Diversity ofVibrio parahaemolyticusin marine fishes of Bangladesh

Abstract

Aims: To determine the occurrence, diversity, antibiotic resistance and biofilm formation of Vibrio parahaemolyticus isolated from marine fishes in Bangladesh. Methods and Results: A total of 80 marine fishes were obtained from the local markets and examined for the presence of V. parahaemolyticus. All the isolated V. parahaemolyticus were characterized for the presence of virulence markers, thermostable direct hemolysin (TDH) or thermostable direct hemolysin related hemolysin (TRH). Isolates were serotyped and further characterized by enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) typing to analyse the genetic diversity. Moreover, biofilm formation and antibiotic resistance patterns were also determined. About 63Á75% (51/80) of the tested marine fishes were contaminated with V. parahaemolyticus. From the contaminated fishes, 71 representatives V. parahaemolyticus were isolated and none of them harboured tdh and trh virulence genes. Nine different O-groups and seven different K-types were found by serological analysis and the dominant serotype was O5:KUT. In ERIC-PCR analysis, eight clusters (A-H) were found and the most common pattern was A (46Á5%). All of the isolates were resistant to ampicillin and 78Á9% of isolates were resistant to streptomycin. The highest biofilm formation was found at 37°C compared to 25°C and 4°C. Conclusion: Diverse V. parahaemolyticus are present in marine fishes in the local market of Bangladesh with antibiotic-resistant properties and biofilm formation capacity. Significance and Impact of the Study: The widespread prevalence of diverse V. parahaemolyticus in marine fishes is an issue of serious concern, and it entails careful monitoring to ascertain the safety of seafood consumers.

Prophage-encoded gene VpaChn25_0734 amplifies ecological persistence of Vibrio parahaemolyticus CHN25

“…To investigate the influence of VpaChn25_0734 (543-bp) gene deletion on V. parahaemolyticus CHN25 survival, we determined growth curves of the WT, ΔVpaChn25_0734 (543-bp), and ΔVpaChn25_0734- com (543-bp) strains at 37 °C, 25 °C, and 15 °C, which V. parahaemolyticus experiences during life cycle (Maje et al 2020 ; Yang et al 2020 ; Ali et al 2021 ). At the optional growth temperature of 37 °C, the ΔVpaChn25_0734 (543-bp) mutant grew in TSB medium (pH 8.5, 3% NaCl) with a retardation phase (RP) of 2 h, when compared with the WT strain (Fig.…”

Vibrio parahaemolyticus from Migratory Birds in China Carries an Extra Copy of tRNA-Gly and Plasmid-Mediated Quinolone Resistance Gene qnrD

“…A possible reason for acquiring such an antibiotic resistance gene might be antibiotic overuse. Though a causal relationship between the use of conventional antibiotics and antibiotic resistance in V. parahaemolyticus has not been demonstrated and intrinsic resistance to some antibiotics, such as ampicillin, is possible ( 26 , 27 ), antibiotic resistance could be an indication of environmental enrichment through antibiotic contamination in wastewater and runoff or use in aquaculture ( 22 , 26 , 28 – 31 ). Also, even though the five PMQR gene-carried strains have not shown resistance to any antibiotics such as quinolone, a potential risk still exists; there could be circumstances during an infection by these strains where the PMQR gene qnrD confers antibiotic resistance, or this plasmid is transferred to other bacteria where it is clinically relevant.…”

Phenotypic characteristics and immune response of Procypris merus following challenge with aquatic isolate of Klebsiella pneumoniae

“…Diseases caused by bacterial infections are the most common, often resulting in the death of large numbers of farmed and wild aquatic animals, a major obstacle to the development of aquaculture (Sony et al., 2021). There is a wide variety of pathogens that cause bacterial diseases in aquatic animals, including Vibrio harveyi (Guzman et al., 2022), V. parahaemolyticus (Ali et al., 2021), Aeromonas hydrophila (Akmal et al., 2020), A. veronii (Chen et al., 2019), Edwardsiella tarda (Shao et al., 2016), Klebsiella pneumoniae (Sivaraman et al., 2020), Morganella morganii (Wei, Xiao et al., 2023), Photobacterium damselae (Terceti et al., 2016), etc. With the development of the breeding business, the expansion of the breeding scale, as well as the promotion of dense breeding, precision breeding technology, bacterial diseases are also constantly outbreaks of epidemic (Wei et al., 2023).…”