Diversity and structure of hyphomicrobium populations in a sewage treatment plant and its adjacent receiving lake

Abstract

Budding methylotrophic bacteria resembling Hyphomicrobium spp. were counted for 12 months in a German sewage treatment plant by most-probable-number (MPN) methods. Influent samples contained up to 2 ؋ 10 4 cells ml ؊1 , activated sludge consistently contained 1 ؋ 10 5 to 5 ؋ 10 5 cells ml ؊1 , and the effluent contained 1 ؋ 10 3 to 4 ؋ 10 3 cells ml ؊1 . The receiving lake had only 2 to 12 cells ml ؊1 . Six morphological groups with different growth requirements could be observed among 1,199 pure cultures that had been isolated from MPN dilutions. With dot blot DNA hybridizations, 671 isolates were assigned to 30 hybridization groups (HGs) and 84 could not be classified. Only HG 22 hybridized with a known species, Hyphomicrobium facilis IFAM B-522. Fourteen HGs (HGs 8 to 20 and HG 22) were specific for the lake; most others occurred only in the treatment plant. HGs 1, 3, and 26 were found in the activated sludge tank throughout the year, and HGs 27 and 28 were found for most of the year. In summary, it was demonstrated that bacteria with nearly identical and specific morphologies and nutritional types showed a high level of genetic diversity, although they were isolated under the same conditions and from the same treatment plant or its receiving lake. A directional exchange of these genetically different populations was possible but less significant, as was shown by the establishment of distinct populations in specific stations.

Quantification of Hyphomicrobium Populations in Activated Sludge from an Industrial Wastewater Treatment System as Determined by 16S rRNA Analysis

“…Hybridizations of samples taken from almost 1 year of operation indicated that the Hyphomicrobium cluster II organisms dominated over the Hyphomicrobium cluster I organisms by 3-to 12-fold. Holm et al (17) suggest that morphologically and nutritionally similar isolates of Hyphomicrobium may show a high level of genetic diversity and that Hyphomicrobium populations may vary seasonally. In this wastewater treatment plant the Hyphomicrobium populations showed genetic diversity but did not vary seasonally.…”

“…Hyphomicrobium spp. have been reported in both sewage treatment plants and adjacent waters (15,17,20). Recently, the 16S ribosomal DNA (rDNA) sequences for seven species of the genus Hyphomicrobium were published (30).…”

Natural Attenuation of Mn(II) in Metal Refinery Wastewater: Microbial Community Structure Analysis and Isolation of a New Mn(II)-Oxidizing Bacterium Pseudomonas sp. SK3

“…The second dominant genus (22.1%) was aerobic, methylotrophic budding bacteria Hyphomicrobium ( Figure 2) (mostly H. zavarzinii and H. hollandicum; Table S2), characteristic in producing hyphal filaments during growth. Hyphomicrobium has been widely detected as a dominant member in Mn-deposits from worldwide locations, including freshwater pipelines [19] and sewage treatment plants [17]. Despite its abundance in Mn-deposits, the difficulty in its isolation and steady maintenance makes it still unclear whether or not Hyphomicrobium is indeed directly responsible for Mn(II) oxidation [19,31].…”

“…Water 2019, 11, x FOR PEER REVIEW 7 of 15 member in Mn-deposits from worldwide locations, including freshwater pipelines [19] and sewage treatment plants [17]. Despite its abundance in Mn-deposits, the difficulty in its isolation and steady maintenance makes it still unclear whether or not Hyphomicrobium is indeed directly responsible for Mn(II) oxidation [19,31].…”

Development of a species-specific gene probe for Hyphomicrobium facilis with the inverse PCR

“…The genus Hyphomicrobium (18) with presently eight species (7,22) and additional 29 DNA-DNA hybridization groups (10,11) belongs to the alpha group of the Proteobacteria (21). Hyphomicrobium facilis, H. facilis subsp.…”