Kaplan et al, 1986;Tytor et al, 1987).…”

Section: Resultsmentioning

confidence: 65%

“…DNA content, proliferative activity (Sphase), and the occurrence of polyploid nuclei are more objective estimates and yield important complements to the clinical and pathological classification of many types of tumours (Atkin, 1976;Friedlander et al, 1984;Matsuura et al, 1986). Studies by Holm et al (1980) and Holm (1982) indicate that DNA measurements of oral cavity carcinomas may contain prognostic information.…”

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confidence: 99%

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DNA content, malignancy grading and prognosis in T1 and T2 oral cavity carcinomas

Tytor

Franzen²,

Olofsson³

et al. 1987

Br J Cancer

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Summary Microscopic malignancy grading using the 8-factor system proposed by Jakobsson et al. (1973), the 4-factor system set up by Glanz and Eichhorn (1985), and DNA cytofluorometry were applied to thirteen Ti and thirty-seven T2 squamous cell carcinomas of the oral cavity, 9 with and 41 without metastases. There was a significant correlation between the presence of lymph node metastases (Ni) and the malignancy scores (P<0.05) and tumour DNA ploidy (P<0.01, chi-square). The total number of patients with initial and late lymph node metastases correlated significantly with polyploid nuclei (P<0.05) and with malignancy scores (P<0.001), which also correlated with regional recurrences (P<0.01, chi-square). No remaining tumour after preoperative radiotherapy indicated less risk for local recurrence than if tumour persisted (P<0.01, chisquare).The cumulative survival (Kaplan-Meier) was worse for patients with nodal involvement (Nl) than for those without (NO) (P<0.01), and for patients with poorly differentiated tumours compared with moderately well differentiated (P<0.05) and to well differentiated (P<0.001). The prognosis was worse for patients with high malignancy scores than for those with low (P<0.001). DNA diploid tumours had a better prognosis than DNA non-diploid, but the difference was not significant.Despite the various modes of treatment available, patients with oral cavity carcinoma pose serious therapeutic problems which are reflected in the poor survival rates (Frazell et al., 1962). Factors influencing the prognosis are therefore sought after. The site and size of the primary tumour and the presence of metastases have been used as prognostic indicators (Lee et al., 1972;Krause et al., 1973;Fletcher, 1979). The presence of cervical lymph node metastases seems to be the most important predictor associated with approximately 50 per cent reduction of the 2-year determinate survival rate (Hibbert et al., 1983; Teichgraeber et al., 1973;Willen et al., 1975; Lund et al., 1975) have cavity carcinoma provides useful prognostic information but has certain limitations. The single method treatment of stage I cancer carries a much poorer prognosis than was previously thought, and the incidence of microscopic cervical metastases is high (Lee et al., 1972;Krause et al., 1973;Teichgraeber et al., 1984).The malignancy grading system based on 4 different morphological characteristics for the tumour cell population, and 4 characteristics for the tumour-host relationship, initially used in the analysis of laryngeal cancer (Jakobsson et al., 1973), has also been applied in oral cavity carcinomas.Studies on palatal, gingival, and lingual carcinomas Willen et al., 1975; Lund et al., 1975) have disclosed statistically significant differences in survival for patients with high and low malignancy scores. The somewhat modified malignancy grading used in lingual carcinomas by Holm et al. (1982) showed a correlation between the malignancy score on the one hand and T classification or the presence of lymph node metastases at th...

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Section: Resultsmentioning

confidence: 65%

mentioning

confidence: 99%

DNA content, malignancy grading and prognosis in T1 and T2 oral cavity carcinomas

Tytor

Franzen²,

Olofsson³

et al. 1987

Br J Cancer

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“…Extensive studies of other head and neck malignancies using flow cytometry have produced varied results [11, 12, 13, 14, 15]. Nonetheless, a strong correlation exists between nuclear DNA content and prognosis in upper respiratory tract carcinomas [16]. …”

Section: Discussionmentioning

confidence: 99%

Flow Cytometry and Squamous Cell Carcinoma of the Maxillary Sinus: A Possible Prognostic Indicator for Multimodality Intervention

Halvorson

Day²,

Christian³

et al. 1999

Oncology

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Malignant tumors of the paranasal sinuses constitute less than 1% of all malignancies. Unfortunately, few prognostic factors have been identified regarding the efficacy of interventional therapy. Patients with carcinoma of the maxillary sinus frequently present with an advanced stage of disease and multimodality therapy is often proposed. Although some tumors appear to be more sensitive to preoperative chemotherapy and radiation therapy, a method of predicting and evaluating tumor behavior has not been recognized. Introduced as a means of identifying cell populations with abnormalities in total DNA content, flow-cytometric DNA analysis provides a quick and reliable means of tumor characterization for many malignancies. Although nuclear DNA content has been implicated as a prognostic factor in an increasing number of tumor types, current data on the role of DNA content in head and neck carcinoma is conflicting and incomplete. To evaluate the role of flow-cytometric DNA analysis in predicting therapeutic alternatives, 22 patients with squamous cell carcinoma of the maxillary sinus were reviewed. Patient outcome and histopathologic grade were retrospectively compared with flow-cytometric evaluations of paraffin-embedded formalin-fixed tumor specimens. Four of sixteen tumors (25%) were found to be aneuploid and were also associated with an increased survival rate (p < 0.01). This initial data emphasizes the possible usefulness of DNA measurement for characterization of squamous cell carcinoma of the maxillary sinus and stresses the need for further evaluation.

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“…These subjects must be questioned objectively. Ganzer et al [16] reported an average labeling index which varied from 4.1 to 14.6% from tumor to tumor specimen Holm et al [17] also felt that irregular thymidine incorporation was secondary to poor penetration during the 1-hour incubation and recommended suspension techniques.…”

Section: Methodological Reasonsmentioning

confidence: 99%

Thymidine Labeling Index in Laryngeal Squamous Cell Carcinoma in Correlation with pTNM, Age, Histological Grade and Recurrence

Ünal

Bılır²,

Karatay

2002

ORL

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In this study, our aim was to investigate the prognostic features of the thymidine labeling index (TLI) in laryngeal squamous cell carcinoma (SCC). The TLI values in tumor tissues and adjacent healthy tissues were assessed in 40 patients who had a pathological diagnosis of laryngeal SCC. The data were correlated with age, pTNM, histological grade and recurrence. The tissues (tumor and adjacent tumor-free tissue) obtained during surgery were labeled with ³H-thymidine. A statistically significant difference was observed between tumoral tissue and adjacent tumor-free tissue (p < 0.05), but we could not confirm any statistically significant correlation between TLI and age, pTNM, histological grade and recurrence. In conclusion, TLI in laryngeal SCC had no significant relation with the clinicopathological features, probably due to the variation in tissue sampling and for tumor-dependent reasons. TLI may assist in differentiating malignant from benign lesions.

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DNA and its synthesis in individual tumor cells from human upper respiratory tract squamous cell carcinomas

Cited by 29 publications

References 18 publications

DNA content, malignancy grading and prognosis in T1 and T2 oral cavity carcinomas

DNA content, malignancy grading and prognosis in T1 and T2 oral cavity carcinomas

Flow Cytometry and Squamous Cell Carcinoma of the Maxillary Sinus: A Possible Prognostic Indicator for Multimodality Intervention

Thymidine Labeling Index in Laryngeal Squamous Cell Carcinoma in Correlation with pTNM, Age, Histological Grade and Recurrence

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