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Abstract: DNA adsorption on solid or liquid surfaces is a topic of broad fundamental and applied interest. Here, we study by X-ray reflectivity the adsorption of monodisperse double-stranded DNA molecules on a positively charged surface, obtained through chemical grafting of a homogeneous organic monomolecular layer of N-(2-aminoethyl) dodecanamide on an oxide-free monocrystalline Si(111) wafer. The adsorbed dsDNA is found to embed into the soft monolayer, which is deformed in the process. The surface coverage is very h… Show more

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“…The distribution of GelGreen labeled ssDNA would not be enriched in the benzyl benzonate droplet phase, similar to the case of fluorescein sodium as shown in Figure S3, but the natural characteristics of DNA would tend make it adsorbed at the interface [47][48][49] . The ssDNA might be concentrated further at the interface of the droplet to further enhance the fluorescence via increasing the amount of fluorophores close to the outer shell of the droplet, as shown in Figure 5a.…”
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“…The distribution of GelGreen labeled ssDNA would not be enriched in the benzyl benzonate droplet phase, similar to the case of fluorescein sodium as shown in Figure S3, but the natural characteristics of DNA would tend make it adsorbed at the interface [47][48][49] . The ssDNA might be concentrated further at the interface of the droplet to further enhance the fluorescence via increasing the amount of fluorophores close to the outer shell of the droplet, as shown in Figure 5a.…”
mentioning
“…The distribution of GelGreen labeled ssDNA would not be enriched in the benzyl benzonate droplet phase, similar to the case of fluorescein sodium as shown in Figure S3, but the natural characteristics of DNA would tend make it adsorbed at the interface. The ssDNA might be concentrated further at the interface of the droplet to further enhance the fluorescence via increasing the amount of fluorophores close to the outer shell of the droplet, as shown in Figure a. The DEF technique presents an opportunity to sensitively detect biomacromolecules, on the basis of their tendency to gather at the interface and thus further enhance the fluorescence of the droplet microresonator.…”
mentioning
“…At the beginning of the adsorption process in a monolayer of SH-DNA probes, the surface coverage is low, and the molecules of DNA reach the surface, filling the spaces randomly. As the surface coverage approaches saturation, neighbouring molecules will be led to more parallel orientations by the electrostatic repulsion of the already adsorbed molecules, finally obtaining tidy domains with a characteristic size given by the length of the rod-like dsDNA molecules, in a nematic order [32].…”
Section: E-field Attraction: Ssdna Inmobilization and Hybridizationmentioning
“…On the one hand, the attractive interactions lead to adsorption on a surface. The adsorption studied is done by x-ray reflectivity [8]. In some recent work [9], a theoretical analysis of such scenarios is based on the self-consistent field theory approach.…”
Section: Introductionmentioning