DNA adducts induced by <i>in vitro</i> activation of extracts of diesel and biodiesel exhaust particles

Ross, Jeffrey S.; Nelson, Garret B.; Mutlu, Esra; Warren, Sarah H.; Gilmour, M. Ian; DeMarini, David M.

doi:10.3109/08958378.2015.1068892

Cited by 17 publications

(5 citation statements)

References 25 publications

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“…Similar results were observed for organic extracts of diesel exhaust particles (DEP) in V79 and CHO cells, although the MN frequency was higher in CHO cells . The results obtained from ovary CHO‐K1 cells showed that the aromatic and slightly polar fraction of diesel exhaust PM extracts induced chromosomal damage and DNA breakage in a non‐cytotoxic dose . Another study investigated the synergistic effects of the extracts of DEP and ultraviolet A radiation (UVA) in human–hamster hybrid (A L ) cells.…”

Section: Discussionmentioning

confidence: 99%

“…Combustion of biodiesel usually results in lower emissions of carbon monoxide, hydrocarbons, PM and PAHs, but emissions of nitrogen oxides and aldehydes tended to be higher when compared with emissions from conventional fuels . Many authors strived to compare genotoxicity of conventional diesel and biodiesel fuels using diverse end‐points in various experimental systems including cellular models , mice , Salmonella Typhimurium or acellular calf thymus DNA system .…”

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confidence: 99%

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DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells

Cervena

Rossnerova

Sikorova

et al. 2017

Basic Clin Pharmacol Toxicol

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Internal combustion engine emissions belong among the major anthropogenic sources of air pollution in urban areas. According to the International Agency for Research on Cancer, there is sufficient evidence of the carcinogenicity of diesel exhaust in human beings. Although alternative fuels, mainly biodiesel, have recently become popular, little is still known about the genotoxicity of emissions from these fuels. We analysed DNA damage expressed as the frequency of micronuclei (MN) in human bronchial epithelial cells (BEAS-2B), induced by extractable organic matter (EOM; tested concentrations: 1, 10 and 25 μg/ml) obtained from particle emissions from various blends of biodiesel with diesel fuels (including neat diesel fuel (B0), a blend of 70% B0 and 30% biodiesel (B30) and neat biodiesel (B100)). We also tested the effect of selected diesel exhaust organic/genotoxic components [benzo[a]pyrene (B[a]P) concentrations: 25, 100 and 200 μM; 1-nitropyrene (1-NP) concentrations: 1, 5 and 10 μM; 3-nitrobenzanthrone (3-NBA) concentrations: 1, 5 and 50 μM]. The cells were treated with the compounds for 28 and 48 hr. Our results showed that most of the tested compounds (except for the 25 μM B[a]P, 28-hr treatment) significantly increased MN frequency. The genotoxicity of EOMs from the engine emissions of diesel and biodiesel engines was comparable. Both nitro-PAH compounds demonstrated higher genotoxic potential in comparison with B[a]P. Considering our results and due to increasing popularity of alternative fuels, it is prudent that the potential genotoxic effects of various fuels are investigated across engine technologies and operating conditions in a relevant model system.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells

Cervena

Rossnerova

Sikorova

et al. 2017

Basic Clin Pharmacol Toxicol

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“…RME particulates were less genotoxic than fossil diesel in 18 out of 32 comparisons with a pooled average ratio of 0.64. Comparisons for SME and HVO are inconsistent showing higher and lower potency depending on the blend level, however, only one article was suitable for SME (Ross et al 2015) therefore there is scope for further work with this biodiesel.…”

Section: Overview Of Genotoxic and Mutagenic Biomarkersmentioning

confidence: 99%

Re-assessing the toxicity of particles from biodiesel combustion: A quantitative analysis of in vitro studies

Southern

Hellier

Talibi

et al. 2021

Atmospheric Environment

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“…Air pollutants can act as endocrine disruptors through activation of the AhR or via ERs/ARs The main pollutants released by diesel fuels include ozone (O 3 ), NO 2 , and other nitrogen derivatives, sulfur derivatives, some traces of heavy metals (Pb and As), polycyclic aromatic hydrocarbons (PAH; benzene, benzopyrene) and particulate matter (PM). Biodiesel fuel can release nitro‐PAH and oxidized lipids of known toxicity by generation of DNA adducts (Ross et al, ). Occupational exposure to vinyl chloride also induces adduct formation in sperm DNA (Badouard et al, ).…”

Section: Air Pollution Hazards and Fertilitymentioning

confidence: 99%

The negative impact of the environment on methylation/epigenetic marking in gametes and embryos: A plea for action to protect the fertility of future generations

Menezo¹,

Dale²,

Elder

2019

Mol Reprod Dev

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Life expectancy has increased since World War II, and this may be attributed to several aspects of modern lifestyles. However, now we are faced with a downturn, which seems to be the result of environmental issues. This paradigm is paralleled with reduced human fertility, decreased sperm quality, increased premature ovarian failure, and diminished ovarian reserve syndromes. Endocrine disruptor chemicals and other toxic chemicals, herbicides, pesticides, plasticizers, to mention a few, are a rising concern in today's environment. Some of these are commonly used in the domestic setting: cleaning material and cosmetics and they have a known impact on epigenesis and imprinting via perturbation of methylation processes. Pollution from polyaromatic hydrocarbons, particulate matter <10 and <2.5 μm, and ozone released into the air, all affect fertility. Poor food processing management is a source of DNA adduct formation, which impairs the quality of gametes. An important concern is the nanoparticles that are present in food and are thought to induce oxidative stress. Now is the time to take a step backward. Global management of the environment and food production is required urgently to protect the fertility of future generations.

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DNA adducts induced by in vitro activation of extracts of diesel and biodiesel exhaust particles

Cited by 17 publications

References 25 publications

DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells

DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells

Re-assessing the toxicity of particles from biodiesel combustion: A quantitative analysis of in vitro studies

The negative impact of the environment on methylation/epigenetic marking in gametes and embryos: A plea for action to protect the fertility of future generations

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