Diversity of bacterial endophytes in roots of Mexican husk tomato plants (Physalis ixocarpa) and their detection in the rhizosphere

Marquez-Santacruz, H. A.; Hernández-León, Rocío; Orozco-Mosqueda, MC; Velázquez-Sepúlveda, I.; Santoyo, Gustavo

doi:10.4238/vol9-4gmr921

Cited by 108 publications

(52 citation statements)

References 32 publications

(38 reference statements)

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“…The roots were washed with sterile distilled water and strongly adhered soil particles were carefully removed manually. Root tissue (1 g) specimens were obtained from each plant and surface sterilized using a method described by Márquez-Santacruz et al (2010). Briefly, the roots were immersed in 70% ethanol for 30 s, washed with fresh sodium hypochlorite solution (2.5% available Cl -) for 5 min, rinsed with 70% ethanol for 30 s, and finally washed five times with sterile distilled water.…”

Section: Plant Sampling and Bacterial Isolationmentioning

confidence: 99%

“…Previously reported PCR conditions were applied (Márquez-Santacruz et al, 2010). All PCR products were purified and directly sequenced at LANGEBIO (Irapuato, Mexico).…”

Section: Molecular Characterization Of Isolatesmentioning

confidence: 99%

“…Primary and lateral root cracks, as well as diverse tissue wounds resulting from plant growth, allow rhizospheric bacteria to penetrate and colonize the internal tissues of plants (Ali et al, 2014;Santoyo et al, 2016). Therefore, the rhizosphere is recognized as a reservoir of potential bacterial endophytes (Márquez-Santacruz et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Bacterial species belonging to other classes, such as Acidobacteria, Bacteroidetes, Planctomycetes, and Verrucomicrobia are less commonly found in plant tissues. Bacillus, Pseudomonas, Burkholderia, Stenotrophomonas, Micrococcus, Pantoea, and Microbacterium are among the most abundant and commonly reported genera of plant endophytes (Hallman et al, 1997;Márquez-Santacruz et al, 2010;Friesen et al, 2011;Xiong et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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A glimpse of the endophytic bacterial diversity in roots of blackberry plants (Rubus fruticosus)

Contreras-Pérez¹,

Loeza²,

Villegas³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to explore the diversity of culturable bacterial communities residing in blackberry plants (Rubus fruticosus). Bacterial endophytes were isolated from plant roots, and their 16S rDNA sequences were amplified and sequenced. Our results show that the roots of R. fruticosus exhibit low colony forming units of bacterial endophytes per gram of fresh tissue (6 x 10 2 ± 0.5 x 10 2 ). We identified 41 endophytic bacterial species in R. fruticosus by BLAST homology search and a subsequent phylogenetic analysis, belonging to the classes Actinobacteria, Bacilli, Alfaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. Predominantly, genera belonging the Proteobacteria (Burkholderia, 29.4%; Herbaspirillum, 10.7%; Pseudomonas, 4.9%; and Dyella, 3.9%), Firmicutes (Bacillus, 42.1%), and Actinobacteria (two isolates showing high identity with the Streptomyces genus, 1.9%) divisions were identified. Fifty percent of the bacterial endophytes produced the phytohormone indole-acetic acid (IAA), eleven of which exhibited higher IAA production (>5.8 mg/mL) compared to the plant growth-promoting strain, Pseudomonas fluorescens UM270. Additionally, the endophytic isolates exhibited protease activity (22%), produced siderophores (26.4%), and demonstrated antagonistic action (>50% inhibition of mycelial growth) against the grey mold phytopathogen Botrytis cinerea (3.9%). These results suggested that field-grown R. fruticosus plants contain bacterial endophytes within their tissues with the potential to promote plant growth and display antagonism towards plant pathogens.

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Section: Plant Sampling and Bacterial Isolationmentioning

confidence: 99%

“…Previously reported PCR conditions were applied (Márquez-Santacruz et al, 2010). All PCR products were purified and directly sequenced at LANGEBIO (Irapuato, Mexico).…”

Section: Molecular Characterization Of Isolatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A glimpse of the endophytic bacterial diversity in roots of blackberry plants (Rubus fruticosus)

Contreras-Pérez¹,

Loeza²,

Villegas³

et al. 2016

Genet. Mol. Res.

Self Cite

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show abstract

“…To confirm that the sterilization process was successful, water from the final rinse was tested for bacterial growth on LB medium plates. Uncontaminated tissues were then used for DNA extraction by the CTAB procedure, as described previously (Marquez-Santacruz et al, 2010).…”

Section: Sampling Of Plants and Dna Extractionmentioning

confidence: 99%

Diversity of endophytic bacteria in Caragana microphylla grown in the desert grassland of the Ningxia Hui Autonomous Region of China

2014

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ABSTRACT. The diversity of endophytic bacteria in the sand-fixation plant Caragana microphylla was investigated by amplified rDNA restriction analysis and by sequence and phylogenetic comparisons of the 16S rRNA genes. A total of 24, 19, and 17 operational taxonomic units were identified from 16S rDNA libraries of the plant roots, stems and leaves, respectively. Homology analysis revealed a 92-100% identity of bacterial 16S rDNA sequences compared with those in the GenBank database. The bacteria identified by sequence homology fell into the following groups: α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, bacilli, and uncultured bacterium. Sequence analysis demonstrated that the roots were colonized predominantly by Bradyrhizobiaceae, while bacteria from Burkholderiaceae and Sphingomonadaceae were predominant in the stems and leaves, respectively. Additionally, the endophytic bacterial community in leaves was more diverse than those in the roots and stems. Overall, the most abundant bacteria in all three tissues analyzed were from the Sphingomonadaceae and Burkholderiaceae families, although many bacterial populations were found in only a single tissue. These results suggest that the bacterial population of C. microphylla is diverse.

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Engineering and Management of Agricultural Microbiomes for Improving Crop Health

2023

Microbiomics and Sustainable Crop Production

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Diversity of bacterial endophytes in roots of Mexican husk tomato plants (Physalis ixocarpa) and their detection in the rhizosphere

Cited by 108 publications

References 32 publications

A glimpse of the endophytic bacterial diversity in roots of blackberry plants (Rubus fruticosus)

A glimpse of the endophytic bacterial diversity in roots of blackberry plants (Rubus fruticosus)

Diversity of endophytic bacteria in Caragana microphylla grown in the desert grassland of the Ningxia Hui Autonomous Region of China

Engineering and Management of Agricultural Microbiomes for Improving Crop Health

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