Diversity of Sarcocystis spp shed by opossums in Brazil inferred with phylogenetic analysis of DNA coding ITS1, cytochrome B, and surface antigens

Valadas, Samantha Yuri Oshiro Branco; Silva, Juliana I.G. da; Lopes, Estela Gallucci; Keid, Lara Borges; Zwarg, Ticiana; Oliveira, Alice S.; Sanches, Tatiana; Joppert, Adriana Marques; Pena, Hilda Fátima Jesus; Oliveira, Tricia Maria Ferreira Souza; Ferreira, Helena Lage; Soares, Rodrigo Martins

doi:10.1016/j.exppara.2016.02.008

Cited by 23 publications

(36 citation statements)

References 34 publications

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“…Despite morphological and molecular similarities, S. neurona infects mammals predominantly, while S. falcatula exclusively infects birds. Two evolutionarily distant strains have been well recognized among isolates of S. falcatula in the Americas (Valadas et al 2016, Gondim et al 2017, Cesar et al 2018, and to our knowledge, a similar situation has not yet been detected in S. neurona, which appeared to be formed by only one genetic lineage. However, from the results presented here, it is probable that this diversity also occurs within S. neurona.…”

Section: Discussionmentioning

confidence: 73%

“…Complete internal transcribed spacer 1 was nested PCR amplified using the primer set described by Soares et al (2011). Finally, genetic sequences of SAG2, SAG3, and SAG4 were nested PCR amplified using the primers as designed in Monteiro et al (2013), Valadas et al (2016), and Gondim et al (2019). Primers used to amplify genetic sequences of Sarcocystis spp.…”

Section: Dna Extraction Pcr Amplification and Sequencingmentioning

confidence: 99%

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First molecular characterization of Sarcocystis neurona causing meningoencephalitis in a domestic cat in Brazil

et al. 2020

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Sarcocystis neurona is the main agent associated with equine protozoal myeloencephalitis (EPM). Apart from horses, S. neurona has been occasionally described causing neurologic disease in several other terrestrial animals as well as mortality in marine mammals. Herein, we describe the clinical, pathological, and molecular findings of a fatal case of S. neurona-associated meningoencephalitis in a domestic cat. The causing agent was analyzed by multilocus genotyping, confirming the presence of S. neurona DNA in the tissue samples of the affected animal. Significant molecular differences were found in relation to S. neurona isolates detected in other regions of the Americas. In addition, the parasite was identical to Sarcocystis sp. identified in opossum sporocysts in Brazil at molecular level, which suggests that transmission of. S. neurona in Brazil might involve variants of the parasite different from those found elsewhere in the Americas. Studies including more samples of S. neurona would be required to test this hypothesis, as well as to assess the impact of this diversity.

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Section: Discussionmentioning

confidence: 73%

Section: Dna Extraction Pcr Amplification and Sequencingmentioning

confidence: 99%

First molecular characterization of Sarcocystis neurona causing meningoencephalitis in a domestic cat in Brazil

et al. 2020

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“…Primers for cytochrome B coding sequences (cytB) targeting Sarcocystis spp. were designed from genomic sequences of S. neurona and S. falcatula (VALADAS et al, 2016). A CytB primer targeting members of the Toxoplasmatinae subfamily was designed from EST database sequences of N. caninum and RNAm sequences of T. gondii.…”

Section: Pcr and Primersmentioning

confidence: 99%

Molecular phylogeny of Toxoplasmatinae: comparison between inferences based on mitochondrial and apicoplast genetic sequences

Sercundes

Valadas

Keid

et al. 2016

Rev. Bras. Parasitol. Vet.

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Phylogenies within Toxoplasmatinae have been widely investigated with different molecular markers. Here, we studied molecular phylogenies of the Toxoplasmatinae subfamily based on apicoplast and mitochondrial genes. Partial sequences of apicoplast genes coding for caseinolytic protease (clpC) and beta subunit of RNA polymerase (rpoB), and mitochondrial gene coding for cytochrome B (cytB) were analyzed. Laboratory-adapted strains of the closely related parasites Sarcocystis falcatula and Sarcocystis neurona were investigated, along with Neospora caninum, Neospora hughesi, Toxoplasma gondii (strains RH, CTG and PTG), Besnoitia akodoni, Hammondia hammondi and two genetically divergent lineages of Hammondia heydorni. The molecular analysis based on organellar genes did not clearly differentiate between N. caninum and N. hughesi, but the two lineages of H. heydorni were confirmed. Slight differences between the strains of S. falcatula and S. neurona were encountered in all markers. In conclusion, congruent phylogenies were inferred from the three different genes and they might be used for screening undescribed sarcocystid parasites in order to ascertain their phylogenetic relationships with organisms of the family Sarcocystidae. The evolutionary studies based on organelar genes confirm that the genus Hammondia is paraphyletic. The primers used for amplification of clpC and rpoB were able to amplify genetic sequences of organisms of the genus Sarcocystis and organisms of the subfamily Toxoplasmatinae as well.Keywords: Sarcocystidae, molecular characterization, phylogeny, Toxoplasmatinae, apicoplast gene, mitochondrial gene. ResumoA filogenia da subfamília Toxoplasmatinae tem sido amplamente investigada com diversos marcadores moleculares. Neste estudo, a filogenia molecular da subfamília Toxoplasmatinae foi analisada através de genes de apicoplasto e mitocondriais. Foram analisadas sequências parciais de genes de apicoplasto codificadores da protease caseinolítica (clpC), e da subunidade beta da RNA polimerase (rpoB) e de gene mitocondrial codificador de citocromo B (cytB). Foram investigadas cepas adaptadas em laboratório de Sarcocystis neurona e Sarcocystis falcatula, parasitos estreitamente relacionados, além de Neospora caninum, Neospora hughesi, Toxoplasma gondii (cepas RH, CTG e PTG), Besnoitia akodoni, Hammondia hammondi e duas linhagens geneticamente divergentes de Hammondia heydorni. A análise molecular, baseada em genes de organelas, não diferenciou claramente N. caninum de N. hughesi, porém foi possível confirmar as duas linhagens de H. heydorni. Foram encontradas pequenas diferenças entre as cepas adaptadas em laboratório de

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“…A estreita relação entre S. neuroma com S. falcatula tem sido demonstrada por análise de sequência do gene do RNA ribossomal 18S (DAME et al, 1995;FENGER et al, 1995). Contudo, apesar das semelhanças, podem ser diferenciados biologicamente, S. falcatula não infectantando camundongos mas sim aves e S. neurona não infectando aves e sim camundongos OLIAS et al, 2014;VALADAS et al, 2016).…”

Section: Família Sarcocystidaeunclassified

“…As amostras cujas sequências ITS1 foram identificadas como S. falcatula-like foram submetidas à amplificação e sequenciamento de PCR com primers direcionados a SAG2, SAG3 e SAG4 (SAG -surface antigen gene) para diferenciação multilocus entre organismos S. falcatula-like VALADAS et al, 2016). LINDSAY et al, 2003;FAYER et al, 2004;DUBEY, 2009).…”

Section: Identificação Molecularunclassified

<b>Pesquisa de coccídios da família Sarcocystidae em pinguins-de-magalhães (<i>Spheniscus magellanicus</i>) encalhados na costa brasileira</b>

Acosta¹

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Jamais considere seus estudos como uma obrigação, mas como uma oportunidade invejável para aprender a conhecer a influência libertadora da beleza do reino do espírito, para seu próprio prazer pessoal e para proveito da comunidade à qual seu futuro trabalho pertencer" (Albert Einstein) RESUMO Acosta, I.C.L. Pesquisa de coccídios da família Sarcocystidae em pinguins-demagalhães (Spheniscus magellanicus) encalhados na costa brasileira.

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Diversity of Sarcocystis spp shed by opossums in Brazil inferred with phylogenetic analysis of DNA coding ITS1, cytochrome B, and surface antigens

Cited by 23 publications

References 34 publications

First molecular characterization of Sarcocystis neurona causing meningoencephalitis in a domestic cat in Brazil

First molecular characterization of Sarcocystis neurona causing meningoencephalitis in a domestic cat in Brazil

Molecular phylogeny of Toxoplasmatinae: comparison between inferences based on mitochondrial and apicoplast genetic sequences

<b>Pesquisa de coccídios da família Sarcocystidae em pinguins-de-magalhães (<i>Spheniscus magellanicus</i>) encalhados na costa brasileira</b>

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